中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2012年
5期
481-484
,共4页
王皓%王俊杰%曲昂%刘敬佳%李金娜
王皓%王俊傑%麯昂%劉敬佳%李金娜
왕호%왕준걸%곡앙%류경가%리금나
西妥昔单抗%结直肠癌%CL187细胞%DNA损伤
西妥昔單抗%結直腸癌%CL187細胞%DNA損傷
서타석단항%결직장암%CL187세포%DNA손상
Cetuximab%Colorectal carcinoma%CL187 cell line%DNA damage
目的 研究西妥昔单抗(C225)联合外照射对结直肠癌CL187细胞生物学效应的影响,并探讨相关分子机制.方法 结直肠癌CL187细胞分单纯照射组和C225处理的联合照射组,受6MVX射线照射0、4和8 Gy后24和48 h,用四甲基偶氮唑盐(MTT)比色法测吸光度(A)值,比较两组细胞死亡率的差异.利用克隆形成实验比较两组细胞增殖能力的差异.采用流式细胞仪,检测细胞周期和细胞凋亡.蛋白免疫印迹法分析两组细胞DNA-PKcs、Ku70和Ku80蛋白表达量的变化.结果 联合照射组较单纯照射组死亡细胞比例增加(t=-6.14、-6.53,P＜0.05),细胞克隆形成能力下降.C225增强了射线对细胞的杀伤作用,放射增敏比SER为1.38.联合照射组G0/G1期细胞阻滞增加(t=-4.64,P＜0.05),细胞凋亡比例增加(t=-9.16,P＜0.05),DNA修复相关蛋白DNA-PKcs、Ku70和Ku80蛋白表达量减少.结论 西妥昔单抗增强照射对结直肠癌CL187细胞的杀伤作用,可能是通过影响细胞周期、细胞凋亡和DNA损伤修复基因实现的.
目的 研究西妥昔單抗(C225)聯閤外照射對結直腸癌CL187細胞生物學效應的影響,併探討相關分子機製.方法 結直腸癌CL187細胞分單純照射組和C225處理的聯閤照射組,受6MVX射線照射0、4和8 Gy後24和48 h,用四甲基偶氮唑鹽(MTT)比色法測吸光度(A)值,比較兩組細胞死亡率的差異.利用剋隆形成實驗比較兩組細胞增殖能力的差異.採用流式細胞儀,檢測細胞週期和細胞凋亡.蛋白免疫印跡法分析兩組細胞DNA-PKcs、Ku70和Ku80蛋白錶達量的變化.結果 聯閤照射組較單純照射組死亡細胞比例增加(t=-6.14、-6.53,P＜0.05),細胞剋隆形成能力下降.C225增彊瞭射線對細胞的殺傷作用,放射增敏比SER為1.38.聯閤照射組G0/G1期細胞阻滯增加(t=-4.64,P＜0.05),細胞凋亡比例增加(t=-9.16,P＜0.05),DNA脩複相關蛋白DNA-PKcs、Ku70和Ku80蛋白錶達量減少.結論 西妥昔單抗增彊照射對結直腸癌CL187細胞的殺傷作用,可能是通過影響細胞週期、細胞凋亡和DNA損傷脩複基因實現的.
목적 연구서타석단항(C225)연합외조사대결직장암CL187세포생물학효응적영향,병탐토상관분자궤제.방법 결직장암CL187세포분단순조사조화C225처리적연합조사조,수6MVX사선조사0、4화8 Gy후24화48 h,용사갑기우담서염(MTT)비색법측흡광도(A)치,비교량조세포사망솔적차이.이용극륭형성실험비교량조세포증식능력적차이.채용류식세포의,검측세포주기화세포조망.단백면역인적법분석량조세포DNA-PKcs、Ku70화Ku80단백표체량적변화.결과 연합조사조교단순조사조사망세포비례증가(t=-6.14、-6.53,P＜0.05),세포극륭형성능력하강.C225증강료사선대세포적살상작용,방사증민비SER위1.38.연합조사조G0/G1기세포조체증가(t=-4.64,P＜0.05),세포조망비례증가(t=-9.16,P＜0.05),DNA수복상관단백DNA-PKcs、Ku70화Ku80단백표체량감소.결론 서타석단항증강조사대결직장암CL187세포적살상작용,가능시통과영향세포주기、세포조망화DNA손상수복기인실현적.
Objective To investigate the combination effect of cetuximab and irradiation on colorectal carcinoma CL187 cell line and underlying molecular mechanism.Methods CL187 cells with or without cetuximab treatment were irradiated by 0,4 and 8 Gy X-rays,then cell death percentage was determined by MTT 24 and 48 h post-irradiation.Clone forming assay was used to evaluate the cell reproliferation ability.Cell cycle distribution,apoptosis,and necrosis were analyzed by flow cytometry.Western blot was used to detect the protein expressions of DNA-PKcs,Ku70 and Ku80.Results The cetuximab enhanced the percentage of radiation-induced cell death,while descreased the cloning formation capacity and increased radiosenvtivity (t =-6.14、-6.53,P ＜0.05).The SER of cetuximab on CL187 cell line approached to 1.38.In addition,cetuximab also increased radiation-induced G0/G1 phase arrest (t=-4.64,P＜0.05) and the percentage of apoptosis and necrosis (t=-9.16,P ＜0.05),but it descreased the expression levels of DNA-PKcs,Ku70 and Ku80 proteins.Conclusions The cetuximab treatment might enhance the inhibitory effect of irradiation on colorectal carcinoma CL187 cell line by influencing cell cycle distribution,cell apoptosis,and the expression of DNA repair proteins.
