CAJ | 학술논문

目的探讨饱和氢盐水对电离辐射诱发的脑组织损伤的保护作用.方法成年4月龄健康SD大鼠45只,采用随机数字表法分为健康对照组,盐水治疗组和氢水治疗组,再根据照射后时间分为1、3、7和14d4个时间组,每组5只.4 MeV电子线对大鼠进行单次垂直全脑照射,吸收剂量率为200 cGy/min,吸收剂量20 Gy.氢水治疗组大鼠于照射后即刻、照后连续3d给予饱和氢盐水腹腔注射,分别于照射后1、3、7和14 d处死大鼠,取脑组织制作匀浆,分光光度计检测超氧化物歧化酶(SOD)、丙二醛(MDA)、8-羟基脱氧鸟嘌呤(8-OHdG)含量.HE染色观察海马区脑组织病理变化.结果 7和14 d时氢水治疗组脑组织含水量明显小于盐水治疗组(t=3.78、3.18,P＜0.05),SOD水平1～7d时氢水治疗组明显高于盐水治疗组(t=2.41 ～2.92,P＜0.05),MDA水平明显低于盐水治疗组(t=4.01 ～6.03,P＜0.05),持续到14d,8-OHdG含量1～7d氢水治疗组明显低于盐水治疗组(t=2.33、2.71、2.33,P＜0.05).海马区神经细胞损伤明显轻于对照组.结论饱和氢盐水对大鼠急性放射性脑损伤早期有保护作用.
목적 탐토포화경염수대전리복사유발적뇌조직손상적보호작용.방법 성년4월령건강SD대서45지,채용수궤수자표법분위건강대조조,염수치료조화경수치료조,재근거조사후시간분위1、3、7화14d4개시간조,매조5지.4 MeV전자선대대서진행단차수직전뇌조사,흡수제량솔위200 cGy/min,흡수제량20 Gy.경수치료조대서우조사후즉각、조후련속3d급여포화경염수복강주사,분별우조사후1、3、7화14 d처사대서,취뇌조직제작균장,분광광도계검측초양화물기화매(SOD)、병이철(MDA)、8-간기탈양조표령(8-OHdG)함량.HE염색관찰해마구뇌조직병리변화.결과 7화14 d시경수치료조뇌조직함수량명현소우염수치료조(t=3.78、3.18,P＜0.05),SOD수평1～7d시경수치료조명현고우염수치료조(t=2.41 ～2.92,P＜0.05),MDA수평명현저우염수치료조(t=4.01 ～6.03,P＜0.05),지속도14d,8-OHdG함량1～7d경수치료조명현저우염수치료조(t=2.33、2.71、2.33,P＜0.05).해마구신경세포손상명현경우대조조.결론 포화경염수대대서급성방사성뇌손상조기유보호작용.
Objective To investigate the effect of hydrogen on radiation-induced acute injury in rat brain.Methods Forty-five mature Sprague-Dawley rats were randomly divided into three groups:saline therapy group,hydrogen therapy group and healthy control group.The whole brain of SD rat was irradiated with single dose of 20 Gy by 4 MeV electrons.Rats in therapy group were injected with hydrogen-rich saline after irradiation and were sacrificed at 1,3,7,14 d post-irradiation.The changes of malonaldehyde (MDA),superoxidase dismutase (SOD) and 8-hydroxydeoxygunosine (8-OHdG) in brain homogenate and the pathological changes in brain hippocampus were observed.Results The brain water content (t=3.78,3.18,P＜0.05) and the contents of 8-OHdG (t=2.33,2.71,2.33,P＜0.05) in the therapy group was lower than the control group at 7 d and 14 d post-irradiation.The contents of SOD were significantly higher(t =2.41-2.92,P ＜ 0.05) from 1 to 7 day,while the contents of MDA were significantly lower in therapy group than those in the control group from 1 to 14 day post-irradiation (t =4.01-6.20,P ＜ 0.05).Moreover,the damage degree in the nerve cells of hippocampus was less compared to the control group.Conclusions The hydrogen-rich saline could have protection role in irradiation-induced acute brain injury in rats.