中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2012年
6期
593-596
,共4页
孙威%倪新初%孙苏平%蔡雷铭%于静萍%王坚%聂斌%孙志强%倪昕晔%曹秀峰
孫威%倪新初%孫囌平%蔡雷銘%于靜萍%王堅%聶斌%孫誌彊%倪昕曄%曹秀峰
손위%예신초%손소평%채뢰명%우정평%왕견%섭빈%손지강%예흔엽%조수봉
新西兰兔%骨骼肌%放射损伤%电子线%超微结构
新西蘭兔%骨骼肌%放射損傷%電子線%超微結構
신서란토%골격기%방사손상%전자선%초미결구
Rabbits%Muscle skeletal%Radiation injury%Electron beam%Ultrastructure
目的 建立新西兰兔骨骼肌高剂量照射放射损伤模型,研究病理改变.方法 采用随机数字表法将28只新西兰兔分为照射组和对照组.照射组给予9 MeV电子线一次性照射单侧臀部80 Gy,分别于照射后1个月及6个月时取照射区骨骼肌组织,应用光镜和透射电镜观察其病理变化.结果 照射后1个月:光镜下肌细胞变性及坏死,肌间出血明显；电镜下肌细胞内见变性的肌原原纤维肿胀,明暗带结构不清,坏死区呈电子密度深浅不一的无定形区,线粒体肿胀、空泡变性.照射后6个月:光镜下肌细胞变性及坏死较1个月时明显,坏死区可见核碎裂,部分肌间血管管壁增厚、管腔闭塞,神经纤维退行性变,肌间纤维组织增生；电镜下变性区肌丝大量丢失,肌节萎缩,无定形坏死区范围增大,核内染色质减少、边集,胶原原纤维增生明显,线粒体异常增生.结论 9 MeV电子线一次性照射80 Gy照射兔骨骼肌后,骨骼肌细胞线粒体损伤、肌间血管及神经损伤可能是促进肌细胞及变性坏死的重要因素.
目的 建立新西蘭兔骨骼肌高劑量照射放射損傷模型,研究病理改變.方法 採用隨機數字錶法將28隻新西蘭兔分為照射組和對照組.照射組給予9 MeV電子線一次性照射單側臀部80 Gy,分彆于照射後1箇月及6箇月時取照射區骨骼肌組織,應用光鏡和透射電鏡觀察其病理變化.結果 照射後1箇月:光鏡下肌細胞變性及壞死,肌間齣血明顯；電鏡下肌細胞內見變性的肌原原纖維腫脹,明暗帶結構不清,壞死區呈電子密度深淺不一的無定形區,線粒體腫脹、空泡變性.照射後6箇月:光鏡下肌細胞變性及壞死較1箇月時明顯,壞死區可見覈碎裂,部分肌間血管管壁增厚、管腔閉塞,神經纖維退行性變,肌間纖維組織增生；電鏡下變性區肌絲大量丟失,肌節萎縮,無定形壞死區範圍增大,覈內染色質減少、邊集,膠原原纖維增生明顯,線粒體異常增生.結論 9 MeV電子線一次性照射80 Gy照射兔骨骼肌後,骨骼肌細胞線粒體損傷、肌間血管及神經損傷可能是促進肌細胞及變性壞死的重要因素.
목적 건립신서란토골격기고제량조사방사손상모형,연구병리개변.방법 채용수궤수자표법장28지신서란토분위조사조화대조조.조사조급여9 MeV전자선일차성조사단측둔부80 Gy,분별우조사후1개월급6개월시취조사구골격기조직,응용광경화투사전경관찰기병리변화.결과 조사후1개월:광경하기세포변성급배사,기간출혈명현；전경하기세포내견변성적기원원섬유종창,명암대결구불청,배사구정전자밀도심천불일적무정형구,선립체종창、공포변성.조사후6개월:광경하기세포변성급배사교1개월시명현,배사구가견핵쇄렬,부분기간혈관관벽증후、관강폐새,신경섬유퇴행성변,기간섬유조직증생；전경하변성구기사대량주실,기절위축,무정형배사구범위증대,핵내염색질감소、변집,효원원섬유증생명현,선립체이상증생.결론 9 MeV전자선일차성조사80 Gy조사토골격기후,골격기세포선립체손상、기간혈관급신경손상가능시촉진기세포급변성배사적중요인소.
Objective To establish a rabbit model of radiation-induced skeletal muscle injury in order to study the ultrastructural pathological changes and underlying mechanism.Methods 28 New Zealand rabbits were randomly divided into 2 groups with 16 rabbits in experimental group and 12 rabbits in control group.The experimental rabbits were irradiated on hip with a single dose of 80 Gy of 9 MeV electrons from a linear accelerator.1 month and 6 months after irradiation the pathological changes were respectively observed under light microscope and electron microscope.Results One month after irradiation,the morphologic changes including degeneration,necrosis of muscle cells,and hemorrhage between the muscle cells were observed under light microscope and the swelling of myofibrillae,blurring of light and shade band,vacuolar degeneration of mitochondria and amorphous areas of necrosis were observed under electron microscope.Six months after irradiation,the morphologic changes of nucleolus chips,fibrous connective tissue,thickening of vascular wall and vascular congestion between the muscle cells and the amorphous areas of necrosis in the experimental group were much more serious than those of 1 month after irradiation.In addition,the myofilaments were lost in degeneration areas and the sarcomere became shorten.Observation with electron microscope showed that the mitochondrial size and its morphological changes were varied and the amounts of collagen between myofibrillaes were increased 6 months after irradiation.Conclusions A rabbit model of high-dose irradiated skeleton muscle injury was successfully established with a single dose of 80 Gy of 9 MeV electrons from a linear accelerator.The degeneration and necrosis of muscle cells may be promoted by mitochondrial and vascular injury,degeneration of vessel and nerve fiber.
