中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2012年
6期
597-601
,共5页
金问森%肖林林%王娟%黄艳%徐师国%李俊
金問森%肖林林%王娟%黃豔%徐師國%李俊
금문삼%초림림%왕연%황염%서사국%리준
枇杷叶三萜酸%放射敏感性%肿瘤
枇杷葉三萜痠%放射敏感性%腫瘤
비파협삼첩산%방사민감성%종류
Triterpene acids of loquat leaf%Radiosensitivity%Tumor
目的 研究枇杷叶三萜酸对肿瘤细胞放射敏感性的影响及其作用机制.方法 采用枇杷叶三萜酸(TAL)预处理大鼠神经胶质瘤C6细胞和入骨肉瘤MG63细胞,经1、2、3、5和7 Gy的X射线照射后,通过细胞集落形成实验,计算TAL的放射增敏比(SER).采用细胞微核测定法,检测细胞微核率,观察DNA损伤情况.通过RT-PCR和Western blot观察细胞中Rad51和XRCC4的表达变化.结果 TAL作用后C6和MG63细胞的D0值分别降低至1.31和2.85 Gy,对C6和MG63细胞的SER值分别为1.73和2.04.与单纯照射组比较,当吸收剂量达到2 Gy以上时,TAL可导致两种肿瘤细胞的微核形成率增加(C6:t=-8.372--2.476,P<0.05;MG63:t=-4.03~-2.557,P<0.05).TAL并未显著抑制Rad51基因的表达水平,但可降低XRCC4在转录和翻译水平上的表达.结论 TAL可明显增加肿瘤细胞的放射敏感性,提高X射线对肿瘤细胞的杀伤效应,其作用机制可能与抑制照射后肿瘤细胞的非同源末端连接(NHEJ)修复有关.
目的 研究枇杷葉三萜痠對腫瘤細胞放射敏感性的影響及其作用機製.方法 採用枇杷葉三萜痠(TAL)預處理大鼠神經膠質瘤C6細胞和入骨肉瘤MG63細胞,經1、2、3、5和7 Gy的X射線照射後,通過細胞集落形成實驗,計算TAL的放射增敏比(SER).採用細胞微覈測定法,檢測細胞微覈率,觀察DNA損傷情況.通過RT-PCR和Western blot觀察細胞中Rad51和XRCC4的錶達變化.結果 TAL作用後C6和MG63細胞的D0值分彆降低至1.31和2.85 Gy,對C6和MG63細胞的SER值分彆為1.73和2.04.與單純照射組比較,噹吸收劑量達到2 Gy以上時,TAL可導緻兩種腫瘤細胞的微覈形成率增加(C6:t=-8.372--2.476,P<0.05;MG63:t=-4.03~-2.557,P<0.05).TAL併未顯著抑製Rad51基因的錶達水平,但可降低XRCC4在轉錄和翻譯水平上的錶達.結論 TAL可明顯增加腫瘤細胞的放射敏感性,提高X射線對腫瘤細胞的殺傷效應,其作用機製可能與抑製照射後腫瘤細胞的非同源末耑連接(NHEJ)脩複有關.
목적 연구비파협삼첩산대종류세포방사민감성적영향급기작용궤제.방법 채용비파협삼첩산(TAL)예처리대서신경효질류C6세포화입골육류MG63세포,경1、2、3、5화7 Gy적X사선조사후,통과세포집락형성실험,계산TAL적방사증민비(SER).채용세포미핵측정법,검측세포미핵솔,관찰DNA손상정황.통과RT-PCR화Western blot관찰세포중Rad51화XRCC4적표체변화.결과 TAL작용후C6화MG63세포적D0치분별강저지1.31화2.85 Gy,대C6화MG63세포적SER치분별위1.73화2.04.여단순조사조비교,당흡수제량체도2 Gy이상시,TAL가도치량충종류세포적미핵형성솔증가(C6:t=-8.372--2.476,P<0.05;MG63:t=-4.03~-2.557,P<0.05).TAL병미현저억제Rad51기인적표체수평,단가강저XRCC4재전록화번역수평상적표체.결론 TAL가명현증가종류세포적방사민감성,제고X사선대종류세포적살상효응,기작용궤제가능여억제조사후종류세포적비동원말단련접(NHEJ)수복유관.
Objective To study the increased radiosensitivity of tumor cells by triterpene acids of loquat leaf (TAL) and mechanism.Methods C6 and MG63 cells were pretreated by TAL,and then were exposed to X-rays at 1,2,3,5,7 Gy.Clonogenic assay was used to evaluate those tumor cells survival fraction (SF) to calculate the sensitization enhancement ratios (SER) of TAL.The cellular micronuclei ratios of tumor cells were analyzed by micronuclei assay.Additionally,the changes of Rad51 and XRCC4 levels were observed by RT-PCR and Western blot.Results D0 values were decreased to 1.31 and 2.85 Gy in C6 and MG63 cells by TAL pretreatment respectively.The SER value of the effect of TAL on C6 and MG63 cells was 1.73 and 2.04,respectively.There was a statistical difference in the cellular micronuclei ratios between tumor cells with TAL above 2 Gy and those without TAL (C6:t =-8.372--2.476,P <0.05 ; MG63:t =-4.03--2.557,P < 0.05).TAL attenuated the expression of XRCC4 at transcriptional and translational level,but not for Rad51,a key gene in homologous recombination repair (HRR).Conclusions TAL pretreatment could increase the lethal effect of X-rays on tumor cells in vitro.The mechanism might be involved in the inhibition of non-homologous end joining (NHEJ).