中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2013年
4期
373-375,387
,共4页
马辉%丁健华%张生彬%赵克
馬輝%丁健華%張生彬%趙剋
마휘%정건화%장생빈%조극
放射性肠炎%凉血愈肠汤%细胞核增殖抗原%Ki67%5-溴脱氧尿苷
放射性腸炎%涼血愈腸湯%細胞覈增殖抗原%Ki67%5-溴脫氧尿苷
방사성장염%량혈유장탕%세포핵증식항원%Ki67%5-추탈양뇨감
Radiation enteritis%Liang-Xue-Yu-Chang decoction%Proliferating cell nuclear antigen (PCNA)%Ki67%Bromodexyrldine (BrdU)
目的 探讨凉血愈肠汤灌胃治疗大鼠放射性肠炎的作用机制.方法 随机数字表法将60只雄性SD大鼠分为3组,正常对照组、蒸馏水组(10 Gy剂量照射后给予蒸馏水灌胃)和凉血愈肠汤组(10 Gy剂量照射后给予凉血愈肠汤灌胃),每组20只.连续7d分别给予照射后的大鼠蒸馏水和凉血愈肠汤灌胃,在实验结束前经腹腔注入5-溴脱氧尿苷(BrdU),采用SP方法及光镜观察肠腺上皮细胞增殖核抗原(PCNA)、Ki67和BrdU标记阳性细胞.结果 凉血愈肠汤组大鼠的肠上皮细胞PCNA、Ki67和BrdU标记阳性细胞数(22.0±2.7、71.2±5.7、26.2±5.9)显著多于蒸馏水组模型大鼠(11.2±1.9、61.6±5.5、11.3±2.2)(t=14.629、5.420、11.582,P＜0.05),少于正常对照组大鼠(30.4±5.7、86.6±5.1、32.3±3.2)(t=14.291、9.004、4.731,P＜0.05).结论 凉血愈肠汤可促进急性放射性肠炎大鼠肠上皮细胞的增殖.
目的 探討涼血愈腸湯灌胃治療大鼠放射性腸炎的作用機製.方法 隨機數字錶法將60隻雄性SD大鼠分為3組,正常對照組、蒸餾水組(10 Gy劑量照射後給予蒸餾水灌胃)和涼血愈腸湯組(10 Gy劑量照射後給予涼血愈腸湯灌胃),每組20隻.連續7d分彆給予照射後的大鼠蒸餾水和涼血愈腸湯灌胃,在實驗結束前經腹腔註入5-溴脫氧尿苷(BrdU),採用SP方法及光鏡觀察腸腺上皮細胞增殖覈抗原(PCNA)、Ki67和BrdU標記暘性細胞.結果 涼血愈腸湯組大鼠的腸上皮細胞PCNA、Ki67和BrdU標記暘性細胞數(22.0±2.7、71.2±5.7、26.2±5.9)顯著多于蒸餾水組模型大鼠(11.2±1.9、61.6±5.5、11.3±2.2)(t=14.629、5.420、11.582,P＜0.05),少于正常對照組大鼠(30.4±5.7、86.6±5.1、32.3±3.2)(t=14.291、9.004、4.731,P＜0.05).結論 涼血愈腸湯可促進急性放射性腸炎大鼠腸上皮細胞的增殖.
목적 탐토량혈유장탕관위치료대서방사성장염적작용궤제.방법 수궤수자표법장60지웅성SD대서분위3조,정상대조조、증류수조(10 Gy제량조사후급여증류수관위)화량혈유장탕조(10 Gy제량조사후급여량혈유장탕관위),매조20지.련속7d분별급여조사후적대서증류수화량혈유장탕관위,재실험결속전경복강주입5-추탈양뇨감(BrdU),채용SP방법급광경관찰장선상피세포증식핵항원(PCNA)、Ki67화BrdU표기양성세포.결과 량혈유장탕조대서적장상피세포PCNA、Ki67화BrdU표기양성세포수(22.0±2.7、71.2±5.7、26.2±5.9)현저다우증류수조모형대서(11.2±1.9、61.6±5.5、11.3±2.2)(t=14.629、5.420、11.582,P＜0.05),소우정상대조조대서(30.4±5.7、86.6±5.1、32.3±3.2)(t=14.291、9.004、4.731,P＜0.05).결론 량혈유장탕가촉진급성방사성장염대서장상피세포적증식.
Objective To study the effects of Liang-Xue-Yu-Chang decoction on intestinal epithelium of rats with radiation enteritis.Methods Sixty male SD rats were divided into three groups randomly:normal rats as control,abdominal radiation control group given distilled water after 10 Gy radiation,and abdominal radiation plus drug group given Liang-Xue-Yu-Chang decoction after 10 Gy radiation.Drugs or distilled water were chronically administered to animals for 7 days.After that,5-bromodexyridine (BrdU) was injected into abdominal cavity,SP immunohistochemistry was used to evaluate intestinal epithelium proliferating cell nuclear antigen (PCNA) and the positive cells labeled with Ki67 and BrdU.Results The number of intestinal epithelium cells with PCNA expression,positive Ki67 and BrdU were 22.0 + 2.7,71.2 + 5.7,and 26.2 + 5.9 respectively in the drug treatment group,significantly higher than those in the abdominal radiation control group (11.2 + 1.9,61.6 ± 5.5,and 11.3 ± 2.2) (t =14.629,5.420,11.582,P ＜ 0.05),but lower than those in the normal control group (30.4±5.7,86.6 ±5.1,and 32.3 ±3.2)(t =14.291,9.004,4.731,P＜0.05).Conclusion Liang-Xue-Yu-Chang decoction could improve the proliferation of intestinal epithelium in the rats with radiation enteritis.
