CAJ | 학술논문

目的了解肌肽对小鼠放射性肺损伤的防治作用.方法近交系C57/BL雌性小鼠108只,随机数字表法分为对照组、单纯照射组、照射+肌肽组(15 mg·kg-1·d-1)和单纯肌肽组(15mg· kg-1·d-1),对照组共18只,其他各组每组30只.采用10 MV X射线经前胸单次照射小鼠肺脏中平面13 Gy,全肺照射.两肌肽组灌胃给予15 mg·kg-1 ·d-1肌肽,照射前30 min给药1次,以后1次/d至实验结束,对照组给予相应同体积生理盐水.在照射后7、28和56 d处死后取部分肺组织HE染色,部分肺组织行超氧化物歧化酶(SOD)检测,同时ELISA法测定血清中TGF-β1、TNF-α.结果受照射组(单纯照射组、照射+肌肽组)在照射后56 d均表现出不同程度的放射性肺炎病理变化,照射+给药组较单纯照射组炎症反应明显较轻.各组间不同时段血清中TGF-β1水平差异有统计学意义(F=10.65、3.70、4.04,P＜0.05);各组间不同时段血清中TNF-α水平差异有统计学意义(F =39.55、53.38、4.01,P＜0.05);各组间不同时段肺组织中SOD水平差异有统计学意义(F=4.33、4.19、3.34,P＜0.05).照射+肌肽组较同期的单纯照射组,肺组织中SOD水平明显较高,血清中TGF-β1、TNF-α明显降低.结论肌肽通过保护SOD,降低TGF-β1和TNF-α水平起到预防并减轻小鼠放射性肺损伤的作用.
목적 료해기태대소서방사성폐손상적방치작용.방법 근교계C57/BL자성소서108지,수궤수자표법분위대조조、단순조사조、조사+기태조(15 mg·kg-1·d-1)화단순기태조(15mg· kg-1·d-1),대조조공18지,기타각조매조30지.채용10 MV X사선경전흉단차조사소서폐장중평면13 Gy,전폐조사.량기태조관위급여15 mg·kg-1 ·d-1기태,조사전30 min급약1차,이후1차/d지실험결속,대조조급여상응동체적생리염수.재조사후7、28화56 d처사후취부분폐조직HE염색,부분폐조직행초양화물기화매(SOD)검측,동시ELISA법측정혈청중TGF-β1、TNF-α.결과 수조사조(단순조사조、조사+기태조)재조사후56 d균표현출불동정도적방사성폐염병리변화,조사+급약조교단순조사조염증반응명현교경.각조간불동시단혈청중TGF-β1수평차이유통계학의의(F=10.65、3.70、4.04,P＜0.05);각조간불동시단혈청중TNF-α수평차이유통계학의의(F =39.55、53.38、4.01,P＜0.05);각조간불동시단폐조직중SOD수평차이유통계학의의(F=4.33、4.19、3.34,P＜0.05).조사+기태조교동기적단순조사조,폐조직중SOD수평명현교고,혈청중TGF-β1、TNF-α명현강저.결론 기태통과보호SOD,강저TGF-β1화TNF-α수평기도예방병감경소서방사성폐손상적작용.
Objective To investigate the protective effect of carnosine on radiation-induced lung injury in mice.Methods A total of 108 C57/BL female mice were randomly divided into 4 groups:control group without treatment,irradiation alone group,irradiation + carnosine group (15 mg·kg-1·d-1),and carnosine alone group (15 mg· kg-1· d-1).There were 18 mice in control group and 30 mice in every other group.Whole lung anterior chest was irradiated with a single dose of 13 Gy 10 MV X-rays.The mice were administered with carnosine (15 mg· kg-1· d-1) at 30 minutes before irradiation and then garaged once a day until the end of the experiment.The control group was given with saline.At 7,28,and 56 d after irradiation,6 mice of control group and 10 mice of each other group were killed.A portion of lung tissues were stained with HE and other part of lung tissues were used to detect the levels of SOD.Meanwhile,TGF-β1 and TNF-α in the serum were detected with ELISA.Results Different levels of inflammation factors were expressed in the lung tissues of irradiation group and irradiation + carnosine group at 56 d after irradiation,but the inflammation in the irradiation + carnosine group was significantly lighter than that in the irradiation group.Among (7,28 and 56 d) after radiation,TGF-β1,TNF-α,and SOD levels in different groups had significant differences.At the same time point after irradiation,the level of SOD in lung tissue of irradiation + carnosine group was significantly higher than that of irradiation group (F =4.33,4.19,3.34,P ＜0.05),but the levels of TGF-β1 and TNF-α in serum were reduced.Conclusions Carnosine can prevent and inhibit radiation-induced lung injury in mice by increasing SOD and reducing TGF-β1 and TNF-α.