中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2014年
1期
8-12
,共5页
刘静%唐益庭%周俊东%张舒羽%曹晗%吴锦昌%罗居东%陈光烈%曹建平
劉靜%唐益庭%週俊東%張舒羽%曹晗%吳錦昌%囉居東%陳光烈%曹建平
류정%당익정%주준동%장서우%조함%오금창%라거동%진광렬%조건평
蛋白酶体抑制剂%MG132%肺腺癌%细胞生长%转移%细胞周期阻滞
蛋白酶體抑製劑%MG132%肺腺癌%細胞生長%轉移%細胞週期阻滯
단백매체억제제%MG132%폐선암%세포생장%전이%세포주기조체
Proteasome inhibitor%MG132%Lung adenocarcinoma%Cell growth%Metastasis%Cell cycle arrest
目的 研究蛋白酶体抑制剂MG132联合X射线对人肺腺癌A549细胞生长、迁移、侵袭、细胞周期分布的影响及机制.方法 MTT法检测不同MG132浓度处理后不同时间肺腺癌A549细胞的增殖;克隆形成实验检测A549细胞的存活能力;划痕实验测定A549细胞的迁移能力;Transwell小室测定其侵袭能力;流式细胞术测定细胞周期分布的改变;Western blot法测定蛋白表达水平.结果 MG132明显抑制人肺腺癌A549细胞的生长,并呈现剂量-效应和时间-效应关系.MG132联合X射线对A549细胞克隆存活能力有显著抑制作用(F=554.78、954.64,P<0.01),且加MG132组克隆存活率均低于照射组(t=4.44、12.41、3.52、6.72,P<0.05).MG132在无毒性剂量下联合X射线可显著抑制A549细胞的迁移及侵袭能力(t=12.79,P<0.01),并明显增加G1期细胞阻滞(t=4.29,P<0.05);MG132联合X射线明显降低A549细胞中基质金属蛋白酶-2,-9和G1期相关蛋白Cyclin D1的表达水平,同时增加细胞中P53的表达.结论 MG132可以显著抑制人肺腺癌A549细胞的生长,且在无毒性剂量下联合X射线可以明显降低其转移和侵袭能力,显著增加肿瘤细胞的G1期阻滞.
目的 研究蛋白酶體抑製劑MG132聯閤X射線對人肺腺癌A549細胞生長、遷移、侵襲、細胞週期分佈的影響及機製.方法 MTT法檢測不同MG132濃度處理後不同時間肺腺癌A549細胞的增殖;剋隆形成實驗檢測A549細胞的存活能力;劃痕實驗測定A549細胞的遷移能力;Transwell小室測定其侵襲能力;流式細胞術測定細胞週期分佈的改變;Western blot法測定蛋白錶達水平.結果 MG132明顯抑製人肺腺癌A549細胞的生長,併呈現劑量-效應和時間-效應關繫.MG132聯閤X射線對A549細胞剋隆存活能力有顯著抑製作用(F=554.78、954.64,P<0.01),且加MG132組剋隆存活率均低于照射組(t=4.44、12.41、3.52、6.72,P<0.05).MG132在無毒性劑量下聯閤X射線可顯著抑製A549細胞的遷移及侵襲能力(t=12.79,P<0.01),併明顯增加G1期細胞阻滯(t=4.29,P<0.05);MG132聯閤X射線明顯降低A549細胞中基質金屬蛋白酶-2,-9和G1期相關蛋白Cyclin D1的錶達水平,同時增加細胞中P53的錶達.結論 MG132可以顯著抑製人肺腺癌A549細胞的生長,且在無毒性劑量下聯閤X射線可以明顯降低其轉移和侵襲能力,顯著增加腫瘤細胞的G1期阻滯.
목적 연구단백매체억제제MG132연합X사선대인폐선암A549세포생장、천이、침습、세포주기분포적영향급궤제.방법 MTT법검측불동MG132농도처리후불동시간폐선암A549세포적증식;극륭형성실험검측A549세포적존활능력;화흔실험측정A549세포적천이능력;Transwell소실측정기침습능력;류식세포술측정세포주기분포적개변;Western blot법측정단백표체수평.결과 MG132명현억제인폐선암A549세포적생장,병정현제량-효응화시간-효응관계.MG132연합X사선대A549세포극륭존활능력유현저억제작용(F=554.78、954.64,P<0.01),차가MG132조극륭존활솔균저우조사조(t=4.44、12.41、3.52、6.72,P<0.05).MG132재무독성제량하연합X사선가현저억제A549세포적천이급침습능력(t=12.79,P<0.01),병명현증가G1기세포조체(t=4.29,P<0.05);MG132연합X사선명현강저A549세포중기질금속단백매-2,-9화G1기상관단백Cyclin D1적표체수평,동시증가세포중P53적표체.결론 MG132가이현저억제인폐선암A549세포적생장,차재무독성제량하연합X사선가이명현강저기전이화침습능력,현저증가종류세포적G1기조체.
Objective To study the effects of proteasome inhibitor MG132 on the growth,metastasis,and cell cycle distribution of human lung adenocarcinoma cells A549 irradiated by X-rays.Methods After treatment of MG132 and irradiation,cell proliferation was detected by MTT assay.Survival was measured by clonogenic assay.Cell migration ability was detected by the Scratch migration assay.Cell invasion ability was detected by transwell migration assay.Cell cycle distribution were analyzed by flow cytometry assay.Protein expression was detected by Western blot assay.Results MG132 alone inhibited cell growth in a dose-and time-dependent manner.MG132 in combination with radiation significantly suppressed the growth,migration and invasion of A549 cells compared to the control (F =554.78,954.64,P < 0.01).MG132 enhanced radiation-induced G1-arrest (t =4.44,12.41,3.52,6.72,P <0.05).The G1 cell cycle distribution rate of MG132 plus RT group was increased to (71.05 ± 4.17)%.The expressions of MMP-2,MMP-9 and Cyclin D1 were significantly suppressed by MG132 in combination with radiation,while the expression of P53 was up-regulated.Conclusions MG132 inhibits cell growth,migration and invasion ability,and induces G1 cell cycle arrest of A549 cells treated with MG132 in combination with radiation,in which the down-regulation of MMPs and Cyclin D1 and upregulation of P53 may be involved.