中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2014年
1期
13-17
,共5页
郭艳婷%王彦%杜利清%徐畅%曹嘉%刘建香%陈凤华%付岳%刘强
郭豔婷%王彥%杜利清%徐暢%曹嘉%劉建香%陳鳳華%付嶽%劉彊
곽염정%왕언%두리청%서창%조가%류건향%진봉화%부악%류강
Tat-SmacN7%乳腺癌%放射增敏%细胞凋亡%凋亡抑制蛋白
Tat-SmacN7%乳腺癌%放射增敏%細胞凋亡%凋亡抑製蛋白
Tat-SmacN7%유선암%방사증민%세포조망%조망억제단백
Tat-SmacN7%Breast cancer%Radiosensitization%Cell apoptosis%Inhibitor of apoptosis proteins
目的 观察Tat-SmacN7对人乳腺癌SK-BR-3细胞放射敏感性的影响并探讨其机制.方法 取对数生长期乳腺癌SK-BR-3细胞,分为对照组、γ射线照射组、Tat-SmacN7组和Tat-SmacN7联合γ射线照射组(联合组).MTT法检测Tat-SmacN7对SK-BR-3细胞的毒性;克隆形成实验检测Tat-SmacN7的存活分数;单击多靶模型拟合细胞存活曲线并计算放射增敏比(SER);流式细胞术检测Tat-SmacN7联合γ射线照射对细胞凋亡的影响;Western blot法检测细胞凋亡抑制蛋白XIAP和cIAP-1蛋白表达水平.结果 Tat-SmacN7随浓度升高对SK-BR-3细胞的增殖抑制率增大,药物浓度与细胞增殖率呈正相关(r=0.924,P＜0.05),IC50为(62.62±1.19) μmol/L,IC20为(5.66±0.67) μmol/L;5 μmol/L Tat-SmacN7能增加SK-BR-3细胞的放射敏感性,联合组与照射组放射增敏比为1.48;6 Gyγ射线照射后48 h,联合组凋亡率显著高于照射组(t=7.01,P＜0.05);与对照组相比,Tat-SmacN7组XIAP表达水平降低,联合组XIAP和cIAP-1蛋白表达水平均降低.结论 Tat-SmacN7通过促进人乳腺癌细胞SK-BR-3细胞的凋亡,增加其辐射敏感性,这一特性与XIAP的表达有关.
目的 觀察Tat-SmacN7對人乳腺癌SK-BR-3細胞放射敏感性的影響併探討其機製.方法 取對數生長期乳腺癌SK-BR-3細胞,分為對照組、γ射線照射組、Tat-SmacN7組和Tat-SmacN7聯閤γ射線照射組(聯閤組).MTT法檢測Tat-SmacN7對SK-BR-3細胞的毒性;剋隆形成實驗檢測Tat-SmacN7的存活分數;單擊多靶模型擬閤細胞存活麯線併計算放射增敏比(SER);流式細胞術檢測Tat-SmacN7聯閤γ射線照射對細胞凋亡的影響;Western blot法檢測細胞凋亡抑製蛋白XIAP和cIAP-1蛋白錶達水平.結果 Tat-SmacN7隨濃度升高對SK-BR-3細胞的增殖抑製率增大,藥物濃度與細胞增殖率呈正相關(r=0.924,P＜0.05),IC50為(62.62±1.19) μmol/L,IC20為(5.66±0.67) μmol/L;5 μmol/L Tat-SmacN7能增加SK-BR-3細胞的放射敏感性,聯閤組與照射組放射增敏比為1.48;6 Gyγ射線照射後48 h,聯閤組凋亡率顯著高于照射組(t=7.01,P＜0.05);與對照組相比,Tat-SmacN7組XIAP錶達水平降低,聯閤組XIAP和cIAP-1蛋白錶達水平均降低.結論 Tat-SmacN7通過促進人乳腺癌細胞SK-BR-3細胞的凋亡,增加其輻射敏感性,這一特性與XIAP的錶達有關.
목적 관찰Tat-SmacN7대인유선암SK-BR-3세포방사민감성적영향병탐토기궤제.방법 취대수생장기유선암SK-BR-3세포,분위대조조、γ사선조사조、Tat-SmacN7조화Tat-SmacN7연합γ사선조사조(연합조).MTT법검측Tat-SmacN7대SK-BR-3세포적독성;극륭형성실험검측Tat-SmacN7적존활분수;단격다파모형의합세포존활곡선병계산방사증민비(SER);류식세포술검측Tat-SmacN7연합γ사선조사대세포조망적영향;Western blot법검측세포조망억제단백XIAP화cIAP-1단백표체수평.결과 Tat-SmacN7수농도승고대SK-BR-3세포적증식억제솔증대,약물농도여세포증식솔정정상관(r=0.924,P＜0.05),IC50위(62.62±1.19) μmol/L,IC20위(5.66±0.67) μmol/L;5 μmol/L Tat-SmacN7능증가SK-BR-3세포적방사민감성,연합조여조사조방사증민비위1.48;6 Gyγ사선조사후48 h,연합조조망솔현저고우조사조(t=7.01,P＜0.05);여대조조상비,Tat-SmacN7조XIAP표체수평강저,연합조XIAP화cIAP-1단백표체수평균강저.결론 Tat-SmacN7통과촉진인유선암세포SK-BR-3세포적조망,증가기복사민감성,저일특성여XIAP적표체유관.
Objective To investigate the radiosensitive effect of Tat-SmacN7 on human breast cancer cell line of SK-BR-3 and the underlying mechanism.Methods SK-BR-3 cells in the logarithmic phase were divided into 4 groups:control group,drug group,137Cs γ-ray radiation group and the case group (γ-ray radiation + Tat-SmacN7).MTT assay was performed to evaluate the cytotoxicity of Tat-SmacN7.Colony formation assay was used to measure cell survival fraction.A single-hit multi-target model was used to fit the survival curve and calculate the sensitive enhancement ratio (SER).Apoptosis rate was analyzed by using flow cytometry.The expressions of inhibitor of apoptosis proteins(IAP) including XIAP,cIAP-1 proteins were detected by Western blot method.Results Tat-SmacN7 inhibited cell growth in a dose-dependent manner(r =0.924,P ＜ 0.05) and its 50％ inhibition concentration (IC50) was (62.62 ± 1.19) μmol/L,and IC20 was (5.66 ± 0.67) μmol/L.In addition,5 μmol/L Tat-SmacN7 could enhance cell radiosensitivity with a SER of 1.48.After 6 Gy γ-ray radiation at 48 h,the incidence of apoptosis in the case group was higher than that in the irradiation alone group (t =7.01,P ＜ 0.05).Tat-SmacN7 only inhibited XIAP expression,whereas Tat-SmacN7 combined with radiation inhibited cIAP-1 as well.Conclusions Tat-SmacN7 has a radiosensitization effect on human breast cancer SK-BR-3 cell by promoting apoptosis,which might be related with XIAP expression.
