中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2014年
1期
18-21
,共4页
史涛%董娟聪%张婉泽%武宁%邵明龙%卢良杰%张聪%张海芹%单玉兴
史濤%董娟聰%張婉澤%武寧%邵明龍%盧良傑%張聰%張海芹%單玉興
사도%동연총%장완택%무저%소명룡%로량걸%장총%장해근%단옥흥
电离辐射%ICOS/ICOSL%NF-κB%IL-10%信号通路
電離輻射%ICOS/ICOSL%NF-κB%IL-10%信號通路
전리복사%ICOS/ICOSL%NF-κB%IL-10%신호통로
Ionizing irradiation%ICOS/ICOSL%NF-κB%IL-10%Signaling pathway
目的 观察不同剂量X射线照射后小鼠脾细胞可诱导共刺激分子及其配体(ICOS/ICOSL)与核因子NF-κB、细胞因子IL-10表达量的变化.方法 健康ICR小鼠按随机数字表法分为低剂量照射组(0.05、0.075和0.2 Gy)、高剂量照射组(1、2、4和6 Gy)和假照组.假照组于假照后16 h处死,高、低剂量照射组分别于照后0、4、8、16、24、48、72 h处死小鼠,分离小鼠脾脏制成单细胞悬液,提取脾组织总蛋白,采用流式细胞术检测ICOS/ICOSL,Western blot法检测NF-κB蛋白水平,采用ELISA法检测IL-10分泌量的变化.结果 在0.05、0.075 Gy低剂量照射后,ICOS/ICOSL双阳性细胞百分比显著低于假照组(t =4.743、4.120,P＜0.01);在4、6 Gy高剂量照射后,则上调其表达(t=-4.950、-7.310,P＜0.01).核因子NF-κB变化趋势与ICOS/ICOSL表达变化相似.IL-10在0.075、0.2 Gy低剂量照射后明显低于假照组(t=5.277、2.854,P＜0.05),但在6 Gy照射后明显高于假照组(t =7.196,P＜0.01).结论 低剂量电离辐射抑制ICOS/ICOSL、NF-κB和IL-10的表达,而高剂量辐射上调ICOS/ICOSL表达,并激活核因子NF-κB,进而导致IL-10分泌量升高.
目的 觀察不同劑量X射線照射後小鼠脾細胞可誘導共刺激分子及其配體(ICOS/ICOSL)與覈因子NF-κB、細胞因子IL-10錶達量的變化.方法 健康ICR小鼠按隨機數字錶法分為低劑量照射組(0.05、0.075和0.2 Gy)、高劑量照射組(1、2、4和6 Gy)和假照組.假照組于假照後16 h處死,高、低劑量照射組分彆于照後0、4、8、16、24、48、72 h處死小鼠,分離小鼠脾髒製成單細胞懸液,提取脾組織總蛋白,採用流式細胞術檢測ICOS/ICOSL,Western blot法檢測NF-κB蛋白水平,採用ELISA法檢測IL-10分泌量的變化.結果 在0.05、0.075 Gy低劑量照射後,ICOS/ICOSL雙暘性細胞百分比顯著低于假照組(t =4.743、4.120,P＜0.01);在4、6 Gy高劑量照射後,則上調其錶達(t=-4.950、-7.310,P＜0.01).覈因子NF-κB變化趨勢與ICOS/ICOSL錶達變化相似.IL-10在0.075、0.2 Gy低劑量照射後明顯低于假照組(t=5.277、2.854,P＜0.05),但在6 Gy照射後明顯高于假照組(t =7.196,P＜0.01).結論 低劑量電離輻射抑製ICOS/ICOSL、NF-κB和IL-10的錶達,而高劑量輻射上調ICOS/ICOSL錶達,併激活覈因子NF-κB,進而導緻IL-10分泌量升高.
목적 관찰불동제량X사선조사후소서비세포가유도공자격분자급기배체(ICOS/ICOSL)여핵인자NF-κB、세포인자IL-10표체량적변화.방법 건강ICR소서안수궤수자표법분위저제량조사조(0.05、0.075화0.2 Gy)、고제량조사조(1、2、4화6 Gy)화가조조.가조조우가조후16 h처사,고、저제량조사조분별우조후0、4、8、16、24、48、72 h처사소서,분리소서비장제성단세포현액,제취비조직총단백,채용류식세포술검측ICOS/ICOSL,Western blot법검측NF-κB단백수평,채용ELISA법검측IL-10분비량적변화.결과 재0.05、0.075 Gy저제량조사후,ICOS/ICOSL쌍양성세포백분비현저저우가조조(t =4.743、4.120,P＜0.01);재4、6 Gy고제량조사후,칙상조기표체(t=-4.950、-7.310,P＜0.01).핵인자NF-κB변화추세여ICOS/ICOSL표체변화상사.IL-10재0.075、0.2 Gy저제량조사후명현저우가조조(t=5.277、2.854,P＜0.05),단재6 Gy조사후명현고우가조조(t =7.196,P＜0.01).결론 저제량전리복사억제ICOS/ICOSL、NF-κB화IL-10적표체,이고제량복사상조ICOS/ICOSL표체,병격활핵인자NF-κB,진이도치IL-10분비량승고.
Objective To observe the changes of inducible co-stimulator and ligand ICOS/ ICOSL),nuclear factor NF-κB,and cytokine IL-10 in mouse spleen after different doses of ionizing radiation.Methods The healthy ICR mice were randomly divided into low dose irradiation groups with 0.05,0.075 and 0.2 Gy and high dose irradiation groups with 1,2,4 and 6 Gy,respectively.The control mice were killed 16 h after sham-irradiation and the irradiated mice were killed at 0,4,8,16,24,48,72 h after irradiation.Mice spleen was taken out to prepare for single cell suspension and to extract total protein.The expression of ICOS/ICOSL was detected using flow cytometry.The expressions of NF-κB and [L-10 were detected by Western blot and ELISA,respectively.Results After low doses of 0.05 and 0.075 Gy,the percentage of ICOS/ICOSL double positive cells was significantly lower than that of shamirradiation (t =4.743,4.120,P ＜ 0.01).The expression of ICOS/ICOSL was increased after high doses of 4 and 6 Gy irradiation (t =-4.950,-7.310,P ＜ 0.01).The NF-κB expression had a similar tendency with ICOS/ICOSL.But the secretion of IL-10 was decreased after low dose of 0.075 and 0.2 Gy irradiation (t =5.277,2.854,P ＜ 0.05),but increased after 6 Gy irradiation compared with shamirradiation group (t =7.196,P ＜ 0.01).Conclusions Low dose irradiation inhibits the expressions of ICOS/ICOSL,NF-κB and IL-10,while high dose irradiation inscreases the expression of ICOS/ICOSL and activates NF-κB then increases the secretion of IL-10.
