CAJ | 학술논문

目的探讨2,2-二甲基四氢噻唑盐酸盐(抗放利)对辐射诱导人外周血淋巴细胞遗传损伤的防护作用.方法采集健康人外周血,分为健康对照组、单纯照射组和抗放利防护组.照射剂量为2 Gy.抗放利防护组分0.2、0.5、1、2 mmol/L 4个浓度组,于照前30 min给药.通过染色体畸变分析、微核及单细胞凝胶电泳检测,观察抗放利对辐射致人外周血淋巴细胞遗传损伤的防护作用.结果各浓度抗放利防护组与单纯照射组比较,染色体畸变率(t=5.34 ～25.48,P＜0.05)、微核细胞率(t=5.18～29.44,P＜0.05)与微核率(=4.67～12.04,P＜0.05)、彗星细胞尾长(t=16.18～ 19.64,P＜0.05)、尾矩(t=11.79 ～ 13.01,P＜0.05)和Olive尾矩(t=12.44～14.88,P＜0.05)、尾部DNA含量(t=12.05 ～ 17.30,P＜0.05)均明显降低.两个高浓度防护组(1、2 mmol/L)分别与两个低浓度防护组(0.2、0.5 mmol/L)比较,Olive尾矩明显降低(t=5.67 ～ 16.56,P＜0.05).2 mmol/L防护组与其余各防护组相比,微核率和微核细胞率明显降低(=4.23～5.57,P ＜0.05).结论抗放利对辐射诱导人外周血淋巴细胞遗传损伤具有防护作用.
목적 탐토2,2-이갑기사경새서염산염(항방리)대복사유도인외주혈림파세포유전손상적방호작용.방법 채집건강인외주혈,분위건강대조조、단순조사조화항방리방호조.조사제량위2 Gy.항방리방호조분0.2、0.5、1、2 mmol/L 4개농도조,우조전30 min급약.통과염색체기변분석、미핵급단세포응효전영검측,관찰항방리대복사치인외주혈림파세포유전손상적방호작용.결과 각농도항방리방호조여단순조사조비교,염색체기변솔(t=5.34 ～25.48,P＜0.05)、미핵세포솔(t=5.18～29.44,P＜0.05)여미핵솔(=4.67～12.04,P＜0.05)、혜성세포미장(t=16.18～ 19.64,P＜0.05)、미구(t=11.79 ～ 13.01,P＜0.05)화Olive미구(t=12.44～14.88,P＜0.05)、미부DNA함량(t=12.05 ～ 17.30,P＜0.05)균명현강저.량개고농도방호조(1、2 mmol/L)분별여량개저농도방호조(0.2、0.5 mmol/L)비교,Olive미구명현강저(t=5.67 ～ 16.56,P＜0.05).2 mmol/L방호조여기여각방호조상비,미핵솔화미핵세포솔명현강저(=4.23～5.57,P ＜0.05).결론 항방리대복사유도인외주혈림파세포유전손상구유방호작용.
Objective To investigate the protective effect of 2,2-dimethylthiazolidine (KFL) on human peripheral blood lymphocytes in radiation-induced genetic damage.Methods Peripheral blood samples were collected from healthy volunteers and divided into normal control group,irradiation group and KFL protective groups with four different concentrations (0.2,0.5,1,2 mmol/L).KFL was administrated 30 min before 2 Gy irradiation.Chromosome aberration analysis,micronucleus test and single cell gel electrophoresis were used to observe the protective effects of KFL on the radiation-induced genetic damage in human peripheral blood lymphocytes.Results Compared with the irradiation group,the four KFL protection groups showed significant reduction in the chromosomal aberration frequencies (t =5.34-25.48,P ＜0.05),the micronucleus cell frequencies (t =5.18-29.44,P ＜ 0.05),the micronucleus frequencies (t =4.67-12.04,P ＜ 0.05),the tailleghth (t =16.18-19.64,P ＜ 0.05),the tailmoment (t =11.79-13.01,P ＜ 0.05),the olivetailmoment (t =12.44-14.88,P ＜ 0.05) and the tail DNA％ of comet cells (t =12.05-17.30,P ＜ 0.05).The olivetailmoment of the two high concentrations in protective groups(1,2 mmol/L) were significantly decreased compared with the other two KFL groups (0.2,0.5 mmol/L),respectively (t =5.67-16.56,P ＜ 0.05).Compared with other KFL groups,the micronucleus frequencies and micronucleus cell frequencies of the 2 mmol/L KFL group was significantly decreased (t =4.23-5.57,P ＜ 0.05).Conclusions KFL may have protective effect on human peripheral blood lymphocytes in the genetic damage induced by radiation.