CAJ | 학술논문

目的观察NADPH氧化酶抑制剂二甲苯基碘(DPI)对放射性肺损伤的防治作用,探讨其可能机制.方法 48只成年雄性SD大鼠按随机数字表法分为空白对照组(C组)、单纯照射组(R组)、照射+二甲苯基碘组(R+D组)和单用二甲苯基碘组(D组),每组12只.采用6MVX射线照射双肺,每只每天照射8 Gy,连续照射5d,总剂量40 Gy.R+D组大鼠在每次照射前1h皮下注射0.02％ DPI溶液(1 mg/kg);D组大鼠不接受射线照射,只注射DPI溶液.两组大鼠DPI注射从照射前3d开始,持续至照射后30 d.C组和R组大鼠则注射等体积的生理盐水.照射后1个月和6个月时提取肺组织,行HE染色、Masson染色、羟脯氨酸含量测定和TGF-β1免疫组织化学染色.结果在照射后1个月时,R组出现典型肺泡炎表现,羟脯氨酸含量为(0.69±0.05) μg/mg,TGF-β1阳性染色面积比为(39.97±0.90)％,R+D组羟脯氨酸含量为(0.55±0.03) μg/mg,TGF-β1阳性染色面积比为(33.83±1.55)％,肺泡炎程度轻于R组(t =5.32、5.93,P＜0.05).6个月时R组出现典型肺纤维化表现,羟脯氨酸含量为(1.04±0.02) μg/mg,TGF-β1阳性染色面积比为(37.80±0.85)％,R+D组羟脯氨酸含量为(0.85±0.02) μg/mg,TGF-β1阳性染色面积比为(23.93±1.16)％,肺纤维化程度轻于R组(t=15.77、16.68,P＜0.05).C组和D组则未发生明显异常变化.结论二甲苯基碘可以减少大鼠肺部照射后羟脯氨酸及TGF-β1的含量,减轻肺损伤的程度,对放射性肺损伤具有一定的防治作用
목적 관찰NADPH양화매억제제이갑분기전(DPI)대방사성폐손상적방치작용,탐토기가능궤제.방법 48지성년웅성SD대서안수궤수자표법분위공백대조조(C조)、단순조사조(R조)、조사+이갑분기전조(R+D조)화단용이갑분기전조(D조),매조12지.채용6MVX사선조사쌍폐,매지매천조사8 Gy,련속조사5d,총제량40 Gy.R+D조대서재매차조사전1h피하주사0.02％ DPI용액(1 mg/kg);D조대서불접수사선조사,지주사DPI용액.량조대서DPI주사종조사전3d개시,지속지조사후30 d.C조화R조대서칙주사등체적적생리염수.조사후1개월화6개월시제취폐조직,행HE염색、Masson염색、간포안산함량측정화TGF-β1면역조직화학염색.결과 재조사후1개월시,R조출현전형폐포염표현,간포안산함량위(0.69±0.05) μg/mg,TGF-β1양성염색면적비위(39.97±0.90)％,R+D조간포안산함량위(0.55±0.03) μg/mg,TGF-β1양성염색면적비위(33.83±1.55)％,폐포염정도경우R조(t =5.32、5.93,P＜0.05).6개월시R조출현전형폐섬유화표현,간포안산함량위(1.04±0.02) μg/mg,TGF-β1양성염색면적비위(37.80±0.85)％,R+D조간포안산함량위(0.85±0.02) μg/mg,TGF-β1양성염색면적비위(23.93±1.16)％,폐섬유화정도경우R조(t=15.77、16.68,P＜0.05).C조화D조칙미발생명현이상변화.결론 이갑분기전가이감소대서폐부조사후간포안산급TGF-β1적함량,감경폐손상적정도,대방사성폐손상구유일정적방치작용
Objective To investigate the mechanism of an inhibitor of NADPH oxidases,diphenylene iodonium (DPI),in preventing radiation-induced lung injury.Methods Totally 48 adult SD male rats were randomly classified into 4 groups:control group (C),radiation group (R),radiation plus DPI group (R + D) and DPI group (D).The radiation induced pulmonary injury model was preformed by using 6 MV X-rays to deliver 8 Gy per day for 5 consecutive days with 40 Gy in total to the thorax of each animal.Rats in R + D group were subcutaneously administered with 0.02％ DPI (1 mg/kg) at 1 h prior to radiation while rats in D group received the same dose of DPI without radiation.DPI was given from 3 d before radiation to 30 d after the first radiation.Rats in C and D groups received the same dose of saline.Animals were sacrificed at 1 month and 6 months after radiation,respectively.The lungs were removed and processed for HE and Masson staining,hydroxyproline content measurement,and TGF-β1 immunohistochemical detection.Results At 1 month post-radiation,rats in R group showed typical alveolitis,the level of hydroxyproline was (0.69 ± 0.05) μg/mg,and the positive area of TGF-β1 expression was (39.97 ± 0.90) ％,while the level of hydroxyproline in R + D group was (0.55 ± 0.03) μg/mg and the positive area of TGF-β1 expression was(33.83 ± 1.55) ％,rats in R + D group showed less severe alveolitis compared with R group(t =5.32,5.93,P ＜0.05).At 6 months post-radiation,rats in R group showed typical lung fibrosis with hydroxyproline level of (1.04 ±-0.02) μg/mg and TGF-β1 expression of (37.80 ± 0.85) ％,whereas the hydroxyproline level in R + D group was (0.85 ± 0.02) μg/ mg,the TGF-β1 expression was(23.93 ± 1.16)％,rats in R + D group showed moderate lung fibrosis(t =15.77,16.68,P ＜ 0.05),rats in C and D group had no noticeable changes.Conclusions Diphenylene iodonium could prevent radiation-induced lung injury by reducing the level of hydroxyproline and the expression of TGF-β1.