中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2014年
3期
172-175,187
,共5页
目的 观察γ射线对小鼠造血功能及外周血T细胞相关细胞因子和脾脏T细胞转录因子的影响,探讨射线引起的造血功能损伤与免疫异常的相关性.方法 将50只BALB/e小鼠用随机数字表法分为两组:照射组(40只)和空白对照组(10只),照射组小鼠予60Coγ射线(1.0 Gy/min,5.5 min)照射,空白对照组予假照射,照射组于照射后4、8、12及20 d,空白对照组于20 d,计数小鼠外周血白细胞和血小板;骨髓病理切片观察骨髓造血组织增生情况;流式细胞微球芯片捕获技术(CBA)检测外周血中Th 1/Th2/Th 17细胞因子含量变化;Western blot法检测脾脏Th1/Th2、Th17/Treg细胞分化特异性转录因子T-bet/GATA-3、RORγt/Foxp3蛋白表达水平.结果 照射组小鼠外周血白细胞和血小板计数较空白对照组显著降低(t白细胞=18.48、15.72、9.79、3.30,t血小板=22.52、19.74、11.78、4.70,P＜0.05).照射组骨髓病理切片与空白对照组相比,骨髓增生显著低下,造血组织面积减少,被脂肪组织大量替代,巨核细胞少见,照射后8d较20 d骨髓抑制更明显.照后8和12d,照射组小鼠外周血Th1类细胞因子IFN-γ、TNF-α及Th2类细胞因子IL-4、IL-6含量较空白对照组明显升高,IL-17A含量照射组较空白对照组亦明显升高(tIFN-γ=2.93、3.36,tTNF-α=6.09、8.11、6.43、4.49,tIL-4=4.49、3.18,tIL-6=5.11、8.67、6.67、8.55,P＜0.05).与空白对照组比较,照射组脾脏RORγt蛋白在照射后4、8和12d及GATA-3、Foxp3蛋白在各时间点表达水平均降低(tRORγt=6.79、4.31、4.47,tGATA-3=3.88、8.06、2.84,3.23,tFoxp3=10.00、8.06、2.89、5.93,P＜0.05).而照射组T-bet蛋白表达较空白对照组升高(t=5.64、2.75、3.56、4.65,P＜0.05).结论 5.5 Gyγ射线照射后引起小鼠骨髓造血抑制,同时导致Th 1/Th2、Th17/Treg细胞亚群功能失衡,提示由射线引起的免疫异常可能参与骨髓造血功能的损伤.
目的 觀察γ射線對小鼠造血功能及外週血T細胞相關細胞因子和脾髒T細胞轉錄因子的影響,探討射線引起的造血功能損傷與免疫異常的相關性.方法 將50隻BALB/e小鼠用隨機數字錶法分為兩組:照射組(40隻)和空白對照組(10隻),照射組小鼠予60Coγ射線(1.0 Gy/min,5.5 min)照射,空白對照組予假照射,照射組于照射後4、8、12及20 d,空白對照組于20 d,計數小鼠外週血白細胞和血小闆;骨髓病理切片觀察骨髓造血組織增生情況;流式細胞微毬芯片捕穫技術(CBA)檢測外週血中Th 1/Th2/Th 17細胞因子含量變化;Western blot法檢測脾髒Th1/Th2、Th17/Treg細胞分化特異性轉錄因子T-bet/GATA-3、RORγt/Foxp3蛋白錶達水平.結果 照射組小鼠外週血白細胞和血小闆計數較空白對照組顯著降低(t白細胞=18.48、15.72、9.79、3.30,t血小闆=22.52、19.74、11.78、4.70,P＜0.05).照射組骨髓病理切片與空白對照組相比,骨髓增生顯著低下,造血組織麵積減少,被脂肪組織大量替代,巨覈細胞少見,照射後8d較20 d骨髓抑製更明顯.照後8和12d,照射組小鼠外週血Th1類細胞因子IFN-γ、TNF-α及Th2類細胞因子IL-4、IL-6含量較空白對照組明顯升高,IL-17A含量照射組較空白對照組亦明顯升高(tIFN-γ=2.93、3.36,tTNF-α=6.09、8.11、6.43、4.49,tIL-4=4.49、3.18,tIL-6=5.11、8.67、6.67、8.55,P＜0.05).與空白對照組比較,照射組脾髒RORγt蛋白在照射後4、8和12d及GATA-3、Foxp3蛋白在各時間點錶達水平均降低(tRORγt=6.79、4.31、4.47,tGATA-3=3.88、8.06、2.84,3.23,tFoxp3=10.00、8.06、2.89、5.93,P＜0.05).而照射組T-bet蛋白錶達較空白對照組升高(t=5.64、2.75、3.56、4.65,P＜0.05).結論 5.5 Gyγ射線照射後引起小鼠骨髓造血抑製,同時導緻Th 1/Th2、Th17/Treg細胞亞群功能失衡,提示由射線引起的免疫異常可能參與骨髓造血功能的損傷.
목적 관찰γ사선대소서조혈공능급외주혈T세포상관세포인자화비장T세포전록인자적영향,탐토사선인기적조혈공능손상여면역이상적상관성.방법 장50지BALB/e소서용수궤수자표법분위량조:조사조(40지)화공백대조조(10지),조사조소서여60Coγ사선(1.0 Gy/min,5.5 min)조사,공백대조조여가조사,조사조우조사후4、8、12급20 d,공백대조조우20 d,계수소서외주혈백세포화혈소판;골수병리절편관찰골수조혈조직증생정황;류식세포미구심편포획기술(CBA)검측외주혈중Th 1/Th2/Th 17세포인자함량변화;Western blot법검측비장Th1/Th2、Th17/Treg세포분화특이성전록인자T-bet/GATA-3、RORγt/Foxp3단백표체수평.결과 조사조소서외주혈백세포화혈소판계수교공백대조조현저강저(t백세포=18.48、15.72、9.79、3.30,t혈소판=22.52、19.74、11.78、4.70,P＜0.05).조사조골수병리절편여공백대조조상비,골수증생현저저하,조혈조직면적감소,피지방조직대량체대,거핵세포소견,조사후8d교20 d골수억제경명현.조후8화12d,조사조소서외주혈Th1류세포인자IFN-γ、TNF-α급Th2류세포인자IL-4、IL-6함량교공백대조조명현승고,IL-17A함량조사조교공백대조조역명현승고(tIFN-γ=2.93、3.36,tTNF-α=6.09、8.11、6.43、4.49,tIL-4=4.49、3.18,tIL-6=5.11、8.67、6.67、8.55,P＜0.05).여공백대조조비교,조사조비장RORγt단백재조사후4、8화12d급GATA-3、Foxp3단백재각시간점표체수평균강저(tRORγt=6.79、4.31、4.47,tGATA-3=3.88、8.06、2.84,3.23,tFoxp3=10.00、8.06、2.89、5.93,P＜0.05).이조사조T-bet단백표체교공백대조조승고(t=5.64、2.75、3.56、4.65,P＜0.05).결론 5.5 Gyγ사선조사후인기소서골수조혈억제,동시도치Th 1/Th2、Th17/Treg세포아군공능실형,제시유사선인기적면역이상가능삼여골수조혈공능적손상.
Objective To observe the effects of whole body γ-ray radiation on hematopoiesis and cytokines related to T cell subsets in mouse,to detect the expression of transcription factors of splenic T cell subsets,and to investigate the correlation between hematopoiesis injury and abnormal immune function.Methods Totally 50 BALB/c mice were divided into radiation group and blank control group with the random number table method.The former group were given 5.5 Gy 60Co γ-ray radiation on whole body and another received sham radiation.The numbers of white blood cells and platelets of radiation group were counted at 4,8,12 and 20 d after radiation,and these numbers of blank control mice were counted only at 20 d.Hematopoietic tissue proliferation was evaluated by biopsy sections of mice femur.The contents of Th1,Th2,and Th17 in peripheral blood were detected with cytometric bead array (CBA).The expressions of T-bet/GATA-3 and RORγt/Foxp3 proteins related to the differentiation of T cell subsets in spleen tissue were measured by Western blot.Results The numbers of white blood cells and platelets of radiation group mice were reduced obviously (t WBC =18.48,15.72,9.79,3.30; t PLT =22.52,19.74,11.78,4.70,P ＜ 0.05) compared with blank control group.Biopsy sections showed that bone marrow hematopoietic cells of the radiated mice were less than those of blank group,and adipocytes became more.At 8 d,the marrow suppressions were more obvious than those at 20 d.Serum contents of Th1 cytokines IFN-γ,TNF-α and Th2 cytokines IL-4,IL-6 in the radiation group were higher than those in the blank control group at 8 and 12 d(t IFN-γ =2.93,3.36,t TNF-α =6.09,8.11,6.43,4.49,tIL-4 =4.49,3.18,t IL-6=5.11,8.67,6.67,8.55,P＜0.05).IL-17A secreted mainly by Th17 cells was also higher than the blank (t =3.68,6.24,5.32,4.06,P ＜ 0.05).Compared with the blank control group,the expression of T-bet protein increased significantly (t =5.64,2.75,3.56,4.65,P ＜ 0.05),and the expressions of GATA-3,RORγt,and Foxp3 proteins decreased at 4,8 and 12 d except the RORγt at 20 d (tRORγt =6.79,4.31,4.47,tGATA-3 =3.88,8.06,2.84,3.23,tFoxp3 =10.00,8.06,2.89,5.93,P＜ 0.05).Conclusions 5.5 Gy whole body γ-ray radiation inhibits bone marrow hematopoiesis of BALB/c mice and makes the differentiation and function of T cells to be abnormal,which may be associated with bone marrow hematopoiesis obstacle.
