中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2014年
4期
271-274
,共4页
常鹏宇%姜新%曲超%罗景华%蒋鑫萍%董丽华%崔爽%曲雅勤
常鵬宇%薑新%麯超%囉景華%蔣鑫萍%董麗華%崔爽%麯雅勤
상붕우%강신%곡초%라경화%장흠평%동려화%최상%곡아근
脂肪干细胞%放射性肠损伤%修复
脂肪榦細胞%放射性腸損傷%脩複
지방간세포%방사성장손상%수복
Adipose-derived stem cells%Radiation-induced intestinal injury%Restoration
目的 评价人源脂肪干细胞对放射性肠上皮损伤的修复作用.方法 选用成年雄性SD大鼠共40只,用随机数字表法从中选取34只给予腹部15 GyX射线照射.造模结束后,取17只立即给予P6代人源脂肪干细胞腹腔注射治疗,移植的细胞数目为5×106/只;17只仅腹腔照射的大鼠作为对照;剩余6只大鼠作为健康对照组.于照后10d,各组中抽签方法随机选取3只大鼠,并将其处死.取肠管进行HE染色、末端标记法(TUNEL)原位凋亡检测、Ki-67免疫组织化学染色以及Bmil免疫组化染色;提取组织总RNA,实时荧光定量PCR检测受照肠组织表皮生长因子(EGF)和角质细胞生长因子(KGF),上皮细胞标记E-cadherin,凋亡相关基因Bcl-2及BAX的表达量.分析各组大鼠的生存时间及生存率.结果 与单纯照射组相比,人源脂肪干细胞(ADSC)可促进受照肠上皮细胞的增殖(=18.30,P＜0.05),抑制上皮细胞凋亡(t=-32.48,P＜0.05),延长大鼠的生存时间(t=5.99,P ＜0.05),提高生存率.结论 人源脂肪于细胞对大鼠放射性肠上皮损伤具有修复作用.
目的 評價人源脂肪榦細胞對放射性腸上皮損傷的脩複作用.方法 選用成年雄性SD大鼠共40隻,用隨機數字錶法從中選取34隻給予腹部15 GyX射線照射.造模結束後,取17隻立即給予P6代人源脂肪榦細胞腹腔註射治療,移植的細胞數目為5×106/隻;17隻僅腹腔照射的大鼠作為對照;剩餘6隻大鼠作為健康對照組.于照後10d,各組中抽籤方法隨機選取3隻大鼠,併將其處死.取腸管進行HE染色、末耑標記法(TUNEL)原位凋亡檢測、Ki-67免疫組織化學染色以及Bmil免疫組化染色;提取組織總RNA,實時熒光定量PCR檢測受照腸組織錶皮生長因子(EGF)和角質細胞生長因子(KGF),上皮細胞標記E-cadherin,凋亡相關基因Bcl-2及BAX的錶達量.分析各組大鼠的生存時間及生存率.結果 與單純照射組相比,人源脂肪榦細胞(ADSC)可促進受照腸上皮細胞的增殖(=18.30,P＜0.05),抑製上皮細胞凋亡(t=-32.48,P＜0.05),延長大鼠的生存時間(t=5.99,P ＜0.05),提高生存率.結論 人源脂肪于細胞對大鼠放射性腸上皮損傷具有脩複作用.
목적 평개인원지방간세포대방사성장상피손상적수복작용.방법 선용성년웅성SD대서공40지,용수궤수자표법종중선취34지급여복부15 GyX사선조사.조모결속후,취17지립즉급여P6대인원지방간세포복강주사치료,이식적세포수목위5×106/지;17지부복강조사적대서작위대조;잉여6지대서작위건강대조조.우조후10d,각조중추첨방법수궤선취3지대서,병장기처사.취장관진행HE염색、말단표기법(TUNEL)원위조망검측、Ki-67면역조직화학염색이급Bmil면역조화염색;제취조직총RNA,실시형광정량PCR검측수조장조직표피생장인자(EGF)화각질세포생장인자(KGF),상피세포표기E-cadherin,조망상관기인Bcl-2급BAX적표체량.분석각조대서적생존시간급생존솔.결과 여단순조사조상비,인원지방간세포(ADSC)가촉진수조장상피세포적증식(=18.30,P＜0.05),억제상피세포조망(t=-32.48,P＜0.05),연장대서적생존시간(t=5.99,P ＜0.05),제고생존솔.결론 인원지방우세포대대서방사성장상피손상구유수복작용.
Objective To evaluate the therapeutic effect of human adipose-derived stem cells (ADSCs) on healing intestinal epithelium after abdominal irradiation.Methods A total of 40 SpragueDawley rats were irradiated to the whole abdomen with a single dose of 15 Gy.17 rats were randomly selected and infused intraperitoneally with 5 × 106 Passage 6 ADSCs,and 17 rats that received abdominal irradiation alone were set as control,6 rats were set as normal control.At 10 d post-irradiation,3 rats from each group were randomly selected and terminated.Freshly isolated small intestinal tissues were used for pathological analysis and EGF,KGF,E-cadherin,Bcl-2 and BAX mRNA levels analysis,and life-span and survival of rats were also analyzed.Results Compared to the control,infusion of ADSCs maintained the integrity of irradiated epithelium with more proliferative epithelial cells(t =18.30,P ＜ 0.05)and less apoptotic cells(t =-32.48,P ＜ 0.05).Besides,the rats receiving ADSCs-infusion survived longer than the control(t =5.99,P ＜ 0.05).Conclusions ADSCs can have therapeutic effect on healing radiationinduced intestinal epithelium injury.
