中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2014年
5期
334-337
,共4页
李轩%乔田奎%庄喜兵%张继红
李軒%喬田奎%莊喜兵%張繼紅
리헌%교전규%장희병%장계홍
含非甲基化二核苷酸的寡脱氧核苷酸1826%放射性肺纤维化%辐射防护
含非甲基化二覈苷痠的寡脫氧覈苷痠1826%放射性肺纖維化%輻射防護
함비갑기화이핵감산적과탈양핵감산1826%방사성폐섬유화%복사방호
Unmethylated cytosine-phosphate-guanine ligodeoxynucleotide (CpG ODN) 1826%Radiation pulmonary fibrosis%Radiation protection
目的 初步探讨含非甲基化二核苷酸的寡脱氧核苷酸(CpG ODN) 1826对大鼠放射性肺纤维化的防护作用.方法 采用6MVX射线单次左肺照射20 Gy,建立大鼠放射性肺纤维化模型.SD大鼠按完全随机法分为健康对照组、单纯照射组和药物干预组3组,每组30只.在照后0、1、3、5和7d,腹腔注射CpG ODN1826.照后5、15、30和90 d,取材,记录肺系数.照射肺石蜡切片HE染色、Masson染色,行肺纤维化评分.ELISA法检测血清TGF-β1浓度,碱化水解法检测肺组织羟脯氨酸(Hyp)浓度.结果 成功建立了放射性肺纤维大鼠模型.照后5d,健康对照组肺系数低于单纯照射组和药物干预组(t =3.046、2.252,P＜0.05).照后15、30和90 d,药物干预组肺系数低于单纯照射组(t=4.120、5.226和5.719,P＜0.05).单纯照射组及药物干预组照后30 d肺系数最高.药物干预组肺纤维化评分低于单纯照射组(t=3.212、4.959,P＜0.05).药物干预组血清TGF-β1浓度低于照射组(t=4.138、5.924、5.294和4.076,P＜0.05).单纯照射组照射肺Hyp含量高于药物干预组(t=7.527、8.416,P＜0.05).结论 CpG ODN1826可以降低放射性肺纤维化大鼠血清TGF-β1浓度及Hyp含量,预防放射性肺纤维化.
目的 初步探討含非甲基化二覈苷痠的寡脫氧覈苷痠(CpG ODN) 1826對大鼠放射性肺纖維化的防護作用.方法 採用6MVX射線單次左肺照射20 Gy,建立大鼠放射性肺纖維化模型.SD大鼠按完全隨機法分為健康對照組、單純照射組和藥物榦預組3組,每組30隻.在照後0、1、3、5和7d,腹腔註射CpG ODN1826.照後5、15、30和90 d,取材,記錄肺繫數.照射肺石蠟切片HE染色、Masson染色,行肺纖維化評分.ELISA法檢測血清TGF-β1濃度,堿化水解法檢測肺組織羥脯氨痠(Hyp)濃度.結果 成功建立瞭放射性肺纖維大鼠模型.照後5d,健康對照組肺繫數低于單純照射組和藥物榦預組(t =3.046、2.252,P＜0.05).照後15、30和90 d,藥物榦預組肺繫數低于單純照射組(t=4.120、5.226和5.719,P＜0.05).單純照射組及藥物榦預組照後30 d肺繫數最高.藥物榦預組肺纖維化評分低于單純照射組(t=3.212、4.959,P＜0.05).藥物榦預組血清TGF-β1濃度低于照射組(t=4.138、5.924、5.294和4.076,P＜0.05).單純照射組照射肺Hyp含量高于藥物榦預組(t=7.527、8.416,P＜0.05).結論 CpG ODN1826可以降低放射性肺纖維化大鼠血清TGF-β1濃度及Hyp含量,預防放射性肺纖維化.
목적 초보탐토함비갑기화이핵감산적과탈양핵감산(CpG ODN) 1826대대서방사성폐섬유화적방호작용.방법 채용6MVX사선단차좌폐조사20 Gy,건립대서방사성폐섬유화모형.SD대서안완전수궤법분위건강대조조、단순조사조화약물간예조3조,매조30지.재조후0、1、3、5화7d,복강주사CpG ODN1826.조후5、15、30화90 d,취재,기록폐계수.조사폐석사절편HE염색、Masson염색,행폐섬유화평분.ELISA법검측혈청TGF-β1농도,감화수해법검측폐조직간포안산(Hyp)농도.결과 성공건립료방사성폐섬유대서모형.조후5d,건강대조조폐계수저우단순조사조화약물간예조(t =3.046、2.252,P＜0.05).조후15、30화90 d,약물간예조폐계수저우단순조사조(t=4.120、5.226화5.719,P＜0.05).단순조사조급약물간예조조후30 d폐계수최고.약물간예조폐섬유화평분저우단순조사조(t=3.212、4.959,P＜0.05).약물간예조혈청TGF-β1농도저우조사조(t=4.138、5.924、5.294화4.076,P＜0.05).단순조사조조사폐Hyp함량고우약물간예조(t=7.527、8.416,P＜0.05).결론 CpG ODN1826가이강저방사성폐섬유화대서혈청TGF-β1농도급Hyp함량,예방방사성폐섬유화.
Objective To explore the protectional function of CpG ODN 1826 against radiation pulmonary fibrosis in rats.Methods The rat left lung was exposed to 20 Gy of 6 MV X-rays for establishing a radiation pulmonary fibrosis model.SD rats were randomly divided into control group,irradiated group and intervention group,with 30 rats in each group.CpG ODN 1826 was intraperitoneally injected into rats at 0,1,2,5 and 7 d post-irradiation.The rats were terminated at 5,15,30 and 90 d post-irradiation,and the lung indexes were recorded.Paraffin sections of the radiated lung were conducted with HE staining and Masson staining,the pulmonary fibrosis scores were recorded.The serum concentrations of TGF-β1 and hydroxyproline (Hyp) were measured.Results The radiation pulmonary fibrosis rat model was successfully established.The lung indexes of the control group were lower than those of the irradiated and intervention groups at 5 d post-irradiation (t =3.046,2.252,P ＜ 0.05).The lung indexes of the intervention group were lower than those of the irradiated group (t =4.120,5.226,5.719,P ＜ 0.05).Pulmonary fibrosis scores of intervention group were lower than those of irradiated group (t =3.212,4.959,P ＜ 0.05).The serum concentrations of TGF-β1 of irradiated group were higher than those of the intervention group (t =4.138,5.924,4.138,5.924,P ＜ 0.05).The Hyp in the lung of irradiated group was higher than that of intervention group (t =7.527,8.416,P ＜ 0.05).Conclusions CpG ODN1826 will not worse the radiation pulmonary fibrosis,on the contrary,it could reduce the serum concentrations of TGF-β1 and the lung content of Hyp in radiation pulmonary fibrosis,and protects rat against radiation pulmonary fibrosis.
