中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2014年
5期
342-344,375
,共4页
聂亮琴%周菊英%王利利%徐晓婷%秦颂兵
聶亮琴%週菊英%王利利%徐曉婷%秦頌兵
섭량금%주국영%왕리리%서효정%진송병
COX-2抑制剂%塞来昔布%放射增敏%脑胶质瘤细胞
COX-2抑製劑%塞來昔佈%放射增敏%腦膠質瘤細胞
COX-2억제제%새래석포%방사증민%뇌효질류세포
Cox-2 inhibitor%Celecoxib%Radiosensitivity%Glioblastoma
目的 探讨塞来昔布对正常少突胶质细胞(oln93)及脑胶质瘤细胞(u373)的放射增敏作用及其作用机制.方法 将两种细胞按空白处理、单独接受塞来昔布或X射线、塞来昔布联合X射线4种不同处理方式分为对照组、给药组、照射组和联合组,MTT法、克隆形成法对比两种细胞的增殖和放射敏感性,流式法测细胞的周期分布,Western blot法分析相关蛋白表达.结果 与未加药物相比,塞来昔布能抑制oln93细胞和u373细胞生长(t=2.215 ～ 30.996,P＜0.05;t =0.383～11.732,P＜0.05),但相同药物浓度下抑制两种细胞差异无统计学意义.放射增敏比SER分别为1.13和1.21,并诱导G0/G1期阻滞(t=-6.1 ～5.141,P＜0.05).联合组与照射组比较,oln93细胞发生S期阻滞(t=-18.174,P＜0.05),Cyclin A蛋白表达增加(t=-8.087,P＜0.05);u373细胞发生G2/M期阻滞,Cyclin B1、DNA-PKcs、MRE1 l蛋白表达下降(t=-8.838～10.45,P＜0.05).结论 塞来昔布对u373的放射增敏作用比oln93细胞明显,其机制与调节细胞周期分布及DNA损伤修复有关.
目的 探討塞來昔佈對正常少突膠質細胞(oln93)及腦膠質瘤細胞(u373)的放射增敏作用及其作用機製.方法 將兩種細胞按空白處理、單獨接受塞來昔佈或X射線、塞來昔佈聯閤X射線4種不同處理方式分為對照組、給藥組、照射組和聯閤組,MTT法、剋隆形成法對比兩種細胞的增殖和放射敏感性,流式法測細胞的週期分佈,Western blot法分析相關蛋白錶達.結果 與未加藥物相比,塞來昔佈能抑製oln93細胞和u373細胞生長(t=2.215 ～ 30.996,P＜0.05;t =0.383～11.732,P＜0.05),但相同藥物濃度下抑製兩種細胞差異無統計學意義.放射增敏比SER分彆為1.13和1.21,併誘導G0/G1期阻滯(t=-6.1 ～5.141,P＜0.05).聯閤組與照射組比較,oln93細胞髮生S期阻滯(t=-18.174,P＜0.05),Cyclin A蛋白錶達增加(t=-8.087,P＜0.05);u373細胞髮生G2/M期阻滯,Cyclin B1、DNA-PKcs、MRE1 l蛋白錶達下降(t=-8.838～10.45,P＜0.05).結論 塞來昔佈對u373的放射增敏作用比oln93細胞明顯,其機製與調節細胞週期分佈及DNA損傷脩複有關.
목적 탐토새래석포대정상소돌효질세포(oln93)급뇌효질류세포(u373)적방사증민작용급기작용궤제.방법 장량충세포안공백처리、단독접수새래석포혹X사선、새래석포연합X사선4충불동처리방식분위대조조、급약조、조사조화연합조,MTT법、극륭형성법대비량충세포적증식화방사민감성,류식법측세포적주기분포,Western blot법분석상관단백표체.결과 여미가약물상비,새래석포능억제oln93세포화u373세포생장(t=2.215 ～ 30.996,P＜0.05;t =0.383～11.732,P＜0.05),단상동약물농도하억제량충세포차이무통계학의의.방사증민비SER분별위1.13화1.21,병유도G0/G1기조체(t=-6.1 ～5.141,P＜0.05).연합조여조사조비교,oln93세포발생S기조체(t=-18.174,P＜0.05),Cyclin A단백표체증가(t=-8.087,P＜0.05);u373세포발생G2/M기조체,Cyclin B1、DNA-PKcs、MRE1 l단백표체하강(t=-8.838～10.45,P＜0.05).결론 새래석포대u373적방사증민작용비oln93세포명현,기궤제여조절세포주기분포급DNA손상수복유관.
Objective To compare the radiosensitivity effect of celecoxib on oln93 and u373 cells,and to explore the related mechanism.Methods Both oln93 cells and u373 cells were respectively divided into control group,drug group,radiation group and combined group when treated with celecoxib and irradiation.Cell survival ratio was evaluated by MTT assay and clogenic assay.Flow cytometry and Western blot assay were used to measure cell cycle and protein expression.Results Celecoxib had a similar effect on oln93 and u373 cells in enhancing the radiosensitivity (t =2.215-30.996,P ＜ 0.05 ; t =0.383-11.732,P＜0.05)and blocking cellcycle in G0/G1(t=-6.1-5.141,P＜0.05).Compared with the radiation group,the combined group showed S phase arrest(t =-18.174,P ＜ 0.05),and increase of Cyclin A protein (t =-8.087,P ＜ 0.05) in oln93 cells,and G2/M arrest and decrease of Cyclin B1 and DNA-PKcs and MRE11 protein (t =-8.838-10.45,P ＜ 0.05) in u373 cells.Conclusions Celecoxib illustrates a more sensitive radiosensitivity to u373 cells by regulating its cell cycle and DNA damage repair.
