中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2014年
6期
411-414
,共4页
LyGDI%非小细胞肺癌%A549细胞%辐射抗性
LyGDI%非小細胞肺癌%A549細胞%輻射抗性
LyGDI%비소세포폐암%A549세포%복사항성
LyGDI%Non-small-cell lung cancer%A549 cell%Radiation resistance
目的 探讨LyGDI的表达在非小细胞肺癌细胞A549中的辐射抗性机制.方法 A549和作为对照的H460细胞分别受到0、2、4和6 GyX射线照射后,克隆形成试验分析辐射抗性差异,Western blot分析LyGDI和辐射敏感性关键基因环氧合酶COX-2的表达.实时荧光定量PCR分析miR-34a以及miR-34b/c在A549和H460细胞中的表达.A549细胞中转染50 nmol/L的miR-34c成熟序列,观察其对A549细胞辐射抗性以及LyGDI和COX-2基因表达的影响.结果 LyGDI和COX-2在辐射抗性的A549细胞中的表达水平高于H460细胞,miR-34a以及miR-34b/c在两种非小细胞肺癌中低表达.转染miR-34c可抑制LyGDI、COX-2和Bcl-2以及激活p21的表达,增强A549细胞细胞的辐射敏感性(t=3.85、5.89、5.12,P＜0.05).结论 LyGDI诱导的COX-2的上调表达以及电离辐射效应miR-34家族的下调表达,是A549细胞辐射抗性的一个主要原因.
目的 探討LyGDI的錶達在非小細胞肺癌細胞A549中的輻射抗性機製.方法 A549和作為對照的H460細胞分彆受到0、2、4和6 GyX射線照射後,剋隆形成試驗分析輻射抗性差異,Western blot分析LyGDI和輻射敏感性關鍵基因環氧閤酶COX-2的錶達.實時熒光定量PCR分析miR-34a以及miR-34b/c在A549和H460細胞中的錶達.A549細胞中轉染50 nmol/L的miR-34c成熟序列,觀察其對A549細胞輻射抗性以及LyGDI和COX-2基因錶達的影響.結果 LyGDI和COX-2在輻射抗性的A549細胞中的錶達水平高于H460細胞,miR-34a以及miR-34b/c在兩種非小細胞肺癌中低錶達.轉染miR-34c可抑製LyGDI、COX-2和Bcl-2以及激活p21的錶達,增彊A549細胞細胞的輻射敏感性(t=3.85、5.89、5.12,P＜0.05).結論 LyGDI誘導的COX-2的上調錶達以及電離輻射效應miR-34傢族的下調錶達,是A549細胞輻射抗性的一箇主要原因.
목적 탐토LyGDI적표체재비소세포폐암세포A549중적복사항성궤제.방법 A549화작위대조적H460세포분별수도0、2、4화6 GyX사선조사후,극륭형성시험분석복사항성차이,Western blot분석LyGDI화복사민감성관건기인배양합매COX-2적표체.실시형광정량PCR분석miR-34a이급miR-34b/c재A549화H460세포중적표체.A549세포중전염50 nmol/L적miR-34c성숙서렬,관찰기대A549세포복사항성이급LyGDI화COX-2기인표체적영향.결과 LyGDI화COX-2재복사항성적A549세포중적표체수평고우H460세포,miR-34a이급miR-34b/c재량충비소세포폐암중저표체.전염miR-34c가억제LyGDI、COX-2화Bcl-2이급격활p21적표체,증강A549세포세포적복사민감성(t=3.85、5.89、5.12,P＜0.05).결론 LyGDI유도적COX-2적상조표체이급전리복사효응miR-34가족적하조표체,시A549세포복사항성적일개주요원인.
Objective To elucidate the mechanism of radiation resistant effect of LyGDI on NSCLC A549 cells.Methods A549 and H460 cells were irradiated with X-rays of 0,2,4 and 6 Gy.The clone-forming assay was used to detect cell survival and radiosensitivity.The expressions of LyGDI and COX-2 (Cyclooxygenase-2),a key radiosensitivity-related protein,were detected using Western blot.The miR-34 families were analyzed with RT-PCR.50 nmol/L mature miR-34c was transfected into A549 cells.Results The expression levels of LyGDI and COX-2 were much higher in radioresistive A549 cells than that in H460 cells.While the expression of miR-34a was quite low and miR-34b/c was hardly found in both NSCLC cells.Transfection of miR-34c into A549 cells strongly enhanced X-ray induced apoptosis by inhibiting the activations of LyGDI,COX-2,Bcl-2 and p21.Conclusions Up-regulation of LyGDI could induce COX-2 expression.The low expression of miR-34 family might be responsible for the radiation resistance of NSCLC cells.
