中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2014年
7期
489-492
,共4页
陈静%马林%曲宝林%李建雄%蓝玉玲%杨咏强%孟玲玲%冯林春
陳靜%馬林%麯寶林%李建雄%藍玉玲%楊詠彊%孟玲玲%馮林春
진정%마림%곡보림%리건웅%람옥령%양영강%맹령령%풍림춘
表皮生长因子受体%尼妥珠单抗%西妥昔单抗%食管癌%照射
錶皮生長因子受體%尼妥珠單抗%西妥昔單抗%食管癌%照射
표피생장인자수체%니타주단항%서타석단항%식관암%조사
Epidermal growth factor receptor%Nimotuzumab%Cetuximab%Esophageal carcinoma%Irradiation
目的观察尼妥珠单抗(h-R3)、西妥昔单抗(C225)联合照射对人食管鳞癌细胞系TE-13的作用.方法 分为无药对照组、h-R3组、C225组、单纯照射组、h-R3联合照射组、C225联合照射组,采用MTS法观察处理组对食管癌细胞增殖的影响,并计算细胞增殖率;采用克隆形成实验检测h-R3、C225对食管癌细胞系放射敏感性的影响,多靶单击模型拟合细胞存活曲线;通过流式细胞仪分析细胞周期分布及细胞凋亡变化.结果 与单纯照射组相比,单抗联合照射组的细胞增殖率明显降低(F=325.59,P ＜0.05),细胞凋亡率明显升高(F=120.59,P＜0.05),SF2、D0、Dq及N值均显著降低,G0/G1、G2/M期细胞比例均增加,S期细胞比例减少.C225联合照射组的细胞凋亡率高于h-R3联合照射组(F=120.59,P＜0.05),C225联合照射组SER(1.83)高于h-R3联合照射组SER(1.46).结论h-R3与C225均能提高照射对食管鳞癌细胞系TE-13的抗肿瘤作用,C225的杀伤作用略优于h-R3.
目的觀察尼妥珠單抗(h-R3)、西妥昔單抗(C225)聯閤照射對人食管鱗癌細胞繫TE-13的作用.方法 分為無藥對照組、h-R3組、C225組、單純照射組、h-R3聯閤照射組、C225聯閤照射組,採用MTS法觀察處理組對食管癌細胞增殖的影響,併計算細胞增殖率;採用剋隆形成實驗檢測h-R3、C225對食管癌細胞繫放射敏感性的影響,多靶單擊模型擬閤細胞存活麯線;通過流式細胞儀分析細胞週期分佈及細胞凋亡變化.結果 與單純照射組相比,單抗聯閤照射組的細胞增殖率明顯降低(F=325.59,P ＜0.05),細胞凋亡率明顯升高(F=120.59,P＜0.05),SF2、D0、Dq及N值均顯著降低,G0/G1、G2/M期細胞比例均增加,S期細胞比例減少.C225聯閤照射組的細胞凋亡率高于h-R3聯閤照射組(F=120.59,P＜0.05),C225聯閤照射組SER(1.83)高于h-R3聯閤照射組SER(1.46).結論h-R3與C225均能提高照射對食管鱗癌細胞繫TE-13的抗腫瘤作用,C225的殺傷作用略優于h-R3.
목적관찰니타주단항(h-R3)、서타석단항(C225)연합조사대인식관린암세포계TE-13적작용.방법 분위무약대조조、h-R3조、C225조、단순조사조、h-R3연합조사조、C225연합조사조,채용MTS법관찰처리조대식관암세포증식적영향,병계산세포증식솔;채용극륭형성실험검측h-R3、C225대식관암세포계방사민감성적영향,다파단격모형의합세포존활곡선;통과류식세포의분석세포주기분포급세포조망변화.결과 여단순조사조상비,단항연합조사조적세포증식솔명현강저(F=325.59,P ＜0.05),세포조망솔명현승고(F=120.59,P＜0.05),SF2、D0、Dq급N치균현저강저,G0/G1、G2/M기세포비례균증가,S기세포비례감소.C225연합조사조적세포조망솔고우h-R3연합조사조(F=120.59,P＜0.05),C225연합조사조SER(1.83)고우h-R3연합조사조SER(1.46).결론h-R3여C225균능제고조사대식관린암세포계TE-13적항종류작용,C225적살상작용략우우h-R3.
Objective To observe the combination effects of radiation and nimotuzumab (h-R3)or cetuximab (C225) on human esophageal squamous carcinoma cell line TE-13.Methods TE-13 cells were divided into 6 groups,including no treatment control,h-R3,C225,irradiation,h-R3 combined with irradiation,and C225 combined with irradiation.For each group,cell proliferation was evaluated by MTS assay,cell radiosensitivity was determined by clonogenic assay and the survival curve was fitted with the multi-targets single-hit model,and cell cycle distribution and apoptosis were analyzed with flow cytometry assay.Results Compared with the irradiation group,after the combination treatment of mAbs and irradiation,the cell proliferation,the cell proliferations were obviously decreased (F=325.59,P＜0.05),SF2,Do,Dq,and N values were obviously decreased,the cell apoptosis rates were significantly increased (F =120.59,P ＜ 0.05),and the percentages of cells in G0/G1 and G2/M phases were increased.The cell apoptosis rate of the C225 combined with irradiation group was higher than that of the h-R3 combined with irradiation group (F =120.59,P ＜ 0.05).The radiosensitization enhancement ratio (SER) of C225 was 1.83,higher than that of h-R3.Conclusions Both h-R3 and C225 can significantly enhance radiation damage of TE-13 cells and C225 is more effective than h-R3.
