CAJ | 학술논문

目的观察不同剂量富氢水对小鼠辐射损伤的保护作用,为富氢水的应用提供科学依据.方法 ICR小鼠按随机数字表法分为对照组、单纯照射组、氨磷汀组和富氢水低、中、高剂量组,经9.0 Gyγ射线全身照射后,观察各组动物30 d存活情况、体重和血液指标变化、存活动物的血清生化指标、脏器重量和系数、骨髓微核率、骨髓有核细胞计数等的变化.结果富氢水高剂量组在30 d存活率和照后体重方面表现出比较明显的保护作用并呈现一定的剂量效应关系,其中30 d平均存活天数跟单纯照射组比较,差异有统计学意义(t=-2.67,P＜0.05),富氢水组在血清生化指标方面也表现出一定的保护作用,其中,富氢水低剂量组总蛋白、白蛋白、肌酐,中剂量组总蛋白、白蛋白、总胆红素、肌酐,高剂量组总蛋白、白蛋白、血糖、总胆红素、尿素氮、肌酐、尿酸等指标跟单纯照射组比较差异有统计学意义(t=-2.04～-4.11,P＜0.05).富氢水组在血液学指标、脏器重量和系数、骨髓微核率等方面未观察到有明显保护作用.结论富氢水具有抗辐射损伤作用,而且存在一定的剂量效应关系.
목적 관찰불동제량부경수대소서복사손상적보호작용,위부경수적응용제공과학의거.방법 ICR소서안수궤수자표법분위대조조、단순조사조、안린정조화부경수저、중、고제량조,경9.0 Gyγ사선전신조사후,관찰각조동물30 d존활정황、체중화혈액지표변화、존활동물적혈청생화지표、장기중량화계수、골수미핵솔、골수유핵세포계수등적변화.결과 부경수고제량조재30 d존활솔화조후체중방면표현출비교명현적보호작용병정현일정적제량효응관계,기중30 d평균존활천수근단순조사조비교,차이유통계학의의(t=-2.67,P＜0.05),부경수조재혈청생화지표방면야표현출일정적보호작용,기중,부경수저제량조총단백、백단백、기항,중제량조총단백、백단백、총담홍소、기항,고제량조총단백、백단백、혈당、총담홍소、뇨소담、기항、뇨산등지표근단순조사조비교차이유통계학의의(t=-2.04～-4.11,P＜0.05).부경수조재혈액학지표、장기중량화계수、골수미핵솔등방면미관찰도유명현보호작용.결론 부경수구유항복사손상작용,이차존재일정적제량효응관계.
Objective To observe the protective effects of different doses of hydrogen-rich water on radiation injury in mice,so as to provide scientific basis for the application of hydrogen-rich water.Methods The ICR mice were randomly divided into control group,irradiation group,amifostine group and hydrogen-rich water of low,medium and high dose groups.The 30 days survival rate,body weight,hematology parameters,serum biochemical parameters,organ weight and coefficient,bone marrow micronucleus rate,bone marrow nucleated cell count were observed after total body irradiation with 9.0 Gy gamma rays.Results After 30 d of irradiation,the hydrogen-rich water showed obvious protective effect on the survival rate and body weight in a dose dependent manner so that the survival was significantly higher than that of irradiation group (t =-2.67,P ＜ 0.05).The biochemical index,such as TP,ALB and CRE in the low dose group,TP,ALB,TBIL and CRE in the medium dose group,and TP,ALB,GLU,TBIL,BUN,GRE and UA in the high dose group also indicated the protective effects of hydrogen-rich water (t =-2.04--4.11,P ＜ 0.05).But the protective effect of hydrogen-rich water was not observed in hematology,organ weight and coefficient,and bone marrow micronucleus induction.Conclusions The hydrogen-rich water has anti-radiation effect,which may depend on the dose of hydrogen.