中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2014年
10期
748-752
,共5页
王云莲%艾力江·吐尔逊%艾尼瓦尔·艾木都拉%肖蕾%张月芬%杨颖%吴戈%程纬%包永星
王雲蓮%艾力江·吐爾遜%艾尼瓦爾·艾木都拉%肖蕾%張月芬%楊穎%吳戈%程緯%包永星
왕운련%애력강·토이손%애니와이·애목도랍%초뢰%장월분%양영%오과%정위%포영성
结缔组织生长因子%转化生长因子β1%α-平滑肌肌动蛋白%肝纤维化%辐射
結締組織生長因子%轉化生長因子β1%α-平滑肌肌動蛋白%肝纖維化%輻射
결체조직생장인자%전화생장인자β1%α-평활기기동단백%간섬유화%복사
CTGT%TGF-β1%α-SMA%Hepatic fibrosis%Radiation
目的 探讨结缔组织生长因子(CTGF)在放射性肝纤维化大鼠中的动态表达及其与转化生长因子β1(TGF-β1)、α-平滑肌肌动蛋白(α-SMA)的相关性,了解CTGF与肝星状细胞(HSC)活化的关系.方法 雄性SD二级大鼠40只,用数字表法随机分为模型组(30只)和对照组(10只).模型组大鼠右半肝接受单次6MVX射线25 Gy照射,于照射后2、4和6个月,分别用数字表法随机抽取模型组大鼠10只,对照组予以假照射后6个月,均采用HE染色观察肝组织病理变化及大鼠肝纤维化半定量积分,免疫组织化学法、RT-PCR法检测肝组织中CTGF、TGF-β1、α-SMA及其mRNA表达,并对其进行相关性分析.结果 与对照组相比,模型组于照射后2、4和6个月,肝纤维化半定量积分逐渐增加(F=25.82,P＜0.05),模型组大鼠CTGF、TGF-β1、α-SMA蛋白的表达逐渐增多(F =55.44、16.22、121.12,P＜0.05).对照组肝组织中,CTGF、TGF-β1、α-SMA的mRNA仅有少量表达;模型组在照射2、4和6个月CTGF、TGF-β1、α-SMA的mRNA均逐渐升高(F=177.11、133.85、217.46,P＜0.05).相关性分析表明,大鼠肝组织中CTGF与α-SMA、TGF-β1与α-SMA均存在正相关(r =0.638、0.715,P＜0.05).结论 CTGF的表达随着肝纤维化程度的加重而升高,在放射性肝纤维化过程中CTGF可能参与了激活TGF-β信号通路,从而促进HSC的活化与增殖.
目的 探討結締組織生長因子(CTGF)在放射性肝纖維化大鼠中的動態錶達及其與轉化生長因子β1(TGF-β1)、α-平滑肌肌動蛋白(α-SMA)的相關性,瞭解CTGF與肝星狀細胞(HSC)活化的關繫.方法 雄性SD二級大鼠40隻,用數字錶法隨機分為模型組(30隻)和對照組(10隻).模型組大鼠右半肝接受單次6MVX射線25 Gy照射,于照射後2、4和6箇月,分彆用數字錶法隨機抽取模型組大鼠10隻,對照組予以假照射後6箇月,均採用HE染色觀察肝組織病理變化及大鼠肝纖維化半定量積分,免疫組織化學法、RT-PCR法檢測肝組織中CTGF、TGF-β1、α-SMA及其mRNA錶達,併對其進行相關性分析.結果 與對照組相比,模型組于照射後2、4和6箇月,肝纖維化半定量積分逐漸增加(F=25.82,P＜0.05),模型組大鼠CTGF、TGF-β1、α-SMA蛋白的錶達逐漸增多(F =55.44、16.22、121.12,P＜0.05).對照組肝組織中,CTGF、TGF-β1、α-SMA的mRNA僅有少量錶達;模型組在照射2、4和6箇月CTGF、TGF-β1、α-SMA的mRNA均逐漸升高(F=177.11、133.85、217.46,P＜0.05).相關性分析錶明,大鼠肝組織中CTGF與α-SMA、TGF-β1與α-SMA均存在正相關(r =0.638、0.715,P＜0.05).結論 CTGF的錶達隨著肝纖維化程度的加重而升高,在放射性肝纖維化過程中CTGF可能參與瞭激活TGF-β信號通路,從而促進HSC的活化與增殖.
목적 탐토결체조직생장인자(CTGF)재방사성간섬유화대서중적동태표체급기여전화생장인자β1(TGF-β1)、α-평활기기동단백(α-SMA)적상관성,료해CTGF여간성상세포(HSC)활화적관계.방법 웅성SD이급대서40지,용수자표법수궤분위모형조(30지)화대조조(10지).모형조대서우반간접수단차6MVX사선25 Gy조사,우조사후2、4화6개월,분별용수자표법수궤추취모형조대서10지,대조조여이가조사후6개월,균채용HE염색관찰간조직병리변화급대서간섬유화반정량적분,면역조직화학법、RT-PCR법검측간조직중CTGF、TGF-β1、α-SMA급기mRNA표체,병대기진행상관성분석.결과 여대조조상비,모형조우조사후2、4화6개월,간섬유화반정량적분축점증가(F=25.82,P＜0.05),모형조대서CTGF、TGF-β1、α-SMA단백적표체축점증다(F =55.44、16.22、121.12,P＜0.05).대조조간조직중,CTGF、TGF-β1、α-SMA적mRNA부유소량표체;모형조재조사2、4화6개월CTGF、TGF-β1、α-SMA적mRNA균축점승고(F=177.11、133.85、217.46,P＜0.05).상관성분석표명,대서간조직중CTGF여α-SMA、TGF-β1여α-SMA균존재정상관(r =0.638、0.715,P＜0.05).결론 CTGF적표체수착간섬유화정도적가중이승고,재방사성간섬유화과정중CTGF가능삼여료격활TGF-β신호통로,종이촉진HSC적활화여증식.
Objective To study the expression of connective tissue growth factor (CTGF) in radiation-induced hepatic fibrosis and explore its correlation with the expression of transforming growth factor β1 (TGF-β1) and α-smooth muscle actin (α-SMA) as well as the activation of hepatic stellate cells (HSC).Methods Forty healthy male Sprague-Dawley rats were randomly divided into model group (30 rats) and control group (10 rats).The right-half liver of rat was irradiated with a single dose of 25 Gy to establish a radiation-induced hepatic fibrosis model.Liver tissues of irradiated rats were collected at 2,4,and 6 months after irradiation.Pathomorphological changes in these liver tissues were stained with hematoxylin-eosin and observed with an optical microscope.The mRNA and protein expressions of CTGF,TGF-β1 and α-SMA were assayed by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry.Results The longer the irradiation time,the degree of liver fibrosis was more serious.The expressions of CTGF,TGF-β1,α-SMA protein in the model group increased along with the time after irradiation (F =55.44,16.22,121.12,P ＜0.05).The mRNA expressions of CTGF,TGF-β1 and α-SMA in the model group had positive correlations (r =0.638,0.715,P ＜ 0.05) and were significantly higher than the control (F =177.11,133.85,217.46,P ＜0.05).Conclusions The expression of CTGF had a positive relationship with the development of hepatic fibrosis.CTGF may be involved in the activation of TGF-β signaling pathway and thereby promotes the activation and proliferation of HSC.
