CAJ | 학술논문

目的探讨氨基肽酶N(CD13)在肝细胞癌中的表达水平及其临床意义.方法应用免疫组织化学染色方法检测CD13在55例肝癌组织及其相应癌旁组织和13例正常肝组织中的表达水平.运用X2检验分析肿瘤组织中CD13表达与临床病理因素的关系.运用Kaplan-Meier法分析肿瘤组织CD13与患者术后总生存时间的关系.结果 CD13在肝癌组织中的阳性表达率为72.73％(40/55),在癌旁组织的阳性表达率为21.81％(12/55),在正常肝组织的阳性表达率为7.69％(1/13).与癌旁组织和正常组织比较,肝癌组织中CDI3的阳性表达率显著升高,差异有统计学意义(P＜0.05).进一步分析显示CD13表达与肝癌的分化程度、包膜侵犯、淋巴结转移、门静脉癌栓形成、临床TNM分期等临床病理因素相关(P＜0.05).K-M生存曲线提示肿瘤CD13阳性表达的病例较阴性表达者生存率降低,差异具有统计学意义(P＜0.05).多因素COX逐步回归分析提示CDI3是影响总体生存率预后的独立危险因素.结论 CD13在肝癌的发生和发展中可能发挥重要的作用,可作为肝癌临床诊断和判断预后的一项重要指标.
목적 탐토안기태매N(CD13)재간세포암중적표체수평급기림상의의.방법 응용면역조직화학염색방법검측CD13재55례간암조직급기상응암방조직화13례정상간조직중적표체수평.운용X2검험분석종류조직중CD13표체여림상병리인소적관계.운용Kaplan-Meier법분석종류조직CD13여환자술후총생존시간적관계.결과 CD13재간암조직중적양성표체솔위72.73％(40/55),재암방조직적양성표체솔위21.81％(12/55),재정상간조직적양성표체솔위7.69％(1/13).여암방조직화정상조직비교,간암조직중CDI3적양성표체솔현저승고,차이유통계학의의(P＜0.05).진일보분석현시CD13표체여간암적분화정도、포막침범、림파결전이、문정맥암전형성、림상TNM분기등림상병리인소상관(P＜0.05).K-M생존곡선제시종류CD13양성표체적병례교음성표체자생존솔강저,차이구유통계학의의(P＜0.05).다인소COX축보회귀분석제시CDI3시영향총체생존솔예후적독립위험인소.결론 CD13재간암적발생화발전중가능발휘중요적작용,가작위간암림상진단화판단예후적일항중요지표.
Objective To explore the expression and clinical significance of Aminopeptidase N (CD13) in hepatocellular carcinoma (HCC).Methods The CD13 expressions from 55 cases of hepatocellular carcinoma with corresponding paracancerous tissue and 13 cases of normal liver tissue specimens were detected by immunohistochemical staining.The correlations of CD13 expression and clinicopathological characteristics were analyzed by Chi-squared test and survival curves were generated according to the Kaplan Meier method.Results The positive rate of CD13 protein was 72.73％ (40/55) in hepatocellular carcinoma tissue,21.81％ (12/55) in adjacent non-tumor tissue and 7.69 ％ (1/13) in normal liver tissue.The expression of CD13 was up-regulated significantly in HCC with statistically significant differences (P＜0.05).Statistical analysis revealed that CD13 expression was correlated with histological differentiation,capsule invasion,portal vein tumor thrombus,lymph node metastasis and clinical stages in HCC.The survival curves showed that the survival rate was significantly lower in patients with positive CD13 expression compared to negative expression (P＜0.05).Also,the mul tivariate COX proportional hazard model showed that CD13 expression was an independent predictor of overall survival (P＜0.05).Conclusion Therefore,CD13 plays a mutually promoting role in the progression and development of HCC and may be a diagnostic and prognostic indicator for HCC patients.