CAJ | 학술논문

目的制备VEGF靶向荷钆纳米探针,并研究其在小肝癌特异性成像中的价值.方法采用生物相容性高分子材料聚乳酸-聚乙二醇聚赖氨酸(PLA-PEG-PLL)纳米粒为载体,分别将血管内皮生长因子(VEGF)抗体及钆基接合至载体表面得到靶向纳米探针.体外MR成像测定其驰豫率,再利用种植型H22肝癌小鼠模型进行靶向纳米探针MR增强扫描,探讨皮下种植肝癌及微小病灶的强化表现特点.结果制备的靶向探针粒径和Zata电位分别为(85.8±7.2)nm和(21.63±2.4)mV,当其浓度为8.0 μmol/ml时测得的R1值为18.394 mmol· ml-1·s-1.靶向探针MR增强扫描显示皮下较大肝癌及小肝癌均表现为明显的特异性靶向延迟期增强,强化峰值分别在2h及3h时出现,而非靶向纳米粒增强扫描时肿瘤强化不明显.结论成功制备了合适的高驰豫率VEGF靶向PLA-PEG-PLL荷钆纳米探针,并实现了小肝癌的特异性靶向成像.该纳米探针在肝癌检测方面具有较好的临床应用前景.
목적 제비VEGF파향하구납미탐침,병연구기재소간암특이성성상중적개치.방법 채용생물상용성고분자재료취유산-취을이순취뢰안산(PLA-PEG-PLL)납미립위재체,분별장혈관내피생장인자(VEGF)항체급구기접합지재체표면득도파향납미탐침.체외MR성상측정기치예솔,재이용충식형H22간암소서모형진행파향납미탐침MR증강소묘,탐토피하충식간암급미소병조적강화표현특점.결과 제비적파향탐침립경화Zata전위분별위(85.8±7.2)nm화(21.63±2.4)mV,당기농도위8.0 μmol/ml시측득적R1치위18.394 mmol· ml-1·s-1.파향탐침MR증강소묘현시피하교대간암급소간암균표현위명현적특이성파향연지기증강,강화봉치분별재2h급3h시출현,이비파향납미립증강소묘시종류강화불명현.결론 성공제비료합괄적고치예솔VEGF파향PLA-PEG-PLL하구납미탐침,병실현료소간암적특이성파향성상.해납미탐침재간암검측방면구유교호적림상응용전경.
Objective This study aims to synthesize a novel gadolinium loaded nanoprobe targeted to vascular endothelial growth factor (VEGF) and assess its clinical value for imaging micro hepatic carcinoma.Methods A carrier was made by the biocompatible polymer material polylactic acid-polyethylene glycol-poly-L-lysinenanoparticles (PLA-PEG-PLLs).The targeted nanoprobe was obtained with anti-VEGF antibody and gadolinium (Gd) bonding to the surface of the carrier.MRI in vitro determined the T1 relaxivity of the nanoprobe.A live cancer model enhanced MR scan was performed by injecting targeted nanoprobes into the tail vein of grafted H22 tumor mice.The enhanced characteristics of the subcutaneous tumors and micro-heatic carcinoma were then reviewed.Results The particle size of the VEGF-targeted PLA-PEG-PLL gadolinium loaded nanoprobe was 85.8±7.2 nm with a zeta potential of 21.63±2.4 mV.The R1 relaxivity of the targeted nanoprobe was 18.394 mmol/s at 3.0 T when its gadolinium concentration was 8.0 μmol/ml.The enhanced MR scan using targeted probes showed that the big and micro-subcutaneous cancer exhibited a specifically delayed enhancement with an enhanced peak value at 2 or 3 hours,rather than the enhancement of the tumor using the nontargeted nanoparticles.Conclusion In conclusion,the VEGF targeted PLA-PEG-PLL gadolinium loaded nanoprobe was synthesized successfully,showed a high relaxivity,achieved targeted imaging of the micro-hepatic carcinoma,and exhibits a promising potential in the detection of this liver cancer.