中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2013年
3期
224-229
,共6页
薛立新%李绪雄%舒斌%杨春福%李可洲%田伏洲
薛立新%李緒雄%舒斌%楊春福%李可洲%田伏洲
설립신%리서웅%서빈%양춘복%리가주%전복주
胆管肿瘤%细胞增殖%细胞凋亡%环靶明
膽管腫瘤%細胞增殖%細胞凋亡%環靶明
담관종류%세포증식%세포조망%배파명
Bile duct neoplasms%Cell proliferation%Apoptosis%Cyclopamine
目的 研究Hedgehog信号通路特异性阻断剂环靶明(cyclopamine)对人胆管癌细胞系QBC939增殖与凋亡的影响.方法 用MTT比色法检测环靶明对QBC939细胞增殖的抑制作用,流式细胞术检测细胞凋亡率.RT-PCR法分别检测环靶明处理前后PTCH1、GLI1、EGFR在QBC939中的mRNA表达及变化.Western blot检测环靶明处理前后PTCH1、GLI1、EGFR在QBC939中的蛋白表达及变化.结果 环靶明抑制QBC939细胞的增殖,其作用呈剂量和时间依赖性.经5、10、20 μmol/L的环靶明作用48 h后,QBC939细胞的凋亡率逐渐升高,明显高于对照组的凋亡率(P＜0.01).PTCH1、GLI1、EGFR的mRNA和蛋白均在QBC939中表达,环靶明下调QBC939的PTCH1、GLI1、EGFR表达.结论 阻断Hedgehog信号通路能抑制胆管癌细胞QBC939的增殖,促进其凋亡.
目的 研究Hedgehog信號通路特異性阻斷劑環靶明(cyclopamine)對人膽管癌細胞繫QBC939增殖與凋亡的影響.方法 用MTT比色法檢測環靶明對QBC939細胞增殖的抑製作用,流式細胞術檢測細胞凋亡率.RT-PCR法分彆檢測環靶明處理前後PTCH1、GLI1、EGFR在QBC939中的mRNA錶達及變化.Western blot檢測環靶明處理前後PTCH1、GLI1、EGFR在QBC939中的蛋白錶達及變化.結果 環靶明抑製QBC939細胞的增殖,其作用呈劑量和時間依賴性.經5、10、20 μmol/L的環靶明作用48 h後,QBC939細胞的凋亡率逐漸升高,明顯高于對照組的凋亡率(P＜0.01).PTCH1、GLI1、EGFR的mRNA和蛋白均在QBC939中錶達,環靶明下調QBC939的PTCH1、GLI1、EGFR錶達.結論 阻斷Hedgehog信號通路能抑製膽管癌細胞QBC939的增殖,促進其凋亡.
목적 연구Hedgehog신호통로특이성조단제배파명(cyclopamine)대인담관암세포계QBC939증식여조망적영향.방법 용MTT비색법검측배파명대QBC939세포증식적억제작용,류식세포술검측세포조망솔.RT-PCR법분별검측배파명처리전후PTCH1、GLI1、EGFR재QBC939중적mRNA표체급변화.Western blot검측배파명처리전후PTCH1、GLI1、EGFR재QBC939중적단백표체급변화.결과 배파명억제QBC939세포적증식,기작용정제량화시간의뢰성.경5、10、20 μmol/L적배파명작용48 h후,QBC939세포적조망솔축점승고,명현고우대조조적조망솔(P＜0.01).PTCH1、GLI1、EGFR적mRNA화단백균재QBC939중표체,배파명하조QBC939적PTCH1、GLI1、EGFR표체.결론 조단Hedgehog신호통로능억제담관암세포QBC939적증식,촉진기조망.
Objective This article aims to study the impact of cyclopamine,a Hedgehog signaling pathway inhibitor,on the proliferation and apoptosis of QBC939 cholangiocarcinoma cells.Methods The proliferation of QBC939 cells was detected with the MTT assay,and the apoptotic rate was analyzed with the flow cytometry assay.RT-PCR and Western blow were used to detect the expressions of tumor-related genes and proteins in QBC939 cells before and after cyclopamine treatment.Results Our results show that cyclopamine inhibited the growth of QBC939 cells in time and dose dependent manners.After a 5,10,or 20 μmol/L cyclopamine treatment for 48 hours,QBC939 cells showed increased apoptotic rates significantly higher than those in the control group (P＜0.01).Furthermore,cyclopamine down regulated the mRNA and protein levels of PTCH1,GLI1,and EGFR in QBC939 cells.Conclusion Therefore,blockage of the Hedgehog signaling pathway with cyclopamine could suppress the proliferation and promote the apoptosis of QBC939 cholangiocarcinoma cells.
