CAJ | 학술논문

目的探讨原发性肝细胞癌切除术中快速灭活污染器械肿瘤细胞的方法.方法用37℃生理盐水浸泡并刷洗原发性肝细胞癌切除术中可疑污染器械.浸泡液经低速离心后,提取并培养沉淀的肿瘤细胞.使用不同温度的蒸馏水或生理盐水浸泡肿瘤细胞不同时间后,HE染色倒置显微镜下观察细胞的形态.结果在55℃蒸馏水中浸泡60s后,肿瘤细胞肿胀,胞膜消失,细胞核体积缩小,核内染色质浓缩,染色变深,部分细胞裂解脱落.肿瘤细胞经55℃蒸馏水浸泡60s后更换含胎牛血清(FBS)的RPMI 1640培养液再培养24 h,未发现贴壁生长的有活性细胞.而在55℃的生理盐水中浸泡60 s后,肿瘤细胞核膜形态未见明显改变.结论原发性肝细胞癌切除术中可疑污染器械在55℃蒸馏水中浸泡60 s,肿瘤细胞能被全部灭活.
목적 탐토원발성간세포암절제술중쾌속멸활오염기계종류세포적방법.방법 용37℃생리염수침포병쇄세원발성간세포암절제술중가의오염기계.침포액경저속리심후,제취병배양침정적종류세포.사용불동온도적증류수혹생리염수침포종류세포불동시간후,HE염색도치현미경하관찰세포적형태.결과 재55℃증류수중침포60s후,종류세포종창,포막소실,세포핵체적축소,핵내염색질농축,염색변심,부분세포렬해탈락.종류세포경55℃증류수침포60s후경환함태우혈청(FBS)적RPMI 1640배양액재배양24 h,미발현첩벽생장적유활성세포.이재55℃적생리염수중침포60 s후,종류세포핵막형태미견명현개변.결론 원발성간세포암절제술중가의오염기계재55℃증류수중침포60 s,종류세포능피전부멸활.
Objective This study investigated a method to rapidly inactivate tumor cells on surgical instruments intraoperatively.Methods Tumor cells were collected by immersing and washing the surgical instruments in 37 ℃ saline.The precipitation was collected by low speed centrifugation and then was cultured to harvest the tumor cells.The tumor cells were immersed in saline and distilled water of different temperatures for different duration of time.Inverted microscopy was used to investigate the changes in morphology.Results After immersion in 55 ℃ distilled water for 60 seconds,the tumor cells were swollen,the cell membranes disappeared,the sizes of the nuclei were reduced,the chromatin was condensed,and some cells lysed and separated from each other.Additionally,these tumor cells floated in the culture medium and lacked any living cells adhering to the walls of the bottle.In the group of tumor cells treated with 55 ℃ saline for 60 seconds,there were no obvious morphological changes of the tumor cell or nucleus.Conclusion The intraoperative immersion of surgical instruments in 55 ℃ distilled water for 60 seconds could completely inactivate tumor cells.