CAJ | 학술논문

目的探讨肝硬化环境对肝癌肝内、外转移的影响.方法健康雄性BALB/c小鼠,采用CCl4诱导建立肝硬化小鼠模型并设正常对照组.分别在肝硬化组及对照组的肝脏左叶上原位种植小鼠肝癌细胞,术后1、2、3周收取标本.通过大体及病理切片观察肿瘤的生长及转移行为.检测了肝脏组织中血管细胞黏附分子-1(VCAM-1)、E-选择素(E-selectin)和E-钙黏附素(E-cadherin)的表达情况.结果术后观察发现,肝硬化基础上的肝癌更容易出现肝内转移(14/19)与远处转移(4/19),与对照组相比(5/17,1/17)差异具有统计学意义(P＜0.05).同时肝硬化组黏附类分子较对照组表达明显上调(P＜0.01).钙黏附素表达则无明显差异.结论肝硬化环境更利于肝癌发生肝内、外转移.这种转移行为可能与肝硬化组织中各种黏附因子表达增高有关.
목적 탐토간경화배경대간암간내、외전이적영향.방법 건강웅성BALB/c소서,채용CCl4유도건립간경화소서모형병설정상대조조.분별재간경화조급대조조적간장좌협상원위충식소서간암세포,술후1、2、3주수취표본.통과대체급병리절편관찰종류적생장급전이행위.검측료간장조직중혈관세포점부분자-1(VCAM-1)、E-선택소(E-selectin)화E-개점부소(E-cadherin)적표체정황.결과 술후관찰발현,간경화기출상적간암경용역출현간내전이(14/19)여원처전이(4/19),여대조조상비(5/17,1/17)차이구유통계학의의(P＜0.05).동시간경화조점부류분자교대조조표체명현상조(P＜0.01).개점부소표체칙무명현차이.결론 간경화배경경리우간암발생간내、외전이.저충전이행위가능여간경화조직중각충점부인자표체증고유관.
Objective To study the effects of a cirrhotic environment on the metastasis of liver cancer in mice.Methods Male BALB/c mice were randomly assigned to two groups:cirrhosis group and control group.The cirrhosis group was treated by an injection of carbon tetrachloride intraperitoneally.H22 liver cancer cells were directly implanted under the capsule of each group after cirrhosis was established.The animals were sacrificed at 1,2,and 3 weeks after the operation.The metastatic behavior of the cancer cells was observed by the naked eye and microscopically.Additionally,the adhesion ability of the liver was assessed by measuring the expression of vascular cell adhesion molecule 1 (VCAM-1),E-selectin,and E-cadherin.Results After the operation,the cirrhosis group showed an obvious metastatic tendency in both intrahepatic (14/19) and extrahepatic ways (4/19),compared with the control group's intrahepatic (5/17) and extrahepatic ways (1/17) (P＜0.05).Immunohistochemisty for VCAM-1 and E-selectin showed a significant increase in the cirrhosis (P＜0.05).However,there was no difference observed in the E-cadherin between the cirrhosis and control group.Conclusion A cirrhotic liver environment may promote the metastasis of liver cancer cells by increasing the ability of liver adhesion.