CAJ | 학술논문

目的探讨组蛋白去甲基化酶PHD锌指蛋白8(PHF8)在肝细胞癌(HCC)中的表达及其与肝癌临床病理学特征和预后的关系.方法采用免疫组织化学SABC方法检测60例肝细胞癌患者肿瘤组织、相应癌旁组织以及15例正常肝组织中PHF8蛋白的表达,分析PHF8表达情况与肝癌各临床病理特征的关系.结果肝细胞癌中的PHF8表达阳性率为55.0％,明显高于相应癌旁组织及正常肝组织(阳性率分别为16.7％、6.7％)(P＜0.05).PHF8表达与肝细胞癌的肿瘤结节大小、数目、分化程度以及TNM分期密切相关(P＜0.05).PHF8阳性组5年无瘤生存率及总生存率均明显低于PHF8阴性组(P＜0.05).结论 PHF8表达上调在肝细胞癌的发生发展中起重要作用,与患者的预后密切相关,可为预测肝癌患者手术预后和复发提供重要的参考.
목적 탐토조단백거갑기화매PHD자지단백8(PHF8)재간세포암(HCC)중적표체급기여간암림상병이학특정화예후적관계.방법 채용면역조직화학SABC방법검측60례간세포암환자종류조직、상응암방조직이급15례정상간조직중PHF8단백적표체,분석PHF8표체정황여간암각림상병리특정적관계.결과 간세포암중적PHF8표체양성솔위55.0％,명현고우상응암방조직급정상간조직(양성솔분별위16.7％、6.7％)(P＜0.05).PHF8표체여간세포암적종류결절대소、수목、분화정도이급TNM분기밀절상관(P＜0.05).PHF8양성조5년무류생존솔급총생존솔균명현저우PHF8음성조(P＜0.05).결론 PHF8표체상조재간세포암적발생발전중기중요작용,여환자적예후밀절상관,가위예측간암환자수술예후화복발제공중요적삼고.
Objective To investigate the expression of PHF8(PHD-finger protein 8) in human hepatocellular carcinoma (HCC) and its clinicopathologic significance.Methods The expression of PHF8 in 60 hepatocellular carcinoma samples as well as their natched paraneoplastic tissues,and 15 normal liver tissues were evaluated by immunohistochemistry.Statistical methods were used to analyse the relationship between the expression of PHF8 and the clinicopathological features of these patients.Results The PHF8-positive expression rate in the HCC samples was 55.0％ and it was significantly higher than that in the paraneoplastic tissues and the normal liver tissucs (16.7％,6.7％,respectively,P＜0.05).The expression of PHF8 was closely related to tumor size,tumor nodular numbers,pathological differentiation and TNM-staging (P＜0.05 for all).The 5-year disease-free survival and overall survival in the PHF8-positive group was significantly lower than that in the negative group (P＜0.05).Conclusions PHF8 was overexpressed in HCC samples,and its expression was closely associated with HCC clinicopathological features and prognosis of the patients.