CAJ | 학술논문

目的探讨PIWIL1蛋白与DICER酶在肝细胞癌组织中的表达及临床意义.方法免疫组化检测47例肝细胞癌标本中PIWIL1与DICER的表达.蛋白印迹法检测31例肝细胞癌组织中PIWIL1与DICER的表达.分析PIWIL1与DICER表达及与临床特征的关系.结果 PIWIL1在癌组织中高表达,在正常肝组织中低表达(P＜0.05).DICER在正常肝组织中高表达,在癌组织中低表达或缺失(P＜0.05).PIWIL1和DICER与肿瘤分化程度、临近组织侵犯相关(P＜0.05),PIWIL1与DICER在组织中的表达呈负相关(P＜0.05).结论 PIWIL1的高表达和DICER的低表达或缺失与病理分化程度及临近组织侵犯相关,PIWIL1与DICER的表达负相关.
목적 탐토PIWIL1단백여DICER매재간세포암조직중적표체급림상의의.방법 면역조화검측47례간세포암표본중PIWIL1여DICER적표체.단백인적법검측31례간세포암조직중PIWIL1여DICER적표체.분석PIWIL1여DICER표체급여림상특정적관계.결과 PIWIL1재암조직중고표체,재정상간조직중저표체(P＜0.05).DICER재정상간조직중고표체,재암조직중저표체혹결실(P＜0.05).PIWIL1화DICER여종류분화정도、림근조직침범상관(P＜0.05),PIWIL1여DICER재조직중적표체정부상관(P＜0.05).결론 PIWIL1적고표체화DICER적저표체혹결실여병리분화정도급림근조직침범상관,PIWIL1여DICER적표체부상관.
Objective To explore the expression of PIWIL1 protein and DICER enzyme in hepa tocellular carcinoma (HCC) and their significance.Methods Immunohistochemical method was used to detect the expression of PIWIL1 and DICER in 47 cases of HCC and the adjacent HCC tissues.Western blot method was used to detect the expression of PIWIL1 and DICER in 31 cases of fresh HCC tissues and their adjacent HCC tissues.The relationship between PIWIL1 and DICER and their relationships were analysed with clinical features.12 cases of normal liver tissues were used as control group.Results The expression of PIWIL1 was high in HCC but low in normal liver tissues (P＜ 0.05).The expression of DICER was high in normal liver tissues but low in HCC (P＜0.05).The expression of PIWIL1 was positively correlated with invasion to adjacent tissues and histological differentiation (P＜0.05).The expression of DICER was negatively correlated with invasion to the adjacent tissues and histological differentiation (P＜0.05).There was a negative correlation between PIWIL1 and DICER (P＜ 0.05).Conclusions High expression of PIWIL1 and low/missing expression of DICER was related to pathological differentiation and invasion of adjacent tissues.