CAJ | 학술논문

目的探讨人脐血单个核细胞(haman cord blood mononuclear cells,HCMNCs)与大鼠雪旺细胞(Schwann cells,SCs)联合移植应用于大鼠脊髓损伤的疗效.方法健康成年8周龄Wistar雌性大鼠40只,体重(200±30)g.利用Impactor Model Ⅱ型打击器制成T10脊髓损伤模型.40只大鼠随机分成4组,每组10只,即DMEM实验对照组、HCMNCs移植组、SCs移植组、HCMNCs+SCs联合移植组.用HE染色、顺行示踪染色及电镜观察脊髓损伤处轴突再生情况,对各组实验动物脊髓损伤后肢体功能的恢复情况进行行为学评分(BBB评分)及脚印分析实验,综合评估脊髓功能恢复程度.结果 HCMNCs+SCs联合移植治疗能够明显促进神经再生,改善功能,减少空洞形成.BBB评分和脚印分析实验显示各组疗效比较,差异有统计学意义.组织学和电镜观察神经轴突再生情况与功能学检查的结果相吻合.结论以分泌各种神经营养因子为特点的SCs为平台,联合移植后诱导HCMNCs向神经元及神经胶质细胞分化,可促进脊髓损伤的恢复.
목적 탐토인제혈단개핵세포(haman cord blood mononuclear cells,HCMNCs)여대서설왕세포(Schwann cells,SCs)연합이식응용우대서척수손상적료효.방법 건강성년8주령Wistar자성대서40지,체중(200±30)g.이용Impactor Model Ⅱ형타격기제성T10척수손상모형.40지대서수궤분성4조,매조10지,즉DMEM실험대조조、HCMNCs이식조、SCs이식조、HCMNCs+SCs연합이식조.용HE염색、순행시종염색급전경관찰척수손상처축돌재생정황,대각조실험동물척수손상후지체공능적회복정황진행행위학평분(BBB평분)급각인분석실험,종합평고척수공능회복정도.결과 HCMNCs+SCs연합이식치료능구명현촉진신경재생,개선공능,감소공동형성.BBB평분화각인분석실험현시각조료효비교,차이유통계학의의.조직학화전경관찰신경축돌재생정황여공능학검사적결과 상문합.결론 이분비각충신경영양인자위특점적SCs위평태,연합이식후유도HCMNCs향신경원급신경효질세포분화,가촉진척수손상적회복.
Objective To explore the healing effects of co-transplanting human cord blood mononuclear cells(HCMNCs)and Schwann cell(SCs)for treatment of rat's spinal cord injury.Methods 40 Wistar rats,divided into 4 groups at random and evenly,were made into spinal cord injury model with Impactor Model Ⅱ at T10.The 4 groups were:DMEM control group,HCMNCs graft group,SCs graft group,HCMNCsSCs co-graft group.Anterograde tracing mark,HE staining and electronic microscope observation were used to observe axonal regeneration state in the iniured site.Rat's hindlimb functional recovery was evaluated by BBB locomotor functional Scale and footprint analysis.Results Co-graft HCMNCs and SCs could promote nerve regeneration,ameliorate functional recovery.and reduce cavity formation.There were statistic differences in healing effects between 4 groups in BBB locomotor functional scale and footprint analysis.Histology result and electronic microscope observation demonstrated axonal regeneration was consistent with functional recovery.Conclusion Serve SCs characterized by secreting various neumtrophic factors as platform,then co-graft HCMNCs which can be induced to differentiate into neuron and glial cells in order to promote recovery after spinal cord injury.