CAJ | 학술논문

目的观察腺相关病毒介导的骨形态发生蛋白-2(AAV-BMP-2)基因对兔退变腰椎间盘的治疗作用.方法将36只新西兰大白兔L2-3、L3-4、L4-5、L5-6椎间盘针刺制造退变模型后随机分为3组,其中12只注射AAV-BMP-2作为实验组,12只注射AAV作为实验对照组,12只注射生理盐水作为空白对照组.注射后的2、4、8周各组随机抽取4只兔行腰椎MRI扫描,扫描后将其处死,取L2-3、L3-4、L4-5、L5-6椎问盘髓核组织,用间苯三酚分光光度法检测髓核组织中的蛋白多糖含量.结果在MRI影像学Thompson分级评估中,实验组各时间点椎间盘MRI信号比较,差异无统计学意义.实验对照组和空白对照组各时间点MRI信号比较,差异有统计学意义.实验组和实验对照组、实验组和空白对照组各时间点MRI髓核信号比较,差异有统计学意义;实验对照组和空白对照组比较,差异无统计学意义.在蛋白多糖含量测定中,实验组蛋白多糖含量在各时间点均高于实验对照组和空白对照组,实验对照组和空白对照组蛋白多糖均随时间的变化逐渐减少,各时间点两组比较无明显区别.结论 AAV-BMP-2对兔退变的腰椎间盘有治疗作用.
목적 관찰선상관병독개도적골형태발생단백-2(AAV-BMP-2)기인대토퇴변요추간반적치료작용.방법 장36지신서란대백토L2-3、L3-4、L4-5、L5-6추간반침자제조퇴변모형후수궤분위3조,기중12지주사AAV-BMP-2작위실험조,12지주사AAV작위실험대조조,12지주사생리염수작위공백대조조.주사후적2、4、8주각조수궤추취4지토행요추MRI소묘,소묘후장기처사,취L2-3、L3-4、L4-5、L5-6추문반수핵조직,용간분삼분분광광도법검측수핵조직중적단백다당함량.결과 재MRI영상학Thompson분급평고중,실험조각시간점추간반MRI신호비교,차이무통계학의의.실험대조조화공백대조조각시간점MRI신호비교,차이유통계학의의.실험조화실험대조조、실험조화공백대조조각시간점MRI수핵신호비교,차이유통계학의의;실험대조조화공백대조조비교,차이무통계학의의.재단백다당함량측정중,실험조단백다당함량재각시간점균고우실험대조조화공백대조조,실험대조조화공백대조조단백다당균수시간적변화축점감소,각시간점량조비교무명현구별.결론 AAV-BMP-2대토퇴변적요추간반유치료작용.
Objective To evaluate the potential of bone morphogenetic protein-2(BMP-2)gene therapy application on rabbit interverbral disc degeneration,using the recombinant Adeno-associated virusvector expressing BMP-2.Methods L2-3,L3-4,L4-5,L5-6 lumbar discs of 36 skeletally mature New Zealand white rabbits were punctured to set up interverbral disc degeneration model.Subsequently,36 rabbits were randomly divided into three groups.Group 1(n=12)were injected with the Adeno-associated virus expressing BMP-2,Group 2(n=12)were injected with Adeno-associated virus only while Group 3(n=12)were injected with saline.Four rabbits were randomly selected from each group after iniection 2 weeks,4 weeks and 8 weeks respectively,and killed after lumbar MRI scanning.The L2-3,L3-4,L4-5,L5-6 lumbar discs of each rabbit was collected.Speetrophotometry were performed to determine proteoglycan synthesis.Results In Group 1,there were no significant difference in magnetic resonance imaging(MRI)at all time points.In constrast,in Group 2,Group 3,there were significant differences in MRI at all time points.The comparison among these three group showed that there were significant differences between Group 1 and Group 2,Group 1 and Group 3,while there were no significant differences between Group 2 and Group 3.The proteoglycan quantity in the nucleus pulposus comparison showed that there were significant differences between Group 1 and Group 2,Group 1 and Group 3,while there was no significant differences between Group 2 and Group 3.Conclusion Our study indicates that BMP-2 gene mediated by adeno-associated virus induced a restoration of intervertebral disc degeneration.