中华肝脏病杂志
中華肝髒病雜誌
중화간장병잡지
CHINESE JOURNAL OF HEPATOLOGY
2012年
12期
935-938
,共4页
胆汁淤积,肝内%白细胞介素10%肿瘤坏死因子α
膽汁淤積,肝內%白細胞介素10%腫瘤壞死因子α
담즙어적,간내%백세포개소10%종류배사인자α
Cholestasis,intrahepatic%Interleukin-10%Tumor necrosis factor-alpha
目的 探讨细胞因子信号传导负调控因子3 (SOCS3)、白细胞介素10 (IL-10)及肿瘤坏死因子α(TNFα)在妊娠期肝内胆汁淤积症(ICP)孕妇胎盘组织中的表达及其意义. 方法 选择2010年10月至2011年5月在新疆医科大学第一附属医院产科剖宫产分娩的ICP孕妇37例为病例组,选择同期健康孕妇35例为对照组.采用Envision免疫组织化学法测定IL-10、TNFα在孕妇胎盘组织中的定位与表达水平,Western blot测定胎盘组织中SOCS3的表达水平.组间比较采用t检验,等级资料采用秩和检验,变量间的相关性采用Spearman相关分析.结果 (1)IL-10、TNFα在两组孕妇胎盘组织中均有表达,在病例组中,IL-10的表达低于对照组(Z=-2.626,P＜0.01),而TNFα的表达则高于对照组(Z=-4.350,P＜0.01).(2)SOCS3在两组孕妇胎盘组织中均有表达,病例组的表达水平低于对照组(t=-2.214,P＜ 0.05).且其下降与IL-10呈正相关(r=0.494,P＜0.01),与TNFα呈负相关(r=-0.472,P＜0.01).结论 ICP患者母胎界面Th1型细胞因子TNFα表达增加,而Th2型细胞因子IL-10表达降低,存在Th1偏移.SOCS3可能通过影响细胞因子平衡而参与了ICP的发病.
目的 探討細胞因子信號傳導負調控因子3 (SOCS3)、白細胞介素10 (IL-10)及腫瘤壞死因子α(TNFα)在妊娠期肝內膽汁淤積癥(ICP)孕婦胎盤組織中的錶達及其意義. 方法 選擇2010年10月至2011年5月在新疆醫科大學第一附屬醫院產科剖宮產分娩的ICP孕婦37例為病例組,選擇同期健康孕婦35例為對照組.採用Envision免疫組織化學法測定IL-10、TNFα在孕婦胎盤組織中的定位與錶達水平,Western blot測定胎盤組織中SOCS3的錶達水平.組間比較採用t檢驗,等級資料採用秩和檢驗,變量間的相關性採用Spearman相關分析.結果 (1)IL-10、TNFα在兩組孕婦胎盤組織中均有錶達,在病例組中,IL-10的錶達低于對照組(Z=-2.626,P＜0.01),而TNFα的錶達則高于對照組(Z=-4.350,P＜0.01).(2)SOCS3在兩組孕婦胎盤組織中均有錶達,病例組的錶達水平低于對照組(t=-2.214,P＜ 0.05).且其下降與IL-10呈正相關(r=0.494,P＜0.01),與TNFα呈負相關(r=-0.472,P＜0.01).結論 ICP患者母胎界麵Th1型細胞因子TNFα錶達增加,而Th2型細胞因子IL-10錶達降低,存在Th1偏移.SOCS3可能通過影響細胞因子平衡而參與瞭ICP的髮病.
목적 탐토세포인자신호전도부조공인자3 (SOCS3)、백세포개소10 (IL-10)급종류배사인자α(TNFα)재임신기간내담즙어적증(ICP)잉부태반조직중적표체급기의의. 방법 선택2010년10월지2011년5월재신강의과대학제일부속의원산과부궁산분면적ICP잉부37례위병례조,선택동기건강잉부35례위대조조.채용Envision면역조직화학법측정IL-10、TNFα재잉부태반조직중적정위여표체수평,Western blot측정태반조직중SOCS3적표체수평.조간비교채용t검험,등급자료채용질화검험,변량간적상관성채용Spearman상관분석.결과 (1)IL-10、TNFα재량조잉부태반조직중균유표체,재병례조중,IL-10적표체저우대조조(Z=-2.626,P＜0.01),이TNFα적표체칙고우대조조(Z=-4.350,P＜0.01).(2)SOCS3재량조잉부태반조직중균유표체,병례조적표체수평저우대조조(t=-2.214,P＜ 0.05).차기하강여IL-10정정상관(r=0.494,P＜0.01),여TNFα정부상관(r=-0.472,P＜0.01).결론 ICP환자모태계면Th1형세포인자TNFα표체증가,이Th2형세포인자IL-10표체강저,존재Th1편이.SOCS3가능통과영향세포인자평형이삼여료ICP적발병.
Objective To detect the expression profiles of suppressor ofcytokine signaling 3 (SOCS3),interleukin (IL)-10,and tumor necrosis factor-alpha (TNF-α) in the placenta of women with intrahepatic cholestasis of pregnancy (ICP) and determine the cliincal significance of the differential expressions.Methods Placentas were collected from 37 ICP gravidas who delivered through cesarean section at the First Teaching Hospital of Xingjiang Medical University from October 2010 to May 2011 and from 35 healthy pregnant women (controls).SOCS3,TNF-α,and IL-10 protein levels were detected by immunoblotting and the Envision immunohistochernical method.Results TNF-α and IL-10 expression was detected in placentas of both groups,and was present mainly in the cytoplasm of trophoeytes.IL-10 expression was obviously lower in the ICP placentas than in the control placentas; meanwhile,TNF-α expression was obviously higher than in the control placentas (Z =-2.63,P ＜ 0.01).SOCS3 protein was significantly more abundant in the control placentas than in the ICP placentas.Furthermore,SOCS3 and IL-10 placental expressions were positively correlated (r =0.494,P ＜ 0.01),but there was a negative correlation between SOCS3 and TNF-α placental expressions (r =-0.472,P ＜ 0.01).Conclusion In ICP,an increase of the type 1 cytokine,TNF-α,is associated with decreases of the type 2 cytokine,IL-10,and of SOCS3,which may reduce the secretion of IL-10.Furthermore,SOCS3 may contribute to ICP pathogenesis by modulating the Th1/Th2 cytokine balance.
