CAJ | 학술논문

目的检测上皮间质转化(EMT)标志物E-cadherin和Vimentin在原发性肝癌及相邻正常组织中的蛋白表达水平,探讨E-adherin和Vimentin表达差异与肝癌转移相关性及预测临床预后意义. 方法收集30例原发性肝癌患者肝癌组织及相邻正常组织,Western blot荧光发光法定量检测E-cadherin,Vimentin蛋白在肝癌组织及相邻正常组织的表达,分析二者的蛋白表达相互关系及其与临床病理特征的相关性；同时采用免疫组织化学法检测Twist,Vimentin,E-cadherin在肝癌组织及相邻正常组织中的表达.数据统计采用配对t检验,Mann-Whitney U检验,spearman等级相关分析.结果 E-cadherin蛋白在原发性肝癌中肝癌组织较相邻正常组织表达显著降低,相对表达量为0.082±0.063对比0.226±0.215,差异具有统计学意义,t=-4.050,P＜0.01；其在门静脉癌栓患者肝癌组织中表达较无门静脉癌栓者降低(P=0.001),其在TNMⅢ期患者肝癌组织中表达较TNM Ⅰ/Ⅱ期表达降低(P=0.003)；Vimentin蛋白在原发性肝癌中癌组织表达较相邻正常组织表达增高(P=0.002),Vimentin蛋白表达与E-cadherin蛋白表达呈负相关(r=-0.509,P=0.004),Vimentin蛋白表达与门静脉癌栓(P＜0.01)、TNM分期(P＜0.01)、Milan标准(P=0.005)存在相关性；免疫组织化学结果显示E-cadherin在癌组织中几乎不表达,在相邻正常组织细胞膜上高表达,Vimentin和Twist均在肝癌组织细胞中高表达,而在相邻正常组织不表达.结论 EMT标志物E-cadherin和Vimentin蛋白在原发性肝癌中的表达与患者门静脉癌栓、TNM分期等临床特征密切相关,E-cadherin和Vimentin可作为预测肝癌转移复发及评估临床预后的有效指标.
목적 검측상피간질전화(EMT)표지물E-cadherin화Vimentin재원발성간암급상린정상조직중적단백표체수평,탐토E-adherin화Vimentin표체차이여간암전이상관성급예측림상예후의의. 방법 수집30례원발성간암환자간암조직급상린정상조직,Western blot형광발광법정량검측E-cadherin,Vimentin단백재간암조직급상린정상조직적표체,분석이자적단백표체상호관계급기여림상병리특정적상관성；동시채용면역조직화학법검측Twist,Vimentin,E-cadherin재간암조직급상린정상조직중적표체.수거통계채용배대t검험,Mann-Whitney U검험,spearman등급상관분석.결과 E-cadherin단백재원발성간암중간암조직교상린정상조직표체현저강저,상대표체량위0.082±0.063대비0.226±0.215,차이구유통계학의의,t=-4.050,P＜0.01；기재문정맥암전환자간암조직중표체교무문정맥암전자강저(P=0.001),기재TNMⅢ기환자간암조직중표체교TNM Ⅰ/Ⅱ기표체강저(P=0.003)；Vimentin단백재원발성간암중암조직표체교상린정상조직표체증고(P=0.002),Vimentin단백표체여E-cadherin단백표체정부상관(r=-0.509,P=0.004),Vimentin단백표체여문정맥암전(P＜0.01)、TNM분기(P＜0.01)、Milan표준(P=0.005)존재상관성；면역조직화학결과현시E-cadherin재암조직중궤호불표체,재상린정상조직세포막상고표체,Vimentin화Twist균재간암조직세포중고표체,이재상린정상조직불표체.결론 EMT표지물E-cadherin화Vimentin단백재원발성간암중적표체여환자문정맥암전、TNM분기등림상특정밀절상관,E-cadherin화Vimentin가작위예측간암전이복발급평고림상예후적유효지표.
Objective To determine the differential protein expressions of epithelial mesenchymal transition (EMT) markers E-cadherin and vimentin in hepatocellular carcinorma (HCC) and to investigate their correlation to the molecular mechanisms of metastasis to explore their potential utility as prognostic indicators of HCC.Methods Tumor tissues and patient-matched adjacent non-tumor tissues were collected from individuals diagnosed with HCC.E-cadherin and vimentin protein expressions in the tissue specimens were quantified by western blot with densitometry of fluorescence emission and comparatively analyzed to determine the associations with molecular and clinical features.The expressions of E-cadherin and vimentin,as well as the other EMT-related protein Twist,were also detected in the tissue specimens by immunohistochemistry.Statistical analyses were carried out by paired-samples t-test,Mann-Whitney test,and Spearman rank correlation analysis.Results E-cadherin expression was significantly lower in tumor tissues (0.082 ± 0.063 vs.adjacent non-tumor tissues:0.226 ± 0.215,t =-4.050,P ＜ 0.01),lower in patients with portal vein tumor thrombus (vs.non-thrombic HCC patients,P =0.001),and correlated with TNM stage (Ⅲ/Ⅳ ＞ Ⅰ/Ⅱ,P =0.003).Vimentin expression was significantly higher in tumor tissues (vs.adjacent non-tumor tissues,P =0.002),negatively correlated with E-cadherin expression (t =-0.509,P =0.004),and closely associated with some clinical parameters,such as portal vein tumor thrombus (P ＜ 0.01),TNM stage (P ＜ 0.01),and Milan criteria (P =0.005).Immunohistochemistry showed that E-cadherin expression was very weak in tumors but very strong in the cell membranes of non-tumor tissues,and that vimentin and Twist expressions were strong in tumors but undetectable in non-tumor tissue.Conclusion Expression levels of the EMT markers E-cadherin and vimentin in HCC are related to clinical parameters,including portal vein tumor thrombus and TNM stage,and may represent useful prognostic markers of metastasis.