中华肝脏病杂志
中華肝髒病雜誌
중화간장병잡지
CHINESE JOURNAL OF HEPATOLOGY
2013年
4期
299-303
,共5页
李艳丽%刘宁%赵丹彤%李卓敏%张海萍%刘燕敏%闫惠平%赵艳
李豔麗%劉寧%趙丹彤%李卓敏%張海萍%劉燕敏%閆惠平%趙豔
리염려%류저%조단동%리탁민%장해평%류연민%염혜평%조염
肝炎,自身免疫性%趋化因子类%生物化学指标%相关性研究
肝炎,自身免疫性%趨化因子類%生物化學指標%相關性研究
간염,자신면역성%추화인자류%생물화학지표%상관성연구
Hepatitis,autoimmune%Chemotactic factors%Biochemical indicators%Correlation analysis
目的 探讨自身免疫性肝炎(AIH)患者外周血中趋化因子(CCL2、CCL5、CXCL8、CXCL9和CXCL10)水平及其与肝脏生物化学指标的相关性. 方法 检测46例AIH患者血清中趋化因子(CCL2、CCL5、CXCL8、CXCL9和CXCL10)水平,选择12例健康体检者为对照组. 结果 AIH组CCL2、CXCL9和CXCL10水平分别为11.79 pg/ml、11.31 pg/ml和15.85 pg/ml,高于健康对照组的8.39 pg/ml、2.69 pg/ml、4.64 pg/ml,两组比较,Z值分别为-1.958、-4.527、-3.84,P值均< 0.05,差异均有统计学意义.AIH活动期组血清CCL2、CXCL8、CXCL9和CXCL10水平分别为29.69 pg/ml、7.2 pg/ml、16.02 pg/ml和90.01 pg/ml,明显高于激素治疗后缓解期组的11.16 pg/ml、5.38 pg/ml、5.47 pg/ml、13.24 pg/ml,两组比较,统计值分别为t=2.985、Z=-2.547、Z=-3.187、t=2.12,P值均<0.05,差异均有统计学意义.AIH患者血清CXCL8与IgG水平呈正相关关系(r2=0.291,P<0.05);CXCL9与ALT、AST水平均呈正相关关系(r2值分别为0.5324、0.3352,P值均<0.05);CXCL10与ALT、AST、GGT水平均呈正相关关系(r2值分别为0.9551、0.8960、0.8271,P值均<0.05). 结论 活动期AIH患者外周血中CCL2、CXCL8、CXCL9和CXCL10水平明显升高,CXCL9和CXCL10水平与肝脏炎症活动指标呈明显正相关关系,提示趋化因子水平可反映AIH肝脏炎症活动程度,在AIH病理损伤中发挥重要作用.
目的 探討自身免疫性肝炎(AIH)患者外週血中趨化因子(CCL2、CCL5、CXCL8、CXCL9和CXCL10)水平及其與肝髒生物化學指標的相關性. 方法 檢測46例AIH患者血清中趨化因子(CCL2、CCL5、CXCL8、CXCL9和CXCL10)水平,選擇12例健康體檢者為對照組. 結果 AIH組CCL2、CXCL9和CXCL10水平分彆為11.79 pg/ml、11.31 pg/ml和15.85 pg/ml,高于健康對照組的8.39 pg/ml、2.69 pg/ml、4.64 pg/ml,兩組比較,Z值分彆為-1.958、-4.527、-3.84,P值均< 0.05,差異均有統計學意義.AIH活動期組血清CCL2、CXCL8、CXCL9和CXCL10水平分彆為29.69 pg/ml、7.2 pg/ml、16.02 pg/ml和90.01 pg/ml,明顯高于激素治療後緩解期組的11.16 pg/ml、5.38 pg/ml、5.47 pg/ml、13.24 pg/ml,兩組比較,統計值分彆為t=2.985、Z=-2.547、Z=-3.187、t=2.12,P值均<0.05,差異均有統計學意義.AIH患者血清CXCL8與IgG水平呈正相關關繫(r2=0.291,P<0.05);CXCL9與ALT、AST水平均呈正相關關繫(r2值分彆為0.5324、0.3352,P值均<0.05);CXCL10與ALT、AST、GGT水平均呈正相關關繫(r2值分彆為0.9551、0.8960、0.8271,P值均<0.05). 結論 活動期AIH患者外週血中CCL2、CXCL8、CXCL9和CXCL10水平明顯升高,CXCL9和CXCL10水平與肝髒炎癥活動指標呈明顯正相關關繫,提示趨化因子水平可反映AIH肝髒炎癥活動程度,在AIH病理損傷中髮揮重要作用.
목적 탐토자신면역성간염(AIH)환자외주혈중추화인자(CCL2、CCL5、CXCL8、CXCL9화CXCL10)수평급기여간장생물화학지표적상관성. 방법 검측46례AIH환자혈청중추화인자(CCL2、CCL5、CXCL8、CXCL9화CXCL10)수평,선택12례건강체검자위대조조. 결과 AIH조CCL2、CXCL9화CXCL10수평분별위11.79 pg/ml、11.31 pg/ml화15.85 pg/ml,고우건강대조조적8.39 pg/ml、2.69 pg/ml、4.64 pg/ml,량조비교,Z치분별위-1.958、-4.527、-3.84,P치균< 0.05,차이균유통계학의의.AIH활동기조혈청CCL2、CXCL8、CXCL9화CXCL10수평분별위29.69 pg/ml、7.2 pg/ml、16.02 pg/ml화90.01 pg/ml,명현고우격소치료후완해기조적11.16 pg/ml、5.38 pg/ml、5.47 pg/ml、13.24 pg/ml,량조비교,통계치분별위t=2.985、Z=-2.547、Z=-3.187、t=2.12,P치균<0.05,차이균유통계학의의.AIH환자혈청CXCL8여IgG수평정정상관관계(r2=0.291,P<0.05);CXCL9여ALT、AST수평균정정상관관계(r2치분별위0.5324、0.3352,P치균<0.05);CXCL10여ALT、AST、GGT수평균정정상관관계(r2치분별위0.9551、0.8960、0.8271,P치균<0.05). 결론 활동기AIH환자외주혈중CCL2、CXCL8、CXCL9화CXCL10수평명현승고,CXCL9화CXCL10수평여간장염증활동지표정명현정상관관계,제시추화인자수평가반영AIH간장염증활동정도,재AIH병리손상중발휘중요작용.
Objective This study investigated circulation levels of chemokines (CCL2,CCL5,CXCL8,CXCL9,CXCL10) in autoimmune hepatitis(AIH) patients and evaluated the correlation between these chemokines and liver function indicators.Methods A total of 5 chemokines (CCL2,CCL5,CXCL8,CXCL9,CXCL10) were measured simultaneously by cytokine beads assay(CBA) in the sera of 46 patients with AIH and 12 cases of healthy control.Results In this study we found that serum levels of CCL2,CXCL9 and CXCL10 in AIH patients and healthy controls were 11.79:8.39 pg/ml,11.31:2.69 pg/ml,15.85:4.64 pg/mi,respectively,which implied these chemokines were significantly higher in AIH patients when compared to healthy control (Z=-1.958,P =0.05; Z=-4.527,P < 0.0001; Z=-3.84,P < 0.0001,respectively).And circulation levels of CCL2,CXCL8,CXCL9 and CXCL10 in pretreatment and remission stages of patients with AIH were 29.69:11.16 pg/ml,7.2:5.38 pg/ml,16.02:5.47 pg/ml,90.01:13.24 pg/ml,respectively,which showed these chemokines decreased during remission from pretreatment stage levels (t =2.985,P =0.005;Z=-2.547,P =0.0112; Z=-3.187,P =0.001; t =2.12,P =0.0015,respectively).Among AIH,CXCL8 was correlated positively with lgG(r2 =0.291,P =0.0039); CXCL9 was associated positively with ALT and AST(r2 =0.5324,P < 0.0001; r2 =0.3352,P < 0.0001); CXCL10 showed a positive correlation with ALT,AST and GGT(r2 =0.9551,P< 0.0001;r2 =0.8960,P< 0.0001;r2 =0.8271,P < 0.0001).Conclusion Serum levels of CCL2,CXCL8,CXCL9 and CXCL10 are significantly higher in patients with AIH,but decrease to levels in healthy controls after successful treatment,and circulation levels of CXCL9 and CXCL10 are associated positively with liver function indicators which can react inflammation activity of liver,all these may imply that chemokines can reflect the degree of liver inflammation and may be one of the main culprits in AIH pathological damage.