CAJ | 학술논문

目的观察妊娠肝内胆汁淤积症(ICP)胎盘CRHR-1的表达情况. 方法随机选择于37 ～ 40周择期剖宫产终止妊娠的ICP与正常妊娠各10例,采用Western blot及巢式实时荧光定量PCR测定胎盘CRHR-1表达强度.正态分布数据以均数土标准差(-x±s)表示,采用t检验.偏态分布数据采用中位数(M)及四分位距(IQR)表示为M(IQR),采用Wilcoxon秩和检验.检验水准a(双侧)=0.05. 结果 CRHR-1 cDNA目的扩增片段为172 bp.ICP组胎盘CRHR-1表达的免疫杂交蛋白条带荧光强度1.55±0.28弱于正常组1.60±0.37,但两组比较,差异无统计学意义(t=0.349,P=0.732);巢式实时荧光定量PCR扩增CRHR-1 mRNA相对含量0.139 (0.268)高于正常组的0.031 (0.245),两组比较,t=1.504,P=0.136,差异无统计学意义. 结论 ICP胎盘CRHR-1有低表达趋势,可能导致ICP胎盘小叶绒毛血管容积下降和CRH超短阳性反馈回路受损.
목적 관찰임신간내담즙어적증(ICP)태반CRHR-1적표체정황. 방법 수궤선택우37 ～ 40주택기부궁산종지임신적ICP여정상임신각10례,채용Western blot급소식실시형광정량PCR측정태반CRHR-1표체강도.정태분포수거이균수토표준차(-x±s)표시,채용t검험.편태분포수거채용중위수(M)급사분위거(IQR)표시위M(IQR),채용Wilcoxon질화검험.검험수준a(쌍측)=0.05. 결과 CRHR-1 cDNA목적확증편단위172 bp.ICP조태반CRHR-1표체적면역잡교단백조대형광강도1.55±0.28약우정상조1.60±0.37,단량조비교,차이무통계학의의(t=0.349,P=0.732);소식실시형광정량PCR확증CRHR-1 mRNA상대함량0.139 (0.268)고우정상조적0.031 (0.245),량조비교,t=1.504,P=0.136,차이무통계학의의. 결론 ICP태반CRHR-1유저표체추세,가능도치ICP태반소협융모혈관용적하강화CRH초단양성반궤회로수손.
Objective To explore the expression level of corticotropin-releasing hormone receptor type-1 (CRHR-1) in intrahepatic cholestatic placental (ICP) tissue.Methods Human placental samples were collected from 10 ICP patients and 10 healthy controls after parturition at 37-40 weeks of gestation.CRHR-1 protein and mRNA expression was assessed by western blotting and nested-real-time fluorescence quantitative PCR,respectively.Normally distributed data were summarized as mean ± standard deviation,and intergroup comparisons were made by two-tailed Student's t-test.Non-normally distributed data were presented as median with interquartile range,and intergroup comparisons were made by Wilcoxon test.For all statistical analyses,a two-tailed P-value of ＜ 0.05 was considered statistically significant.Results The CRHR-1 fluorescence intensity was lower in ICP tissues (1.55 ± 0.28) than in placental tissues from healthy controls (1.60 ± 0.37),but the difference did not reach statistical significance (t =0.349,P =0.732).The CRHR-1 mRNA content was slightly higher in the ICP tissues [0.139(0.268)] than in the placental tissues from healthy controls [0.031(0.245)],but the difference did not reach statistical significance (t =1.504,P =0.136).Conclusion CRHR-1 expression is decreased in ICP tissues,which may lead to a smaller volume of placental lobular villi vessels and restrict the CRH positive feedback loop,ultimately promoting acute hypoxic stress and possible harm to the fetus.