CAJ | 학술논문

目的探讨白细胞介素17 (IL-17)单核苷酸多态性(SNPs)及血浆水平与慢性HCV感染的关系.方法选择慢性丙型肝炎(CHC)患者228例,健康体检者81例.TaqMan探针法检测IL-17 rs8193036及rs2275913位点基因多态性,酶联免疫吸附法检测血浆IL-17水平,分析CHC患者IL-17等位基因、基因型及血浆水平与健康人群的差异.结果 CHC患者组与健康对照组IL-17 rs8193036位点C、T等位基因及rs2275913位点A、G等位基因分布比较,P值均＞0.05,差异均无统计学意义.CHC患者组与健康对照组的IL-17 rs8193036位点基因型CC (46.49％对比41.98％)、CT (45.61％对比44.44％)、TT(7.89％对比13.58％)分布比较,P值均＞0.05,差异均无统计学意义；rs2275913位点基因型AA(16.23％对比13.58％)、AG (48.25％对比50.62％)、GG(35.53％对比35.80％)分布比较,P值均＞0.05,差异均无统计学意义.HCV基因1型与2型感染CHC患者IL-17 rs8193036及rs2275913位点等位基因及基因型分布比较,P值均＞0.05,差异均无统计学意义.CHC患者组血浆IL-17水平为(97.67±39.68) pg/ml显著高于健康对照组的(71.60±19.78)pg/ml,两组比较t=2.414,P=0.033,差异有统计学意义.结论 IL-17高水平表达在HCV感染及慢性化中发挥重要作用,而IL-17 rs8193036及rs2275913位点SNPs可能与HCV易感性及持续感染无明确关系.
목적 탐토백세포개소17 (IL-17)단핵감산다태성(SNPs)급혈장수평여만성HCV감염적관계.방법 선택만성병형간염(CHC)환자228례,건강체검자81례.TaqMan탐침법검측IL-17 rs8193036급rs2275913위점기인다태성,매련면역흡부법검측혈장IL-17수평,분석CHC환자IL-17등위기인、기인형급혈장수평여건강인군적차이.결과 CHC환자조여건강대조조IL-17 rs8193036위점C、T등위기인급rs2275913위점A、G등위기인분포비교,P치균＞0.05,차이균무통계학의의.CHC환자조여건강대조조적IL-17 rs8193036위점기인형CC (46.49％대비41.98％)、CT (45.61％대비44.44％)、TT(7.89％대비13.58％)분포비교,P치균＞0.05,차이균무통계학의의；rs2275913위점기인형AA(16.23％대비13.58％)、AG (48.25％대비50.62％)、GG(35.53％대비35.80％)분포비교,P치균＞0.05,차이균무통계학의의.HCV기인1형여2형감염CHC환자IL-17 rs8193036급rs2275913위점등위기인급기인형분포비교,P치균＞0.05,차이균무통계학의의.CHC환자조혈장IL-17수평위(97.67±39.68) pg/ml현저고우건강대조조적(71.60±19.78)pg/ml,량조비교t=2.414,P=0.033,차이유통계학의의.결론 IL-17고수평표체재HCV감염급만성화중발휘중요작용,이IL-17 rs8193036급rs2275913위점SNPs가능여HCV역감성급지속감염무명학관계.
Objective To investigate the association of single nucleotide polymorphisms (SNPs) in the interleukin 17 (IL-17) gene and serum protein levels in patients with chronic hepatitis C virus (HCV) infection.Methods A total of 228 patients with chronic HCV infection and 81 healthy controls were enrolled in the study.The frequencies of IL-17 rs8193036 and rs2275913 polymorphisms were detected by the TaqMan SNP genotyping assay.Serum levels of IL-17 protein were detected by ELISA.Pairwise comparisons were made by the Chi-square test,and the significance of between-group diffrences was assessed by the Student's t-test with P ＜ 0.05.Results The patients with chronic HCV infection and the healthy controls showed similar frequencies of the rs8193036 C/T allele (x2 =1.428,P =0.232) and the rs2275913 A/G allele (x2 =0.106,P =0.744).In addition,the two groups showed similar distribution of the rs8193036 CC (chronic HCV infection:46.49％ vs.healthy controls:41.98％),CT (45.61％ vs.44.44％) and TT (7.89％ vs.13.58％)genotypes (x2 =2.346,P =0.309),and of the rs2275913 AA (16.23％ vs.13.58％),AG (48.25％ vs.50.62％) and GG (35.53％ vs.35.80％) genotypes (x2 =0.340,P =0.844).Subgroup analysis of chronic HCV infection patients stratified according to HCV genotypes 1 and 2 showed no differences in the distribution ofrs8193036 and rs2275913 alleles (x2 =1.127,P =0.288;x2 =1.088,P =0.297) and genotypes (x2 =2.825,P =0.246;x2 =0.970,P =0.616).However,the chronic HCV infection group did show significantly higher levels of serum IL-17 than the controls (97.67 + 39.68 vs.71.60 ± 19.78 pg/ml,t =2.414,P =0.033).Conclusion Chronic HCV infection is associated with increased serum IL-17; however,the IL-17 polymorphisms rs8193036 and rs2275913 were not associated with chronic HCV infection susceptibility in this study's Chinese cohort.