CAJ | 학술논문

目的了解多效生长因子(PTN)对大鼠肝细胞生长及白蛋白(Alb)分泌功能的影响. 方法根据培养液的不同,将体外培养的原代大鼠肝细胞分成3组,A组为空白对照组,B组加入3T3细胞培养上清液作为阳性对照组,C组加入人类重组PTN (hrPTN)作为实验组.比较3组肝细胞的生长情况,包括观察肝细胞的生长速度及分泌Alb的功能.组间差异采用单因素方差分析,各组之间的两两差异通过LSD法进行比较.结果 A、B、C组肝细胞生长率分别为2.800％±0.084％、4.300％±0.132％、3.800％±0.053％,B组肝细胞生长率明显高于A组和C组(F=333.735,P＜0.05).3组肝细胞上清液的Alb峰值集中在第2～6天,第2、第4及第6天,A组细胞上清液的Alb值分别为(0.550±0.010) g/L、(0.900±0.030) g/L和(0.300±0.040) g/L,B组分别为(0.900±0.030) g/L、(1.300±0.020) g/L和(1.400±0.030) g/L,C组分别为(0.900±0.010)g/L、(1.160±0.010) g/L和(0.700±0.050) g/L,方差分析结果均表明,B组促进肝细胞合成Alb的能力最强(F值分别为651.355、338.831和863.205,P值均＜0.05).结论 PTN能刺激肝细胞生长速度增快及分泌Alb功能增强,对大鼠肝细胞体外培养有促进作用.
목적 료해다효생장인자(PTN)대대서간세포생장급백단백(Alb)분비공능적영향. 방법 근거배양액적불동,장체외배양적원대대서간세포분성3조,A조위공백대조조,B조가입3T3세포배양상청액작위양성대조조,C조가입인류중조PTN (hrPTN)작위실험조.비교3조간세포적생장정황,포괄관찰간세포적생장속도급분비Alb적공능.조간차이채용단인소방차분석,각조지간적량량차이통과LSD법진행비교.결과 A、B、C조간세포생장솔분별위2.800％±0.084％、4.300％±0.132％、3.800％±0.053％,B조간세포생장솔명현고우A조화C조(F=333.735,P＜0.05).3조간세포상청액적Alb봉치집중재제2～6천,제2、제4급제6천,A조세포상청액적Alb치분별위(0.550±0.010) g/L、(0.900±0.030) g/L화(0.300±0.040) g/L,B조분별위(0.900±0.030) g/L、(1.300±0.020) g/L화(1.400±0.030) g/L,C조분별위(0.900±0.010)g/L、(1.160±0.010) g/L화(0.700±0.050) g/L,방차분석결과균표명,B조촉진간세포합성Alb적능력최강(F치분별위651.355、338.831화863.205,P치균＜0.05).결론 PTN능자격간세포생장속도증쾌급분비Alb공능증강,대대서간세포체외배양유촉진작용.
Objective To investigate the influence of pleiotrophin (PTN) on the growth of rat hepatocytes.Methods Primary rat hepatocytes were isolated from male Sprague-Dawley rats and divided into three groups:group A (negative control),cultivated in normal culture medium; group B (positive control),cultivated with culture medium supplemented with supernatant from the embryonic fibroblast 3T3 cell line; group C (experimetal),cultivated with culture medium supplemented with human recombinant (hr)PTN (100 ng/ml).The hepatocytes' growth rate and level of secreted albumin (ALB) were evaluated by microscopy and biochemical assay,respectively.Significance of between-group differences were assessed by one-way ANOVA,and pairwise comparisons were performed by the least significant difference test.Results The growth rates of hepatocytes in groups A,B and C were 2.800 ± 0.084％,4.300 ± 0.132％ and 3.800 ± 0.053％,respectively.The growth rate of group B was significantly higher than the other two groups (F =333.735,P ＜ 0.05).For all groups,the highest levels of secreted ALB were detected between the second and sixth day of culture,with g/L concentrations at day 2,4 and 6 of:group A,0.550 ± 0.010,0.900 ± 0.030 and 0.300 ± 0.040; group B,0.900 ± 0.030,1.300 ± 0.020 and 1.400 ± 0.030; group C,0.900 ± 0.010,1.160 ± 0.010 and 0.700 ± 0.050.The secreted ALB of group B was significantly higher than that of the other two groups (F=651.355,338.831 and 863.205,P ＜ 0.05).Conclusion PTN can benefit in vitro culturing of rat hepatocytes by stimulating growth and enhancing their ability to secrete albumin.