CAJ | 학술논문

目的应用凝集素亲和技术寻找肝癌高危人群进展为肝癌过程中的特征性血清糖蛋白凝集素亲和谱,并探讨其生物学意义.方法对广西肝癌高发区肝癌高危队列定期进行复查随访,将队列中陆续新发的肝癌、肝硬化、肝炎患者分别组成肝癌组、肝硬化组、肝炎组,并匹配正常对照组;应用凝集素芯片技术,比较肝癌组、肝硬化组、肝炎组及正常对照组的血清糖蛋白凝集素亲和谱,并用Western blot方法对有意义的差异性糖蛋白进行验证.采用独立样本t检验对组间亲和信号强度进行比较.结果在肝癌的发生过程中,血清糖蛋白表现出对橙黄网胞盘菌凝集素、尾穗苋凝集素(ACL)、伴刀豆凝集素(ConA)、小扁豆凝集素(LCA)、桑橙凝集素、槲寄生凝集素、红腰果E型凝集素(PHA-E)、红腰果L型凝集素(PHA-L)、豌豆凝集素(PSA)、蓖麻凝集素(RCA-I)、西泽接骨木凝集素(SNA)、马铃薯凝集素(STL)、槲寄生凝集素(VAL)和麦胚凝集素(WGA)14种凝集素的亲和信号增强(P＜ 0.05),提示在肝癌的发生过程中,患者血清里可能存在增多的岩藻糖、N-乙酰葡糖胺、N-乙酰半乳糖胺、甘露糖、平分型GlcNAc、末端β 1-4链接半乳糖等结构.将肝癌组的亲和信号增强与其他各组比较,除SNA在肝癌组与肝炎组的亲和信号强度差异无统计学意义(P＞0.05)外,其他凝集素的肝癌组与其他组比较,差异均有统计学意义(P值均＜0.05).结论广西肝癌高危人群从HBsAg阳性、肝炎、肝硬化,直至进展为肝癌的过程中,血清糖蛋白聚糖的变化具有特征性的差异,提示其与肝癌的发生和发展密切相关,为寻找肝癌早期诊断相关的糖标志物提供了重要参考.
목적 응용응집소친화기술심조간암고위인군진전위간암과정중적특정성혈청당단백응집소친화보,병탐토기생물학의의.방법 대엄서간암고발구간암고위대렬정기진행복사수방,장대렬중륙속신발적간암、간경화、간염환자분별조성간암조、간경화조、간염조,병필배정상대조조;응용응집소심편기술,비교간암조、간경화조、간염조급정상대조조적혈청당단백응집소친화보,병용Western blot방법대유의의적차이성당단백진행험증.채용독립양본t검험대조간친화신호강도진행비교.결과 재간암적발생과정중,혈청당단백표현출대등황망포반균응집소、미수현응집소(ACL)、반도두응집소(ConA)、소편두응집소(LCA)、상등응집소、곡기생응집소、홍요과E형응집소(PHA-E)、홍요과L형응집소(PHA-L)、완두응집소(PSA)、비마응집소(RCA-I)、서택접골목응집소(SNA)、마령서응집소(STL)、곡기생응집소(VAL)화맥배응집소(WGA)14충응집소적친화신호증강(P＜ 0.05),제시재간암적발생과정중,환자혈청리가능존재증다적암조당、N-을선포당알、N-을선반유당알、감로당、평분형GlcNAc、말단β 1-4련접반유당등결구.장간암조적친화신호증강여기타각조비교,제SNA재간암조여간염조적친화신호강도차이무통계학의의(P＞0.05)외,기타응집소적간암조여기타조비교,차이균유통계학의의(P치균＜0.05).결론 엄서간암고위인군종HBsAg양성、간염、간경화,직지진전위간암적과정중,혈청당단백취당적변화구유특정성적차이,제시기여간암적발생화발전밀절상관,위심조간암조기진단상관적당표지물제공료중요삼고.
Objective To identify specific serum glycoproteome profiles that correspond to the carcinogenic process of primary liver cancer (PLC) by analyzing a population with high-incidence of PLC using lectin affinity microarray.Methods Serum samples were collected from individuals classified as highrisk for PLC (including patients with liver cirrhosis and hepatitis B) and development of PLC was recorded.Healthy individuals served as normal controls.The serum samples were subjected to glycoproteome profling by using lectin microarrays and the results were confirmed by lectin blot.Between-group differences were statistically analyzed.Results PLC carcinogenesis was found to be correlated with enhanced affinity for AAL,ACL,ConA,LCA,MPL,NML,PHA-E,PHA-L,PSA,RCA-I,STL,VAL,WGA,and SNA (P ＜ 0.05).These data implied that changes in specific glycan structures,such as αFuc,GlcNAc,GalNAc,mannose,bisecting GlcNAc and terminal β1-4 Gal,may be involved in PLC carcinogenesis.The PLC group showed significantly different results for all detected lectins,except SNA (P ＜ 0.05).However,among the PLC group,the SNA affinity was not significantly different for the hepatitis B group (P =0.443,P ＞ 0.05).Conclusion Glycans may be associated with the carcinogenic process of PLC and may be developed as diagnostic and prognostic biomarkers of PLC in the future.