中华肝脏病杂志
中華肝髒病雜誌
중화간장병잡지
CHINESE JOURNAL OF HEPATOLOGY
2014年
5期
374-379
,共6页
牛同红%姜曼%刘昊刚%姜相君%林中华%张梅%王健%耿宁%辛永宁
牛同紅%薑曼%劉昊剛%薑相君%林中華%張梅%王健%耿寧%辛永寧
우동홍%강만%류호강%강상군%림중화%장매%왕건%경저%신영저
脂肪肝,非酒精性%单核苷酸多态性%载脂蛋白C3
脂肪肝,非酒精性%單覈苷痠多態性%載脂蛋白C3
지방간,비주정성%단핵감산다태성%재지단백C3
Fatty liver,nonalcoholic%Single neucleotide polymorphism%Apolipoprotein C3
目的 探讨载脂蛋白(APO) C3基因启动子区两位点多态性T-455C、C-482T与非酒精性脂肪性肝病(NAFLD)遗传易感性的相关性.方法 对287例NAFLD患者和310名正常对照采用多聚酶链反应(PCR)及基因型检测方法对两变异位点进行基因型检测.随机选取180人采用酶联免疫吸附试验(ELISA)测定APOC3的含量.并比较各组基因型频率、等位基因频率及临床资料.根据数据资料的不同,分别行Pearson x2检验、logistic回归分析、t检验、x 2检验或行线性回归分析.结果 -455、-482位点基因型及等位基因在NAFLD组与对照组中的分布频率差异无统计学意义(P＞0.05).相对于野生型-455TT或-482CC,含突变位点基因型未增加NAFLD的发病风险,经多变量logistic回归分析校正混杂因素后,含等位基因-455C、-482T的基因型携带者发生NAFLD的比值比(OR及95％CI)分别为1.25 (0.79～ 1.96)及1.20 (0.76 ～ 1.89),P＞0.05.临床资料及各生物化学指标在野生型组及含突变位点组中均未见明显差异(P＞ 0.05).结论 在汉族人群中,APOC3基因启动子区两位点多态性与NAFLD的发生无明显相关性.
目的 探討載脂蛋白(APO) C3基因啟動子區兩位點多態性T-455C、C-482T與非酒精性脂肪性肝病(NAFLD)遺傳易感性的相關性.方法 對287例NAFLD患者和310名正常對照採用多聚酶鏈反應(PCR)及基因型檢測方法對兩變異位點進行基因型檢測.隨機選取180人採用酶聯免疫吸附試驗(ELISA)測定APOC3的含量.併比較各組基因型頻率、等位基因頻率及臨床資料.根據數據資料的不同,分彆行Pearson x2檢驗、logistic迴歸分析、t檢驗、x 2檢驗或行線性迴歸分析.結果 -455、-482位點基因型及等位基因在NAFLD組與對照組中的分佈頻率差異無統計學意義(P＞0.05).相對于野生型-455TT或-482CC,含突變位點基因型未增加NAFLD的髮病風險,經多變量logistic迴歸分析校正混雜因素後,含等位基因-455C、-482T的基因型攜帶者髮生NAFLD的比值比(OR及95％CI)分彆為1.25 (0.79～ 1.96)及1.20 (0.76 ～ 1.89),P＞0.05.臨床資料及各生物化學指標在野生型組及含突變位點組中均未見明顯差異(P＞ 0.05).結論 在漢族人群中,APOC3基因啟動子區兩位點多態性與NAFLD的髮生無明顯相關性.
목적 탐토재지단백(APO) C3기인계동자구량위점다태성T-455C、C-482T여비주정성지방성간병(NAFLD)유전역감성적상관성.방법 대287례NAFLD환자화310명정상대조채용다취매련반응(PCR)급기인형검측방법대량변이위점진행기인형검측.수궤선취180인채용매련면역흡부시험(ELISA)측정APOC3적함량.병비교각조기인형빈솔、등위기인빈솔급림상자료.근거수거자료적불동,분별행Pearson x2검험、logistic회귀분석、t검험、x 2검험혹행선성회귀분석.결과 -455、-482위점기인형급등위기인재NAFLD조여대조조중적분포빈솔차이무통계학의의(P＞0.05).상대우야생형-455TT혹-482CC,함돌변위점기인형미증가NAFLD적발병풍험,경다변량logistic회귀분석교정혼잡인소후,함등위기인-455C、-482T적기인형휴대자발생NAFLD적비치비(OR급95％CI)분별위1.25 (0.79～ 1.96)급1.20 (0.76 ～ 1.89),P＞0.05.림상자료급각생물화학지표재야생형조급함돌변위점조중균미견명현차이(P＞ 0.05).결론 재한족인군중,APOC3기인계동자구량위점다태성여NAFLD적발생무명현상관성.
Objective To investigate the association between two polymorphisms of the APOC3 gene (T-455C and C-482T) and hereditary risk of non-alcoholic fatty liver disease (NAFLD).Methods A total of 287 patients with NAFLD and 310 control subjects were genotyped by PCR and direct sequencing.Serum lipid profiles were also detected by standard biochemical methods.One-hundred-and-eighty of the study participants were used to measure the APOC3 content by enzyme-linked immunosorbent assay.Inter-group differences and associations were assessed statistically using Chi square and t tests and logistic and linear regression analyses.Results The frequencies of neither the genotypes or alleles were significantly different between the NAFLD cases and the controls.Compared with the most common genotypes-455TT or-482CC,none of the variants showed a significant increase in risk of NAFLD or for the clinical and biochemical parameters.The adjusted odds ratios (with 95％ confidence intervals) of NAFLD were 1.25 (0.79-1.96) and 1.20 (0.76-1.89) for carriers of the APOC3-455C and-482 T variants respectively (P ＞ 0.05).Conclusion The T-455C and C-482T polymorphisms of the APOC3 gene are not associated with risk of NAFLD,pathogenic changes in lipid profiles,or insulin resistance in Han Chinese.
