CAJ | 학술논문

目的通过对单中心肝移植术后乙型肝炎复发的监测和HBV-S基因变异的检测,旨在初步了解肝移植术后乙型肝炎复发患者S基因变异与乙型肝炎复发之间的关系. 方法随访单中心299例HBV相关肝病肝移植患者,荧光定量聚合酶链反应扩增乙型肝炎复发患者血清HBV DNA,采用Sanger酶学法检测HBV-S基因.用cox回归分析法分析乙型肝炎重发因素. 结果肝移植患者术后共有12例发生乙型肝炎复发,其中2例发生了HBV-S基因变异,肝移植术后乙型肝炎复发患者S基因变异率为16.67％ (2/12),T126I合并G145A变异和M133L变异患者各1例.Cox多因素分析提示:HBV-YMDD变异(P=0.0l)、HBV-S基因变异(P=0.03)、治疗依从性下降(P=0.03)均是乙型肝炎复发的风险因素. 结论 HBV-S基因变异是可能导致HBV相关肝病肝移植术后乙型肝炎复发的原因之一,值得深入研究.
목적 통과대단중심간이식술후을형간염복발적감측화HBV-S기인변이적검측,지재초보료해간이식술후을형간염복발환자S기인변이여을형간염복발지간적관계. 방법 수방단중심299례HBV상관간병간이식환자,형광정량취합매련반응확증을형간염복발환자혈청HBV DNA,채용Sanger매학법검측HBV-S기인.용cox회귀분석법분석을형간염중발인소. 결과 간이식환자술후공유12례발생을형간염복발,기중2례발생료HBV-S기인변이,간이식술후을형간염복발환자S기인변이솔위16.67％ (2/12),T126I합병G145A변이화M133L변이환자각1례.Cox다인소분석제시:HBV-YMDD변이(P=0.0l)、HBV-S기인변이(P=0.03)、치료의종성하강(P=0.03)균시을형간염복발적풍험인소. 결론 HBV-S기인변이시가능도치HBV상관간병간이식술후을형간염복발적원인지일,치득심입연구.
Objective To examine the incidence of hepatitis B virus (HBV) S gene mutation in recipients with recurrent HBV infection after liver transplantation (LT) and to evaluate the clinical significance of these mutants.Methods Two-hundred-and-ninety-nine patients who received LT for HBV-related liver diseases in single centre were enrolled in the study and followed up.Serum HBV DNA was amplified by fluorescence quantitative polymerase chain reaction,and HBV-S gene mutation was detected by Sanger's enzymatic method.Results Twelve of the 299 patients developed recurrent HBV after LT,and 2 of these 12 carried a mutant of the HBV-S gene (incidence rate of 16.67％).One of the patients had T126I and G145A mutations,and the other had a M 133L mutation.Cox regression modelling identified the risk factors of HBV recurrence after LT as HBV-YMDD mutants (P =0.01),HBV-S mutants (P =0.03) and compliance decrease (P =0.03).Conclusion HBV-S mutants may contribute to recurrence of HBV after LT,and the mechanism should be addressed in future studies.