CAJ | 학술논문

目的研究叉头样转录因子3 (Foxp3)的表达及启动子区甲基化水平在肝细胞性肝癌(HCC)与正常肝组织中的差异及其在HCC发病中的作用. 方法使用荧光定量PCR检测39例HCC及13例正常肝组织中Foxp3 mRNA水平,通过焦磷酸测序的方法检测其启动子区甲基化水平;组间比较采用秩和检验,相关性分析采用Spearman秩相关,P＜0.05为差异有统计学意义.结果 Foxp3 mRNA在肝癌组织中相对表达量为8.54±18.30,高于正常肝组织的4.88±14.72,两组比较,Z=-2.770,P=0.0056,差异有统计学意义.Foxp3启动子区A位点的甲基化水平在正常肝组织为90.60±3.50,高于肝癌组织的80.96±20.86,两组比较,Z=2.118,P=0.0339,差异有统计学意义;而正常肝组织和肝癌组织Foxp3启动子区其余B、C、D、E 4个位点和整体甲基化水平比较,P值均＞ 0.05,差异均无统计学意义.Foxp3 mRNA水平与A位点的甲基化水平相关性分析,r=-0.344,P=0.046,呈负相关;而其余4个位点与Foxp3 mRNA水平均无相关性(P值均＞ 0.05). 结论 Foxp3高表达与Foxp3启动子区A位点低甲基化可能参与肝癌的发生和发展.
목적 연구차두양전록인자3 (Foxp3)적표체급계동자구갑기화수평재간세포성간암(HCC)여정상간조직중적차이급기재HCC발병중적작용. 방법 사용형광정량PCR검측39례HCC급13례정상간조직중Foxp3 mRNA수평,통과초린산측서적방법검측기계동자구갑기화수평;조간비교채용질화검험,상관성분석채용Spearman질상관,P＜0.05위차이유통계학의의.결과 Foxp3 mRNA재간암조직중상대표체량위8.54±18.30,고우정상간조직적4.88±14.72,량조비교,Z=-2.770,P=0.0056,차이유통계학의의.Foxp3계동자구A위점적갑기화수평재정상간조직위90.60±3.50,고우간암조직적80.96±20.86,량조비교,Z=2.118,P=0.0339,차이유통계학의의;이정상간조직화간암조직Foxp3계동자구기여B、C、D、E 4개위점화정체갑기화수평비교,P치균＞ 0.05,차이균무통계학의의.Foxp3 mRNA수평여A위점적갑기화수평상관성분석,r=-0.344,P=0.046,정부상관;이기여4개위점여Foxp3 mRNA수평균무상관성(P치균＞ 0.05). 결론 Foxp3고표체여Foxp3계동자구A위점저갑기화가능삼여간암적발생화발전.
Objective To gain insights into the role of forkhead box protein 3 (Foxp3) in the pathogenesis of hepatocellular carcinoma (HCC) by performing a comparative analysis of Foxp3 mRNA expression and promoter methylation status in HCC and normal liver tissues.Methods Thirty-nine HCC and 13 normal liver tissue specimens were evaluated by real-time quantitative PCR and pyrosequencing to measure the expression of Foxp3 mRNA and determine the methylation status of its promoter,respectively.Statistical analyses of the data were conducted by rank-sum test and Spearman's rank correlation coefficient test.Results The HCC specimens showed significantly higher mRNA expression of Foxp3 (vs.normal liver tissues,Z =-2.770,P =0.0056).Moreover,the HCC specimens showed significant hypomethylation of the Foxp3 promoter site A (vs.normal liver tissues,Z =2.118,P =0.0339),and the Foxp3 mRNA level was negatively correlated with the methylation of site A (rs =-0.344,P =0.046).None of the other four sites in the Foxp3 promoter showed a significant difference in methylation,and the overall methylation was not significantly different between the HCC and normal liver tissues.Conclusion Overexpression and low methylation of Foxp3 may be involved in the oncogenic and progression processes of HCC.