中华肝脏病杂志
中華肝髒病雜誌
중화간장병잡지
CHINESE JOURNAL OF HEPATOLOGY
2014年
9期
676-679
,共4页
谭盛葵%王维伟%刘顺%韦茜茜%韦佳如%王志刚%闫萌%仇小强
譚盛葵%王維偉%劉順%韋茜茜%韋佳如%王誌剛%閆萌%仇小彊
담성규%왕유위%류순%위천천%위가여%왕지강%염맹%구소강
癌,肝细胞%多态性,单核苷酸%病例对照研究%交互作用
癌,肝細胞%多態性,單覈苷痠%病例對照研究%交互作用
암,간세포%다태성,단핵감산%병례대조연구%교호작용
Carcinoma,hepatocellular%Polymorphism,single nucleotide%Case-control study%Interactions
目的 研究hMSH2基因多态性和环境因素的交互作用与肝细胞癌易感性的关系.方法 采用以医院为基础的病例对照研究方法,以肝细胞癌患者(病例组)和非肿瘤患者(对照组)为研究对象,用荧光定量PCR法检测hMSH2基因,用非条件logistic回归法分析hMSH2基因型在两组中分布频率的差异,以及基因多态性和环境因素的交互作用.对计量资料和计数资料分别进行t检验和x2检验,因素间的交互作用采用非条件logistic回归模型进行分析.结果 病例组中hMSH2基因rs2303428位点CC、CT和TT各基因型频率分别为14.13％、47.02％和38.85％,对照组中3种基因型频率分别为8.21％、49.47％和42.32％,差异有统计学意义(x 2=8.289,P=0.016).T等位基因携带者患肝细胞癌的危险性是C等位基因携带者的1.228倍.交互作用分析结果表明hMSH2基因rs2303428多态性与肿瘤家族史、HBsAg阳性在肝细胞癌发生中存在交互作用,比值比分别为1.865和5.634.而hMSH2基因rs4952887、rs2059520与肝细胞癌的发生、发展无关,且与环境因素无明显交互作用.结论 hMSH2基因rs2303428可能是广西南部地区人群肝细胞癌的危险因素之一,其多态性与肿瘤家族史、HBsAg阳性在肝细胞癌发生中存在交互作用,能增加罹患肝细胞癌的风险.
目的 研究hMSH2基因多態性和環境因素的交互作用與肝細胞癌易感性的關繫.方法 採用以醫院為基礎的病例對照研究方法,以肝細胞癌患者(病例組)和非腫瘤患者(對照組)為研究對象,用熒光定量PCR法檢測hMSH2基因,用非條件logistic迴歸法分析hMSH2基因型在兩組中分佈頻率的差異,以及基因多態性和環境因素的交互作用.對計量資料和計數資料分彆進行t檢驗和x2檢驗,因素間的交互作用採用非條件logistic迴歸模型進行分析.結果 病例組中hMSH2基因rs2303428位點CC、CT和TT各基因型頻率分彆為14.13％、47.02％和38.85％,對照組中3種基因型頻率分彆為8.21％、49.47％和42.32％,差異有統計學意義(x 2=8.289,P=0.016).T等位基因攜帶者患肝細胞癌的危險性是C等位基因攜帶者的1.228倍.交互作用分析結果錶明hMSH2基因rs2303428多態性與腫瘤傢族史、HBsAg暘性在肝細胞癌髮生中存在交互作用,比值比分彆為1.865和5.634.而hMSH2基因rs4952887、rs2059520與肝細胞癌的髮生、髮展無關,且與環境因素無明顯交互作用.結論 hMSH2基因rs2303428可能是廣西南部地區人群肝細胞癌的危險因素之一,其多態性與腫瘤傢族史、HBsAg暘性在肝細胞癌髮生中存在交互作用,能增加罹患肝細胞癌的風險.
목적 연구hMSH2기인다태성화배경인소적교호작용여간세포암역감성적관계.방법 채용이의원위기출적병례대조연구방법,이간세포암환자(병례조)화비종류환자(대조조)위연구대상,용형광정량PCR법검측hMSH2기인,용비조건logistic회귀법분석hMSH2기인형재량조중분포빈솔적차이,이급기인다태성화배경인소적교호작용.대계량자료화계수자료분별진행t검험화x2검험,인소간적교호작용채용비조건logistic회귀모형진행분석.결과 병례조중hMSH2기인rs2303428위점CC、CT화TT각기인형빈솔분별위14.13％、47.02％화38.85％,대조조중3충기인형빈솔분별위8.21％、49.47％화42.32％,차이유통계학의의(x 2=8.289,P=0.016).T등위기인휴대자환간세포암적위험성시C등위기인휴대자적1.228배.교호작용분석결과표명hMSH2기인rs2303428다태성여종류가족사、HBsAg양성재간세포암발생중존재교호작용,비치비분별위1.865화5.634.이hMSH2기인rs4952887、rs2059520여간세포암적발생、발전무관,차여배경인소무명현교호작용.결론 hMSH2기인rs2303428가능시엄서남부지구인군간세포암적위험인소지일,기다태성여종류가족사、HBsAg양성재간세포암발생중존재교호작용,능증가리환간세포암적풍험.
Objective To use a hospital-based case-control study design to investigate the relationship between hepatocellular carcinoma (HCC) and the interaction of polymorphisms in the human mismatch repair gene,hMSH2,with environmental factors.Methods Cases of new-onset,histopathology-diagnosed,and untreated (no chemotherapy or radiation therapy) HCC were enrolled between September 2009 and September 2012.A non-HCC healthy control group was also enrolled and was composed of individuals living in the same region as the cases for ＞ 10 years and age-/sex-matched with similar socioeconomic characteristics.All enrollees underwent hMSH2 genotyping by real-time PCR.T-test,chi-square test and unconditional logistic regression analysis was used to analyze the difference in allele frequencies among the case and control groups and the relationship between hMSH2 polymorphisms and environmental factors.Results Frequencies of hMSH2 rs2303428 CC,CT and TT genotypes in the HCC group were significantly different than in the control group (14.13％ vs.8.21％,47.02％ vs.49.47％,and 38.85％ vs.42.32％;x 2=8.289,P =0.016).Individuals carrying the hMSH2 rs2303428 T allelic gene had a significantly increased risk compared to those with the hMSH2 rs2303428 C allelic gene (adjusted OR=1.228).Interactions were found between the hMSH2 genotype and hepatitis B surface antigen (HBsAg)-positive hepatitis infection (adjusted OR=1.865) and history of cancer (adjusted OR=5.634).There was no relation between hMSH2 gene rs4952887 and rs2059520 and liver cancer development or interaction with environmental factors.Conclusion The hMSH2 rs2303428 genotype is positively related to risk of HCC in Chinese,with HBsAg-positive hepatitis infection starus and history of cancer increasing the risk.
