中华肝脏病杂志
中華肝髒病雜誌
중화간장병잡지
CHINESE JOURNAL OF HEPATOLOGY
2014年
11期
831-836
,共6页
癌,肝细胞%多态性,单核苷酸%基因,着色性干皮病基因D%基因,谷胱甘肽-S-转移酶M1
癌,肝細胞%多態性,單覈苷痠%基因,著色性榦皮病基因D%基因,穀胱甘肽-S-轉移酶M1
암,간세포%다태성,단핵감산%기인,착색성간피병기인D%기인,곡광감태-S-전이매M1
Carcinoma,hepatocellular%Polymorphism,single nucleotide%Gene,xerodera pigmentosum group D%Gene,glutathione-S transferees M1
目的 探讨青海地区藏族人群着色性干皮病基因D(XPD)、谷胱甘肽-S-转移酶(GST)M1基因多态性与原发性肝癌(PHC)易感性的关系. 方法 采用病例对照研究,选择青海地区藏族PHC患者及同期藏族健康体检者各102例,用PCR、变性高效液相色谱技术进行基因分型检测,以非条件logistic逐步回归模型进行PHC危险因素的多变量分析,比较不同基因型与PHC患病风险的关系.计数资料采用x2检验,以比值比(OR)及其95％可信区间(CI)表示相对危险度. 结果 吸烟、肉食、饮酒、HBV感染、直系亲属HBV感染、直系亲属肝癌等均进入logistic回归模型(α=0.05).XPD751C突变基因型在病例组和对照组的分布频率分别为21.6％和10.8％,组间差异有统计学意义(x2=4.374,P=0.036),罹患PHC的风险OR为2.275(95％CI为1.04～4.98).GSTM1空白基因型在病例组和对照组的分布频率分别为60.8％和44.1％,组间差异有统计学意义(x2=5.680,P=0.017);携带GSTM1空白基因型者患病风险是携带GSTM1非空白基因型者的1.963倍(95％ CI为1.124 ～ 3.428).将XPD751C基因突变和GSTM1空白联合基因型作为暴露因素,罹患肝癌的风险OR为3.030 (95％ CI为1.165 ～ 7.881);XPD751C突变基因型与HBV感染、饮酒、家族直系亲属肝癌等因素有交互作用.结论 吸烟、饮酒、肉食、HBV感染、直系亲属HBV感染、直系亲属患肝癌等是青海藏族人群罹患PHC的主要环境危险因素;XPD751C突变等位基因型、GSTM1空白基因型是青海藏族人群PHC的易感因素;携带XPD751C突变和GSTM1空白联合基因型的个体,比单个基因型患病风险显著增加;青海藏族人群携带XPD751C突变等位基因型的个体可分别与HBV感染、饮酒、家族直系亲属肝癌3种环境危险因素共同诱导PHC的发生.
目的 探討青海地區藏族人群著色性榦皮病基因D(XPD)、穀胱甘肽-S-轉移酶(GST)M1基因多態性與原髮性肝癌(PHC)易感性的關繫. 方法 採用病例對照研究,選擇青海地區藏族PHC患者及同期藏族健康體檢者各102例,用PCR、變性高效液相色譜技術進行基因分型檢測,以非條件logistic逐步迴歸模型進行PHC危險因素的多變量分析,比較不同基因型與PHC患病風險的關繫.計數資料採用x2檢驗,以比值比(OR)及其95％可信區間(CI)錶示相對危險度. 結果 吸煙、肉食、飲酒、HBV感染、直繫親屬HBV感染、直繫親屬肝癌等均進入logistic迴歸模型(α=0.05).XPD751C突變基因型在病例組和對照組的分佈頻率分彆為21.6％和10.8％,組間差異有統計學意義(x2=4.374,P=0.036),罹患PHC的風險OR為2.275(95％CI為1.04～4.98).GSTM1空白基因型在病例組和對照組的分佈頻率分彆為60.8％和44.1％,組間差異有統計學意義(x2=5.680,P=0.017);攜帶GSTM1空白基因型者患病風險是攜帶GSTM1非空白基因型者的1.963倍(95％ CI為1.124 ～ 3.428).將XPD751C基因突變和GSTM1空白聯閤基因型作為暴露因素,罹患肝癌的風險OR為3.030 (95％ CI為1.165 ～ 7.881);XPD751C突變基因型與HBV感染、飲酒、傢族直繫親屬肝癌等因素有交互作用.結論 吸煙、飲酒、肉食、HBV感染、直繫親屬HBV感染、直繫親屬患肝癌等是青海藏族人群罹患PHC的主要環境危險因素;XPD751C突變等位基因型、GSTM1空白基因型是青海藏族人群PHC的易感因素;攜帶XPD751C突變和GSTM1空白聯閤基因型的箇體,比單箇基因型患病風險顯著增加;青海藏族人群攜帶XPD751C突變等位基因型的箇體可分彆與HBV感染、飲酒、傢族直繫親屬肝癌3種環境危險因素共同誘導PHC的髮生.
목적 탐토청해지구장족인군착색성간피병기인D(XPD)、곡광감태-S-전이매(GST)M1기인다태성여원발성간암(PHC)역감성적관계. 방법 채용병례대조연구,선택청해지구장족PHC환자급동기장족건강체검자각102례,용PCR、변성고효액상색보기술진행기인분형검측,이비조건logistic축보회귀모형진행PHC위험인소적다변량분석,비교불동기인형여PHC환병풍험적관계.계수자료채용x2검험,이비치비(OR)급기95％가신구간(CI)표시상대위험도. 결과 흡연、육식、음주、HBV감염、직계친속HBV감염、직계친속간암등균진입logistic회귀모형(α=0.05).XPD751C돌변기인형재병례조화대조조적분포빈솔분별위21.6％화10.8％,조간차이유통계학의의(x2=4.374,P=0.036),리환PHC적풍험OR위2.275(95％CI위1.04～4.98).GSTM1공백기인형재병례조화대조조적분포빈솔분별위60.8％화44.1％,조간차이유통계학의의(x2=5.680,P=0.017);휴대GSTM1공백기인형자환병풍험시휴대GSTM1비공백기인형자적1.963배(95％ CI위1.124 ～ 3.428).장XPD751C기인돌변화GSTM1공백연합기인형작위폭로인소,리환간암적풍험OR위3.030 (95％ CI위1.165 ～ 7.881);XPD751C돌변기인형여HBV감염、음주、가족직계친속간암등인소유교호작용.결론 흡연、음주、육식、HBV감염、직계친속HBV감염、직계친속환간암등시청해장족인군리환PHC적주요배경위험인소;XPD751C돌변등위기인형、GSTM1공백기인형시청해장족인군PHC적역감인소;휴대XPD751C돌변화GSTM1공백연합기인형적개체,비단개기인형환병풍험현저증가;청해장족인군휴대XPD751C돌변등위기인형적개체가분별여HBV감염、음주、가족직계친속간암3충배경위험인소공동유도PHC적발생.
Objective To explore the relationship of polymorphisms in the xeroderma pigmentosum group D (XPD) gene and the glutathione-S transferees M1 (GSTM1) gene with susceptibility to primary hepatic carcinoma (PHC) in Tibetans from the Qinghai region.Methods This case-control study compared equal groups (n =102 each) of patients with PHC and healthy individuals recruited from Qinghai,Tibet.PCR and denaturing high-performance liquid chromatography (DHPLC) was used to detect each participant's genotypes for the XPD and GSTM1 genes.Non-conditional logistic regression modeling was used in multivariate analysis to evaluate the predictive value for PHC,to compare the risk of different genotypes for PHC,and to assess the risk of gene polymorphisms and environmental factors for PHC.Results Six factors,including smoking,carnivorous diet,alcohol consumption,hepatitis B virus (HBV) infection,immediate family members with HBV infection and immediate family members with history of PHC,were included in the logistic regression model (α =0.05).The XPD751C mutation genotype distribution frequencies were significantly higher in the cases than in the controls (21.6％ vs.10.8％,P =0.036).The risk of PHC increased 2.275 times (95％ CI,1.04-4.98).The frequencies of the GSTM1 genotype were remarkably higher in the cases than in the controls (60.4％ vs.39.6％,P =0.017),suggesting this as an exposure factor.Individuals with the GSTM1 genotype had 1.963 times higher risk of PHC than individuals without the GSTM1 genotype (95％ CI,1.124-3.428).With both the XPD751C mutation and the GSTM1 genotype as exposure factors,the risk incidence increased to 3.030 times (95％ CI,1.165-7.881),indicating that the combined genotypes have a synergistic effect.Application of unconditioned logistic stepwise regression analysis of the genotypes and environmental risk factors showed an interaction between the XPD751C mutation and HBV infection,alcohol consumption and immediate family members with history of PHC.In addition,an interaction between the GSTM1 genotype and HBV infection was found.Conclusion Alcohol consumption,HBV infection and the presence of immediate family members with HBV infection are the main environmental risk factors of PHC in Qinghai Tibetans.Qinghai Tibetans who carry the XPD751C gene mutation and the GSTM 1 genotype are at increased risk of PHC,compared to individuals carrying only one or the other.The XPD751C mutation may increase risk of PHC when combined with the environmental factors.
