CAJ | 학술논문

目的了解中国南海部分海域细菌的种类及分布特征.方法采用自制海水采样器对海面下1 m左右的海水进行采样,以浓缩培养基对海水进行增菌,经初步分离后挑取单个菌落至40％甘油中保存,随后转运至临床实验室进一步分离和鉴定.结果在南海部分海域共采集141份海水样品,分离到细菌39种共191株,其中弧菌81株(42.4％),肠杆菌42株(22.0％),非发酵菌24株(12.6％),真菌38株(19.9％),巴斯德菌5株(2.6％),啮氏艾肯菌1株(0.5％).结论南海部分海域海洋细菌以弧菌为主,但肠杆菌与真菌分离率也较高.值得注意的是该海域共分离到9种38株酵母菌,为掌握南海部分海域细菌种类及分布规律提供了重要的本底数据.
목적 료해중국남해부분해역세균적충류급분포특정.방법 채용자제해수채양기대해면하1 m좌우적해수진행채양,이농축배양기대해수진행증균,경초보분리후도취단개균락지40％감유중보존,수후전운지림상실험실진일보분리화감정.결과 재남해부분해역공채집141빈해수양품,분리도세균39충공191주,기중호균81주(42.4％),장간균42주(22.0％),비발효균24주(12.6％),진균38주(19.9％),파사덕균5주(2.6％),교씨애긍균1주(0.5％).결론 남해부분해역해양세균이호균위주,단장간균여진균분리솔야교고.치득주의적시해해역공분리도9충38주효모균,위장악남해부분해역세균충류급분포규률제공료중요적본저수거.
Objective To identify the species and distribution features of marine bacteria in parts of the South China Sea.Methods Seawater samples 1 meter below the sea level were collected by using a selfmade seawater sampler.Then,marine bacteria were enriched with concentrated culture medium,and after initial isolation,single colonies were screened out and were preserved in the 40％ glycerol,and then transported to the laboratory for further identification.Results One hundred and forty-one seawater samples were collected from the region of the South China Sea.From the 141 samples were isolated 39 species (totaling 191 strains),of which 81 strains (42.4％) were Vibrionaceae,42 strains (22.0％) were Enterobacteriaceae,24 strains (12.6％) were nonfermenters,38 strains (19.9％) were fungi,5 strains (2.6％) were Pasteurella and 1 strain (0.5％) was Eikenella.Conclusions Vibrio was the dominant marine bacterium in the South China Sea,however,high isolation rates of Enterobacteriaceae and fungi could also be obtained.More importantly,9 species of saccharomycete (38 strains) were also isolated in our study,which provided important baseline data in the species and distribution features of marine bacteria in the South China Sea.