中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2014年
1期
1-6
,共6页
减压病%高压氧%B细胞淋巴瘤/白血病基因-2蛋白%Bcl-2蛋白相关X蛋白%半胱氨酸天冬氨酸蛋白酶-3蛋白
減壓病%高壓氧%B細胞淋巴瘤/白血病基因-2蛋白%Bcl-2蛋白相關X蛋白%半胱氨痠天鼕氨痠蛋白酶-3蛋白
감압병%고압양%B세포림파류/백혈병기인-2단백%Bcl-2단백상관X단백%반광안산천동안산단백매-3단백
Decompression sickness%Hyperbaric oxygen%B cell lymphoma/lewkmia-2%Bcl-2 associated X protein%Cysteine aspartic acid specific protease-3
目的 探讨高压氧(hyperbaric oxygen,HBO)预处理对减压病大鼠脑组织B细胞淋巴瘤/白血病基因-2蛋白(B cell lymphoma/lewkmia-2,Bcl-2)及相关X蛋白(Bcl-2 associated X protein,Bax)、半胱氨酸天冬氨酸蛋白酶-3蛋白(cysteine aspartic acid specific protease-3,Caspase-3)表达的影响.方法 健康雄性SD大鼠72只,采用数字表法随机分为正常对照组(对照组)、高压氧预处理组(HBO组)、减压病组(DCS组),每组分别设置4个观察时间点(1、5、7、10 d),每个时间点(亚组)6只.建立大鼠减压病模型,HE染色观察减压病后脑组织病理变化,免疫组织化学染色观察各组脑组织Bcl-2、Bax、Caspase-3 的表达.结果 (1) HBO组与DCS组脑组织皮层病情分级为轻度～中度(1～3级).(2)HE染色发现,DCS组大鼠大脑皮层区出现大片疏松区,皮层与海马神经元细胞呈三角形变性坏死,细胞萎缩、体积缩小变性,染色质浓缩甚至碎裂,HBO组神经元变性坏死明显减轻.(3)1、5、7d时,DCS组脑组织皮层Bcl-2阳性细胞数分别为(89.5±15.60)、(176.4±10.22)、(265.52±15.74)个,与对照组(408.67 ±29.57)个相比明显减少,差异均有统计学意义(P＜0.01);HBO组[脑组织皮层阳性细胞数分别为(179.64±12.21)、(253.91±14.00)、(341.15±13.52)个],较DCS组明显增加,差异均有统计学意义(P＜0.05).1、5、7d时,DCS组脑组织皮层Bax阳性细胞数分别为(389.56±18.62)、(337.04±14.85)、(176.41±20.75)个,Caspase-3阳性细胞数分别为(495.64 ±21.03)、(283.04±13.12)、(352.41 ±21.34)个,较对照组1d时[脑组织皮层Bax和Caspase-3分别为(98.64±14.25)、(106.20±14.64)个]明显增加.HBO组Bax阳性细胞数分别为(313.54±21.02)、(253.05±13.60)、(129.03±12.85)个,Caspase-3分别为(429.43±14.08)、(228.05 ±13.60)、(301.02±15.79)个,较DCS组明显减少,差异均有统计学意义(P＜0.05).DCS组Bcl-2/Bax值较对照组明显降低,HBO组Bcl-2/Bax值较DCS组明显升高,差异均有统计学意义(P＜0.05).结论 DCS组大鼠大脑皮层区神经元凋亡1d时最严重,海马区10 d时最明显;HBO预处理可以减轻减压对大鼠脑组织的病理损伤,减轻神经细胞的变性坏死,抑制线粒体途径的细胞凋亡.
目的 探討高壓氧(hyperbaric oxygen,HBO)預處理對減壓病大鼠腦組織B細胞淋巴瘤/白血病基因-2蛋白(B cell lymphoma/lewkmia-2,Bcl-2)及相關X蛋白(Bcl-2 associated X protein,Bax)、半胱氨痠天鼕氨痠蛋白酶-3蛋白(cysteine aspartic acid specific protease-3,Caspase-3)錶達的影響.方法 健康雄性SD大鼠72隻,採用數字錶法隨機分為正常對照組(對照組)、高壓氧預處理組(HBO組)、減壓病組(DCS組),每組分彆設置4箇觀察時間點(1、5、7、10 d),每箇時間點(亞組)6隻.建立大鼠減壓病模型,HE染色觀察減壓病後腦組織病理變化,免疫組織化學染色觀察各組腦組織Bcl-2、Bax、Caspase-3 的錶達.結果 (1) HBO組與DCS組腦組織皮層病情分級為輕度～中度(1～3級).(2)HE染色髮現,DCS組大鼠大腦皮層區齣現大片疏鬆區,皮層與海馬神經元細胞呈三角形變性壞死,細胞萎縮、體積縮小變性,染色質濃縮甚至碎裂,HBO組神經元變性壞死明顯減輕.(3)1、5、7d時,DCS組腦組織皮層Bcl-2暘性細胞數分彆為(89.5±15.60)、(176.4±10.22)、(265.52±15.74)箇,與對照組(408.67 ±29.57)箇相比明顯減少,差異均有統計學意義(P＜0.01);HBO組[腦組織皮層暘性細胞數分彆為(179.64±12.21)、(253.91±14.00)、(341.15±13.52)箇],較DCS組明顯增加,差異均有統計學意義(P＜0.05).1、5、7d時,DCS組腦組織皮層Bax暘性細胞數分彆為(389.56±18.62)、(337.04±14.85)、(176.41±20.75)箇,Caspase-3暘性細胞數分彆為(495.64 ±21.03)、(283.04±13.12)、(352.41 ±21.34)箇,較對照組1d時[腦組織皮層Bax和Caspase-3分彆為(98.64±14.25)、(106.20±14.64)箇]明顯增加.HBO組Bax暘性細胞數分彆為(313.54±21.02)、(253.05±13.60)、(129.03±12.85)箇,Caspase-3分彆為(429.43±14.08)、(228.05 ±13.60)、(301.02±15.79)箇,較DCS組明顯減少,差異均有統計學意義(P＜0.05).DCS組Bcl-2/Bax值較對照組明顯降低,HBO組Bcl-2/Bax值較DCS組明顯升高,差異均有統計學意義(P＜0.05).結論 DCS組大鼠大腦皮層區神經元凋亡1d時最嚴重,海馬區10 d時最明顯;HBO預處理可以減輕減壓對大鼠腦組織的病理損傷,減輕神經細胞的變性壞死,抑製線粒體途徑的細胞凋亡.
목적 탐토고압양(hyperbaric oxygen,HBO)예처리대감압병대서뇌조직B세포림파류/백혈병기인-2단백(B cell lymphoma/lewkmia-2,Bcl-2)급상관X단백(Bcl-2 associated X protein,Bax)、반광안산천동안산단백매-3단백(cysteine aspartic acid specific protease-3,Caspase-3)표체적영향.방법 건강웅성SD대서72지,채용수자표법수궤분위정상대조조(대조조)、고압양예처리조(HBO조)、감압병조(DCS조),매조분별설치4개관찰시간점(1、5、7、10 d),매개시간점(아조)6지.건립대서감압병모형,HE염색관찰감압병후뇌조직병리변화,면역조직화학염색관찰각조뇌조직Bcl-2、Bax、Caspase-3 적표체.결과 (1) HBO조여DCS조뇌조직피층병정분급위경도～중도(1～3급).(2)HE염색발현,DCS조대서대뇌피층구출현대편소송구,피층여해마신경원세포정삼각형변성배사,세포위축、체적축소변성,염색질농축심지쇄렬,HBO조신경원변성배사명현감경.(3)1、5、7d시,DCS조뇌조직피층Bcl-2양성세포수분별위(89.5±15.60)、(176.4±10.22)、(265.52±15.74)개,여대조조(408.67 ±29.57)개상비명현감소,차이균유통계학의의(P＜0.01);HBO조[뇌조직피층양성세포수분별위(179.64±12.21)、(253.91±14.00)、(341.15±13.52)개],교DCS조명현증가,차이균유통계학의의(P＜0.05).1、5、7d시,DCS조뇌조직피층Bax양성세포수분별위(389.56±18.62)、(337.04±14.85)、(176.41±20.75)개,Caspase-3양성세포수분별위(495.64 ±21.03)、(283.04±13.12)、(352.41 ±21.34)개,교대조조1d시[뇌조직피층Bax화Caspase-3분별위(98.64±14.25)、(106.20±14.64)개]명현증가.HBO조Bax양성세포수분별위(313.54±21.02)、(253.05±13.60)、(129.03±12.85)개,Caspase-3분별위(429.43±14.08)、(228.05 ±13.60)、(301.02±15.79)개,교DCS조명현감소,차이균유통계학의의(P＜0.05).DCS조Bcl-2/Bax치교대조조명현강저,HBO조Bcl-2/Bax치교DCS조명현승고,차이균유통계학의의(P＜0.05).결론 DCS조대서대뇌피층구신경원조망1d시최엄중,해마구10 d시최명현;HBO예처리가이감경감압대대서뇌조직적병리손상,감경신경세포적변성배사,억제선립체도경적세포조망.
Objective To investigate the effect of hyperbaric oxygen (HBO) preconditioning on the expressions of B cell lymphoma/lewkmia-2 (Bcl-2),Bcl-2 associated X protein (Bax) and cysteine aspartic acid specific protease-3 (Caspase-3) in the brain tissue of rats with decompression sickness.Methods Seventy-two male healthy Sprague-Dawley rats were randomly divided into the normal control,the HBO preconditioning group (or HBO group),the decompression sickness group (or the DCS group).Four time points (at days 1,5,7 and 10) were set for each subgroup,which consisted of 6 animals.Pathological changes in the brain tissue,following development of the decompression sickness model,were observed with HE staining,and the expressions of Bcl-2,Bax and Caspase-3 were detected with immunohistochemistry in various subgroups.Results (1) For the animals in the HBO and DCS groups,the state of illness was classified as mild and moderate (grade 1-3).(2) HE staining revealed that a large loose area in the rat cerebral cortex of the DCS group was detected,cortical and hippocampus neurons displayed a triangular degeneration and necrosis,cellular atrophy,volume reduction and degeneration,chromatin condensation or even fragmentation.For the animals in the HBO group,necrotic neuron degeneration and necrosis obviously alleviated.(3) At days 1,5 and 7,the expressions of Bcl-2 positive cells for the DCS group were (89.5 ± 15.60),(176.4 ± 10.22)and (265.52± 15.74) respectively,which were significantly lower than those in the control group (408.67 ± 29.57),with statistical significance (P ＜ 0.05),while on the other hand,the expressions of Bcl-2 positive cells for the HBO group were (179.64 ± 12.21),(253.91 ± 14.00) and (341.15 ± 13.52) respectively,which were significantly higher than those of the DCS group,also with statistical significance (P ＜ 0.05).At day 1,5 and 7,the expressions of Bax positive cells for the DCS group were (389.56 ± 18.62),(337.04 ± 14.85) and (176.41 ± 20.75) respectively,and the expressions of Caspase-3 positive cells were (495.64 ± 21.03),(283.04 ± 13.12) and (352.41 ± 21.34),which were obviously higher than those of Bax and Caspase-3 in the control group (98.64 ± 14.25)and (106.20 ± 14.64).For the animals in the HBO group,the expressions of Bax were 313.54 ± 21.02),(253.05 ± 13.60) and (129.03 ± 12.85),and the expressions of Caspase-3 were (429.43 ± 14.08),(228.05 ± 13.60) and(301.02 ± 15.79) respectively,which were significantly lower than those of the control group,also with statistical significance(P ＜0.05).The Bcl-2/Bax values of the DCS group were significantly lower than those of the control group,while the Bcl-2/Bax values of the HBO group were significantly higher that those of the DCS group,with statistical difference (P ＜ 0.05).Conclusions For the animals of the decompression group,apoptosis of neurons in the cortical area was the most serious at day 1,while apoptosis of neurons in the hippocampus at day 10 was the most obvious.HBO preconditioning could alleviate pathological brain lesion induced by pressure,degeneration and necrosis of nerve cells,and at the same time inhibit cell apoptosis through the mitochondrial pathway.
