中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2014年
1期
26-30
,共5页
海水浸泡%开放性骨缺损%骨髓间充质干细胞%高压氧
海水浸泡%開放性骨缺損%骨髓間充質榦細胞%高壓氧
해수침포%개방성골결손%골수간충질간세포%고압양
Seawater immersion%Open bone defect%Bone marrow mesenchymal stem cell%Hyperbaric oxygen
目的 探讨异体骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)经皮移植联合高压氧修复海水浸泡后兔桡骨开放性骨缺损的效果.方法 48只新西兰大白兔,采用数字表法随机分为4组:空白对照组(A组)、BMSCs移植组(B组)、高压氧治疗组(C组)、BMSCs移植联合高压氧治疗组(D组),每组12只.于兔双侧桡骨中段制作15 mm骨缺损,伤口浸泡于人工海水3h,彻底清创后闭合伤口.高压氧治疗于清创术后立即进行,BMSCs移植于清创术后72 h实施.于伤后第4、8、12周分批处死后取材(每组每批4只兔,共8肢).通过影像学分析、组织学及免疫组化观察,比较各组缺损区修复情况.结果 (1)HE染色观察,兔伤后第4、8周时,D组成骨质量最佳,C组优于B组,A组骨缺损区未见骨性修复,由疤痕组织充填;兔伤后第12周时,D组骨缺损区成骨质量最佳,基本是成熟骨组织.(2)免疫组化观察,兔VEGF及TGF-β1主要表达在成骨细胞、软骨细胞及骨细胞胞浆内,在不同时相点有明显的变化.伤后第4周,两者的表达水平均最高;至第8周,其表达较第4周均明显减弱;至第12周,其表达均进一步回落至低水平.伤后第4、8周,两者的表达水平为D组＞C组＞B组＞A组(各组间两两比较,均为P＜0.05).而兔伤后第12周时,A、B、C、D4组间比较差异无统计学意义(P＞0.05).(3)骨痂灰度值测量,兔伤后第12周时,D组骨痂灰度值为211.84 ±4.31,C组为200.31 ±3.08,B组为152.79±4.43,A组为80.88±3.31,组间差异具有统计学意义(P＜0.05).结论 单一异体BMSCs经皮移植或高压氧治疗修复海水浸泡开放性骨缺损效果并不理想.但是,两者联合应用时具有协同作用,成骨作用明显,是修复海水浸泡开放性骨缺损的可行方法.
目的 探討異體骨髓間充質榦細胞(bone marrow mesenchymal stem cells,BMSCs)經皮移植聯閤高壓氧脩複海水浸泡後兔橈骨開放性骨缺損的效果.方法 48隻新西蘭大白兔,採用數字錶法隨機分為4組:空白對照組(A組)、BMSCs移植組(B組)、高壓氧治療組(C組)、BMSCs移植聯閤高壓氧治療組(D組),每組12隻.于兔雙側橈骨中段製作15 mm骨缺損,傷口浸泡于人工海水3h,徹底清創後閉閤傷口.高壓氧治療于清創術後立即進行,BMSCs移植于清創術後72 h實施.于傷後第4、8、12週分批處死後取材(每組每批4隻兔,共8肢).通過影像學分析、組織學及免疫組化觀察,比較各組缺損區脩複情況.結果 (1)HE染色觀察,兔傷後第4、8週時,D組成骨質量最佳,C組優于B組,A組骨缺損區未見骨性脩複,由疤痕組織充填;兔傷後第12週時,D組骨缺損區成骨質量最佳,基本是成熟骨組織.(2)免疫組化觀察,兔VEGF及TGF-β1主要錶達在成骨細胞、軟骨細胞及骨細胞胞漿內,在不同時相點有明顯的變化.傷後第4週,兩者的錶達水平均最高;至第8週,其錶達較第4週均明顯減弱;至第12週,其錶達均進一步迴落至低水平.傷後第4、8週,兩者的錶達水平為D組＞C組＞B組＞A組(各組間兩兩比較,均為P＜0.05).而兔傷後第12週時,A、B、C、D4組間比較差異無統計學意義(P＞0.05).(3)骨痂灰度值測量,兔傷後第12週時,D組骨痂灰度值為211.84 ±4.31,C組為200.31 ±3.08,B組為152.79±4.43,A組為80.88±3.31,組間差異具有統計學意義(P＜0.05).結論 單一異體BMSCs經皮移植或高壓氧治療脩複海水浸泡開放性骨缺損效果併不理想.但是,兩者聯閤應用時具有協同作用,成骨作用明顯,是脩複海水浸泡開放性骨缺損的可行方法.
목적 탐토이체골수간충질간세포(bone marrow mesenchymal stem cells,BMSCs)경피이식연합고압양수복해수침포후토뇨골개방성골결손적효과.방법 48지신서란대백토,채용수자표법수궤분위4조:공백대조조(A조)、BMSCs이식조(B조)、고압양치료조(C조)、BMSCs이식연합고압양치료조(D조),매조12지.우토쌍측뇨골중단제작15 mm골결손,상구침포우인공해수3h,철저청창후폐합상구.고압양치료우청창술후립즉진행,BMSCs이식우청창술후72 h실시.우상후제4、8、12주분비처사후취재(매조매비4지토,공8지).통과영상학분석、조직학급면역조화관찰,비교각조결손구수복정황.결과 (1)HE염색관찰,토상후제4、8주시,D조성골질량최가,C조우우B조,A조골결손구미견골성수복,유파흔조직충전;토상후제12주시,D조골결손구성골질량최가,기본시성숙골조직.(2)면역조화관찰,토VEGF급TGF-β1주요표체재성골세포、연골세포급골세포포장내,재불동시상점유명현적변화.상후제4주,량자적표체수평균최고;지제8주,기표체교제4주균명현감약;지제12주,기표체균진일보회락지저수평.상후제4、8주,량자적표체수평위D조＞C조＞B조＞A조(각조간량량비교,균위P＜0.05).이토상후제12주시,A、B、C、D4조간비교차이무통계학의의(P＞0.05).(3)골가회도치측량,토상후제12주시,D조골가회도치위211.84 ±4.31,C조위200.31 ±3.08,B조위152.79±4.43,A조위80.88±3.31,조간차이구유통계학의의(P＜0.05).결론 단일이체BMSCs경피이식혹고압양치료수복해수침포개방성골결손효과병불이상.단시,량자연합응용시구유협동작용,성골작용명현,시수복해수침포개방성골결손적가행방법.
Objective To explore the effects of HBO combined with percutaneous allogeneic bone marrow mesenchymal stem cells (BMSCs) grafting on the repair of rabbit radius open bone defect following seawater immersion.Methods Forty-eight New Zealand rabbits were randomly divided into 4 groups:the blank control group (group A),the(BMSCs) grafting group (group B),the HBO group (group C),and the HBO combined with BMSCs group (group D).A fifteen-millimeter bone defect was made in the mid-section of radius and then was immersed in the artificial seawater for 3 hours,then the wound was closed with debridement.The animals received HBO therapy the moment after completion of debridement.BMSCs grafting was administered 72 hours after debridement.The animals were sacrificed at week 4,8 and 12 to collect the samples (there were 4 rabbits from each batch of the group,and there were altogether 8 limbs).Repairs of the defect areas were compared between groups by means of radiographic scanning,histologic and immunohistochemical analyses.Results HE staining revealed that osteogenesis in the animals of group D at week 4 and 8 was the best,and group C was superior to group B.In the animals of group A,bone repair was not detected in the defect area,which was filled with scar tissue.At week 12 after injury,osteogenesis in the defect areas for the animals of group D was the best,which was mostly mature bone tissue.(2)Immunohistochemical detection indicated that VEGF and TGF-β1 were mostly expressed in the osteoblastic cells,cartilage cells and bone cell cytoplasm,and significant changes could be noted at different time points.At week 4 after injury,the expressions of VEGF and TGF-β1 all reached peak levels.At week 8,their expressions all decreased significantly,as compared with those at week 4 and 12,their expressions all decreased further to a low level.At week 4 and 8 after injury,the expression levels of VEGF and TGF-β1 for the 4 groups were in the following order:group D ＞ group C ＞ group B ＞ group A.Statistical significance could be seen,when comparisons were made between the groups (P ＜0.05).However,at week 12,no significant differences could detected when comparisons were made between the 4 groups (P ＞ 0.05).(3) Osteotylus measurement revealed that osteotylus grayness for the animals of group D,group C,group B and group A at week 12 after injury was 211.84 ± 4.31,200.31 ± 3.08,152.79 ± 4.43 and 80.88 ± 3.31 respectively,and statistical significance could be noted,when comparisons were made between the groups (P ＜ 0.05).Conclusions The effects of simple percutaneous allogeneic bone marrow mesenchymal stem cells grafting or simple HBO therapy on the repair of open bone defect following seawater immersion were not ideal.However,combined applications of these 2 treatment methods could produce synergistic effects,which could promote osteogenetic process of the affected bone and was an applicable method for the repair of open bone defect following seawater immersion.
