中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2014年
1期
38-42,50
,共6页
陈洪%周春华%李斌%张朝阳%白晓龙
陳洪%週春華%李斌%張朝暘%白曉龍
진홍%주춘화%리빈%장조양%백효룡
动物模型%肾%急性肾损伤%军事医学%肾损伤分子-1%中性粒细胞明胶酶相关脂质运载蛋白%白细胞介素-18
動物模型%腎%急性腎損傷%軍事醫學%腎損傷分子-1%中性粒細胞明膠酶相關脂質運載蛋白%白細胞介素-18
동물모형%신%급성신손상%군사의학%신손상분자-1%중성립세포명효매상관지질운재단백%백세포개소-18
Animal model%Kidney%Acute kidney injury%Military medicine%kidney injury molecule-1%neutrophil gelatinase-associated lipocalin%interleukin-18
目的 探讨肾损伤分子-1(kidney injury molecule-1,KIM-1)、中性粒细胞明胶酶相关脂质运载蛋白(neutrophilgelatinase associated lipocalcin,NGAL)、白细胞介素-18(interteukin-18,IL-18)3种生物学标记物早期诊断大鼠模拟高强度军事训练致急性肾损伤(acute kidney injury,AKI)的效能.方法 雄性12周龄SD大鼠10只为尿检组,构建高强度军事训练AKI模型,留取训练前及训练后不同时间段尿液;48只为血检组,按完全随机方法分为6组,每组8只.按训练前、训练后0、6、12、24、48 h取血清.分别检测尿液、血清KIM-1、NGAL、IL-18及传统诊断指标.结果 训练后即刻,血清、尿液KIM-1、NGAL、IL-18的水平均较对照组明显升高,IL-18升高峰值在2 h,KIM-1、NGAL在6h达峰值,48 h恢复至对照组水平.尿液中KIM-1、NGAL的水平与尿蛋白无相关,与白蛋白、N-乙酰-β-D-氨基葡萄糖甘酶(N-acetyl-β-D-glucosaminidase,NAG)呈正相关.尿IL-18的水平与尿蛋白、白蛋白、NAG呈正相关(P＜0.05).血清中KIM-1、NGAL、IL-18的水平与尿素氮(blood urea nitrogen,Bun)、血清肌酐(serum creatinine,SCr)呈正相关.受试者工作曲线(ROC)分析显示训练后各时间点曲线下面积值(AUC)尿液中KIM-1、NGAL、IL-18均大于尿蛋白、尿白蛋白、尿NAG酶,血清中KIM-1、NGAL、IL-18的AUC值均大于Bun、SCr,血清、尿液相应标志物的AUC值一致.结论 高强度军事训练致AKI后,早期即可探测到血清、尿液KIM-1、NGAL、IL-18的水平升高,诊断效能优于传统诊断标志物,尿液检测可满足诊断要求.
目的 探討腎損傷分子-1(kidney injury molecule-1,KIM-1)、中性粒細胞明膠酶相關脂質運載蛋白(neutrophilgelatinase associated lipocalcin,NGAL)、白細胞介素-18(interteukin-18,IL-18)3種生物學標記物早期診斷大鼠模擬高彊度軍事訓練緻急性腎損傷(acute kidney injury,AKI)的效能.方法 雄性12週齡SD大鼠10隻為尿檢組,構建高彊度軍事訓練AKI模型,留取訓練前及訓練後不同時間段尿液;48隻為血檢組,按完全隨機方法分為6組,每組8隻.按訓練前、訓練後0、6、12、24、48 h取血清.分彆檢測尿液、血清KIM-1、NGAL、IL-18及傳統診斷指標.結果 訓練後即刻,血清、尿液KIM-1、NGAL、IL-18的水平均較對照組明顯升高,IL-18升高峰值在2 h,KIM-1、NGAL在6h達峰值,48 h恢複至對照組水平.尿液中KIM-1、NGAL的水平與尿蛋白無相關,與白蛋白、N-乙酰-β-D-氨基葡萄糖甘酶(N-acetyl-β-D-glucosaminidase,NAG)呈正相關.尿IL-18的水平與尿蛋白、白蛋白、NAG呈正相關(P＜0.05).血清中KIM-1、NGAL、IL-18的水平與尿素氮(blood urea nitrogen,Bun)、血清肌酐(serum creatinine,SCr)呈正相關.受試者工作麯線(ROC)分析顯示訓練後各時間點麯線下麵積值(AUC)尿液中KIM-1、NGAL、IL-18均大于尿蛋白、尿白蛋白、尿NAG酶,血清中KIM-1、NGAL、IL-18的AUC值均大于Bun、SCr,血清、尿液相應標誌物的AUC值一緻.結論 高彊度軍事訓練緻AKI後,早期即可探測到血清、尿液KIM-1、NGAL、IL-18的水平升高,診斷效能優于傳統診斷標誌物,尿液檢測可滿足診斷要求.
목적 탐토신손상분자-1(kidney injury molecule-1,KIM-1)、중성립세포명효매상관지질운재단백(neutrophilgelatinase associated lipocalcin,NGAL)、백세포개소-18(interteukin-18,IL-18)3충생물학표기물조기진단대서모의고강도군사훈련치급성신손상(acute kidney injury,AKI)적효능.방법 웅성12주령SD대서10지위뇨검조,구건고강도군사훈련AKI모형,류취훈련전급훈련후불동시간단뇨액;48지위혈검조,안완전수궤방법분위6조,매조8지.안훈련전、훈련후0、6、12、24、48 h취혈청.분별검측뇨액、혈청KIM-1、NGAL、IL-18급전통진단지표.결과 훈련후즉각,혈청、뇨액KIM-1、NGAL、IL-18적수평균교대조조명현승고,IL-18승고봉치재2 h,KIM-1、NGAL재6h체봉치,48 h회복지대조조수평.뇨액중KIM-1、NGAL적수평여뇨단백무상관,여백단백、N-을선-β-D-안기포도당감매(N-acetyl-β-D-glucosaminidase,NAG)정정상관.뇨IL-18적수평여뇨단백、백단백、NAG정정상관(P＜0.05).혈청중KIM-1、NGAL、IL-18적수평여뇨소담(blood urea nitrogen,Bun)、혈청기항(serum creatinine,SCr)정정상관.수시자공작곡선(ROC)분석현시훈련후각시간점곡선하면적치(AUC)뇨액중KIM-1、NGAL、IL-18균대우뇨단백、뇨백단백、뇨NAG매,혈청중KIM-1、NGAL、IL-18적AUC치균대우Bun、SCr,혈청、뇨액상응표지물적AUC치일치.결론 고강도군사훈련치AKI후,조기즉가탐측도혈청、뇨액KIM-1、NGAL、IL-18적수평승고,진단효능우우전통진단표지물,뇨액검측가만족진단요구.
Objective To investigate early diagnostic efficacy of the kidney injury molecule-1 (KIM-1),neutrophilgelatinase associated lipocalcin (NGAL) and interleukin-18 (IL-18) in acute kidney injury (AKI) induced by high intensity military training through the rat model.Methods Ten male SD rats,12 weeks old,were used as the urine detection group to develop the animal model of AKI induced by high intensity military training.Urine samples were taken at different time points both before and after training.Another 48 SD rats were designated as the blood detection group and serum samples were collected before training and at hours 0,6,24 and 48 after training.Changes in the urine and serum concentration of KIM-1,NGAL,IL-18 and routine markers were detected respectively.Results The urine and serum KIM-1,NGAL and IL-18 all increased significantly the moment after termination of training,as compared with those of the control group (P＜0.05).IL-18 reached peak at hour 2,KIM-1 and NGAL reached peak at hour 6,and then they decreased to baselines in 48 hours.Urine KIM-1 and NGAL were not correlated with urine protein,while they were positively correlated with urine albumin (UA) and NAG.Urine IL-18 was positively correlated with urine protein,urine albumin (UA) and NAG(P ＜0.05).Serum KIM-1,NGAL and IL-18 were positively correlated with serum Cr and Bun.ROC analysis showed that the levels of urine KIM-1,NGAL and uIL-18 in the area under the curve (AUC) at various time points after training were all higher than those of urine protein,UA and NAG.The AUC value of serum KIM-1,NGAL and IL-18 were all higher than those of SCr and Bun at any time points after training,and it was corresponding to the AUC value of the serum and urine biomarkers.Conclusions Following acquired acute kidney injury induced by high intensity military training,higher levels of serum and urine KIM-1,NGAL and IL-18 could be detected immediately after training.The diagnostic efficiency was superior to those traditional markers.Urine detection could meet the needs of diagnosis.
