CAJ | 학술논문

目的探讨高压氧(HBO)暴露对药物致卵巢早衰大鼠的影响.方法 50只SPF级雌性Wistar大鼠按数字表法随机分为5组:正常组、模型组、对照组、雌孕激素替代治疗(HRT)组和HBO组,每组10只.除了正常组外,其余各组均给予雷公藤多甙片溶液灌胃,建立卵巢早衰模型.正常组和模型组大鼠在灌胃14 d后分别留取血清,处死并留取卵巢和子宫.其余各组给予不同干预,于造模后40 d留取血清,处死并留取卵巢和子宫.同批检测血清雌二醇(E2)水平,并对其动情周期、子宫和卵巢的脏器指数、子宫内膜厚度、卵巢面积等进行观察分析.结果灌药后,大鼠动情周期发生紊乱,表现形式多样;各组给予干预后,其动情周期均不同程度逐渐恢复正常,对照组动情周期仍表现紊乱;模型组血清E2水平、内膜厚度以及脏器指数均低于正常组,单位卵巢面积内卵泡数较正常组少(P＜0.05),闭锁卵泡数较正常组多(P＜0.05);卵巢面积较正常组小;干预40 d后,HBO组血清E2水平较HRT组和对照组均高(均P＜0.05).HBO组和HRT组子宫内膜厚度和子宫脏器指数较对照组高,HBO组较HRT组高(均P ＜0.05).HBO组单位卵巢面积、卵泡数比HRT组和对照组多,但仅与对照组的差异有统计学意义(P＜0.05).HBO组和HRT组闭锁卵泡数比对照组少(P＜0.05).结论雷公藤多甙片灌胃法建立大鼠卵巢早衰模型是可行的;HBO暴露可以改善卵巢功能,使E2水平升高,增加子宫、卵巢的重量及子宫内膜厚度.
목적 탐토고압양(HBO)폭로대약물치란소조쇠대서적영향.방법 50지SPF급자성Wistar대서안수자표법수궤분위5조:정상조、모형조、대조조、자잉격소체대치료(HRT)조화HBO조,매조10지.제료정상조외,기여각조균급여뢰공등다대편용액관위,건립란소조쇠모형.정상조화모형조대서재관위14 d후분별류취혈청,처사병류취란소화자궁.기여각조급여불동간예,우조모후40 d류취혈청,처사병류취란소화자궁.동비검측혈청자이순(E2)수평,병대기동정주기、자궁화란소적장기지수、자궁내막후도、란소면적등진행관찰분석.결과 관약후,대서동정주기발생문란,표현형식다양;각조급여간예후,기동정주기균불동정도축점회복정상,대조조동정주기잉표현문란;모형조혈청E2수평、내막후도이급장기지수균저우정상조,단위란소면적내란포수교정상조소(P＜0.05),폐쇄란포수교정상조다(P＜0.05);란소면적교정상조소;간예40 d후,HBO조혈청E2수평교HRT조화대조조균고(균P＜0.05).HBO조화HRT조자궁내막후도화자궁장기지수교대조조고,HBO조교HRT조고(균P ＜0.05).HBO조단위란소면적、란포수비HRT조화대조조다,단부여대조조적차이유통계학의의(P＜0.05).HBO조화HRT조폐쇄란포수비대조조소(P＜0.05).결론 뢰공등다대편관위법건립대서란소조쇠모형시가행적;HBO폭로가이개선란소공능,사E2수평승고,증가자궁、란소적중량급자궁내막후도.
Objective To investigate the effects of hyperbaric oxygen (HBO) on rats with premature ovary failure (POF) induced by triptergium wilfordii polyglycisidium (TWP).Methods Fifty female SPF rats were randomly divided into 5 groups:the normal group,the model group,the control group,the hormone replacement therapy (HRT) group and the HBO group,each consisiting of 10 animals.Except the normal group,all the other groups were given orally triptegium wilfordii polyglycisidium (TWP) to establish premature ovary failure model.Fourteen days after intragastric administration of TWP,serum samples from the normal and model groups were taken,then the animals were sacrificed and uterus and ovary were collected for detection.The animals in other groups were treated differently,and 40 days after establishment of the model,serum samples were taken,then the animals were also sacrificed and uterus and ovary were collected for the detection of related parameters.Serum estradiol (E2) level,changes in estrous cycle,uterine and ovarian weight,endometrial thickness and ovarian size were observed and analyzed.Results After intragastric administration of the drug,estrous cycle of rats was disturbed in various forms.Following treatment with HRT and HBO,estrous cycle all gradually recovered to a more or less extent,while estrous cycle of the rats in the control group still remained in a state of disorder.Serum E2 level,endometrial thickness and organ weight index in the model group were significantly lower than those of the normal group (P ＜ 0.05).Besides,follicular number in ovary unit area was significantly less than that of the normal group,while atretic follicular number was obviously more than that of the normal group (P ＜ 0.05).Ovary area in the model group was smaller than that of the normal group (P ＞ 0.05).Forty days after intervention,serum E2 level in the HBO group was higher than that of the control group and the HRT group (P ＜ 0.05).Endometrial thickness and uterus weight indexes in the HBO group and the HRT group were significantly higher,as compared with those of the control group (P ＜ 0.05),and the levels of the HBO group were even higher than those of the HRT group (P ＜ 0.05).Ovary unit area and follicular number of the HRT group were either larger or greater than those of the HRT group and the control group,however,statistical significance could only be noted,when they compared with those of the control group (P ＜ 0.05).Atretic follicular number of the HBO group and the HRT group was significantly less than that of the control group (P ＜ 0.05).Conclusions It was feasible to establish the rat model of POF by intragastric administration of TWP.HBO could improve the function of ovary in rats with premature ovary failure,elevate serum E2 level,increase uterus and ovary weight and endometrial thickness as well.