中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2014年
3期
150-152
,共3页
邱丽丽%王春莲%郝娟%王培嵩%蔡慧慧%万金娥%谷健
邱麗麗%王春蓮%郝娟%王培嵩%蔡慧慧%萬金娥%穀健
구려려%왕춘련%학연%왕배숭%채혜혜%만금아%곡건
高压氧%卵巢早衰%骨质疏松%激素替代治疗%骨代谢%骨碱性磷酸酶%抗酒石酸酸性磷酸酶
高壓氧%卵巢早衰%骨質疏鬆%激素替代治療%骨代謝%骨堿性燐痠酶%抗酒石痠痠性燐痠酶
고압양%란소조쇠%골질소송%격소체대치료%골대사%골감성린산매%항주석산산성린산매
Hyperbaric oxygen%Premature ovary failure%Osteoporosis%Hormone replacement therapy%Bone metabolism%Bone alkaline phosphatase%Tartrate-resistant acid phospatase
目的 探讨卵巢早衰大鼠骨代谢变化及高压氧(HBO)暴露的影响.方法 将40只SPF级雌性Wistar大鼠按数字表法随机分为空白组、造模空白组、雌孕激素替代治疗(HRT)组、HBO组,每组10只.除空白组外,其余各组均给予雷公藤多甙片溶液灌胃,建立卵巢早衰模型.然后,HRT组给予雌孕激素灌胃治疗,HBO组给予HBO治疗及等量生理盐水灌胃,空白组及造模空白组给予等量生理盐水灌胃.观察各组血清骨碱性磷酸酶(BALP)、抗酒石酸酸性磷酸酶(TRACP)的变化.结果 与空白组相比,造模空白组BALP[(245.24±25.21) ng/L]显著降低(P＜0.01),TRACP[(3 453.69±413.82)pg/L]则显著升高(P＜0.01);HRT组和HBO组的BALP[分别为(342.16±18.68)、(306.64±22.96)ng/L]均高于造模空白组,TRACP[(1 735.48±94.56)、(1 889.63 ±70.81) pg/L]均低于造模空白组[(3 453.69±413.82) pg/L],差异均有统计学意义(P＜0.01).结论 HBO暴露可影响卵巢早衰大鼠骨代谢,对促进骨形成的BALP有增强作用,对促进骨吸收的TRACP有抑制作用.
目的 探討卵巢早衰大鼠骨代謝變化及高壓氧(HBO)暴露的影響.方法 將40隻SPF級雌性Wistar大鼠按數字錶法隨機分為空白組、造模空白組、雌孕激素替代治療(HRT)組、HBO組,每組10隻.除空白組外,其餘各組均給予雷公籐多甙片溶液灌胃,建立卵巢早衰模型.然後,HRT組給予雌孕激素灌胃治療,HBO組給予HBO治療及等量生理鹽水灌胃,空白組及造模空白組給予等量生理鹽水灌胃.觀察各組血清骨堿性燐痠酶(BALP)、抗酒石痠痠性燐痠酶(TRACP)的變化.結果 與空白組相比,造模空白組BALP[(245.24±25.21) ng/L]顯著降低(P＜0.01),TRACP[(3 453.69±413.82)pg/L]則顯著升高(P＜0.01);HRT組和HBO組的BALP[分彆為(342.16±18.68)、(306.64±22.96)ng/L]均高于造模空白組,TRACP[(1 735.48±94.56)、(1 889.63 ±70.81) pg/L]均低于造模空白組[(3 453.69±413.82) pg/L],差異均有統計學意義(P＜0.01).結論 HBO暴露可影響卵巢早衰大鼠骨代謝,對促進骨形成的BALP有增彊作用,對促進骨吸收的TRACP有抑製作用.
목적 탐토란소조쇠대서골대사변화급고압양(HBO)폭로적영향.방법 장40지SPF급자성Wistar대서안수자표법수궤분위공백조、조모공백조、자잉격소체대치료(HRT)조、HBO조,매조10지.제공백조외,기여각조균급여뢰공등다대편용액관위,건립란소조쇠모형.연후,HRT조급여자잉격소관위치료,HBO조급여HBO치료급등량생리염수관위,공백조급조모공백조급여등량생리염수관위.관찰각조혈청골감성린산매(BALP)、항주석산산성린산매(TRACP)적변화.결과 여공백조상비,조모공백조BALP[(245.24±25.21) ng/L]현저강저(P＜0.01),TRACP[(3 453.69±413.82)pg/L]칙현저승고(P＜0.01);HRT조화HBO조적BALP[분별위(342.16±18.68)、(306.64±22.96)ng/L]균고우조모공백조,TRACP[(1 735.48±94.56)、(1 889.63 ±70.81) pg/L]균저우조모공백조[(3 453.69±413.82) pg/L],차이균유통계학의의(P＜0.01).결론 HBO폭로가영향란소조쇠대서골대사,대촉진골형성적BALP유증강작용,대촉진골흡수적TRACP유억제작용.
Objective To investigate changes of bone metabolism in rats with premature ovary failure (POF) and the effects of hyperbaric oxygenation (HBO) on bone metabolism.Methods Forty SPF Wistar rats were randomly divided into 4 groups:the blank control group,the model control group,the hormone replacement therapy (HRT) group and the HBO group,each consisiting of 10 animals.Except the blank control group,all the other groups were orally given triptegium wilfordii polyglycisidium (TWP) solution to establish premature ovary failure model.And then,rats in the HRT group were given orally estrogen and progestin.Rats in the HBO group were treated with HBO and the same dosage of normal saline.Rats in the control group and the model control group were given orally the same dosage of normal saline.Then,changes of serum bone alkaline phosphatase (BALP) and tartrate-resistant acid phospatase (TRACP) in different groups were observed accordingly.Results When compared with that of the blank control group,the level of BALP in the model control group[(245.24 ± 25.21) ng/L] was decreased significantly (P ＜ 0.01),while the level of TRACP[(3453.69 ± 413.82) pg/L] was increased markedly (P ＜ 0.01).BALP levels of the HRT group [(342.16 ± 18.68) ng/L] and the HBO group [(306.64 ± 22.96) ng/L] were all higher than those of the model control group,while TRACP levels[(1735.48 ± 94.56) and (1889.63 ± 70.81) pg/L] were all lower than those of the model control group [(3453.69 ± 413.82)pg/L],with statistical significance (P ＜ 0.01).Conclusions HBO could affect bone metabolism in rats with premature ovary failure,had enhancing effect on BALP in bone formation,and at the same time had certain suppressing effect on TRACP in its role of bone absorption.
