CAJ | 학술논문

目的观察高压氧暴露对肾上腺素导致的大鼠内耳损伤、听力下降的影响,以及相关激素的改变情况,评价高压氧治疗对听力及激素水平的影响.方法 24只大鼠按数字表法随机分为3组:空白对照组、肾上腺素组、肾上腺素+高压氧组.肾上腺素组、肾上腺素+高压氧组连续5d经中耳注入肾上腺素引起听力下降,对照组使用生理盐水.给药结束后肾上腺素+高压氧组给予高压氧治疗,1次/d,连续3d,另2组自然恢复3d.通过听性脑干反应(auditory brainstem response,ABR)检测大鼠听阈变化.实验结束处死动物,检测血清激素水平.结果中耳给予肾上腺素造成大鼠听阈升高,肾上腺素+高压氧组大鼠最终听阈[(26.01 ±7.95) dB]低于肾上腺素组[(39.69 ±8.26) dB],差异有统计学意义(P＜0.05).对照组皮质醇水平最低,肾上腺素+高压氧组次之,肾上腺素组最高,3组之间比较差异均有统计学意义(P＜0.05).结论高压氧治疗减轻肾上腺素造成的大鼠听力损害,皮质醇含量与听力损害程度密切相关.
목적 관찰고압양폭로대신상선소도치적대서내이손상、은력하강적영향,이급상관격소적개변정황,평개고압양치료대은력급격소수평적영향.방법 24지대서안수자표법수궤분위3조:공백대조조、신상선소조、신상선소+고압양조.신상선소조、신상선소+고압양조련속5d경중이주입신상선소인기은력하강,대조조사용생리염수.급약결속후신상선소+고압양조급여고압양치료,1차/d,련속3d,령2조자연회복3d.통과은성뇌간반응(auditory brainstem response,ABR)검측대서은역변화.실험결속처사동물,검측혈청격소수평.결과 중이급여신상선소조성대서은역승고,신상선소+고압양조대서최종은역[(26.01 ±7.95) dB]저우신상선소조[(39.69 ±8.26) dB],차이유통계학의의(P＜0.05).대조조피질순수평최저,신상선소+고압양조차지,신상선소조최고,3조지간비교차이균유통계학의의(P＜0.05).결론 고압양치료감경신상선소조성적대서은력손해,피질순함량여은력손해정도밀절상관.
Objective To observe the effects of hyperbaric oxygen (HBO) on the damage of inner ear and hearing loss induced by adrenaline and also to monitor changes in hormone levels related with stress,so as to evaluate effects of HBO on hearing damage and related stressors.Methods Twenty-four male Wistar rats were randomly divided into 3 groups:the blank control group,the adrenaline group and the adrenaline plus HBO group.Infusion of adrenaline through the access of middle ear for a succession of 5 days till hearing loss was induced,while the animals in the control group were only given physiologic saline.After completion of medication,the animals in the HBO group received HBO therapy 1 session / a day for 3 consecutive days,and the animals in the other 2 groups were left untreated for natural recovery.Changes in hearing thresholds in rats were detected through monitoring of evoked auditory brainstem response (ABR).After termination of the experiment,the animals were sacrificed for detection of serum hormone levels.Results The administration of adrenaline through the access of middle ear could elevate the hearing threshold of the rats.The final hearing threshold of the rats in the HBO group [(26.01 ± 7.95) dB] was lower than that of the adrenaline group [(39.69 ± 8.26) dB] (P ＜ 0.05).Cortisol level in the animals of the control group was the lowest,followed by the animals in the HBO group.The cortisol level in the animals of the model group was the highest.Conclusions HBO therapy could alleviate hearing damage of the rats induced by adrenaline.Cortical levels were closely associated with the seriousness of hearing damage.