中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2014年
4期
238-241
,共4页
范祯祯%刘志艳%蔡宏斌%梁成%赵翀翀%张旭东%张翠杰%张兰芳%葛朝明
範禎禎%劉誌豔%蔡宏斌%樑成%趙翀翀%張旭東%張翠傑%張蘭芳%葛朝明
범정정%류지염%채굉빈%량성%조충충%장욱동%장취걸%장란방%갈조명
高压氧%内皮素-1%内皮型一氧化氮合酶%实时荧光定量PCR
高壓氧%內皮素-1%內皮型一氧化氮閤酶%實時熒光定量PCR
고압양%내피소-1%내피형일양화담합매%실시형광정량PCR
Hyperbaric oxygen%Basal artery%Endothelin-1%Endothelial nitric oxide synthase%Real-time-FQ-PCR
目的 检测高压氧(hyperbaric oxygen,HBO)对实验兔颈内动脉和基底动脉内皮素-1(ET-1)及内皮型一氧化氮合酶(eNOS) mRNA表达的影响,探讨HBO暴露致脑血管前循环收缩、后循环扩张的可能机制.方法 健康成年新西兰白兔24只,按数字表法随机分为实验组(n=12)和对照组(n=12).实验组每日持续暴露于0.22 MPa HBO下60 min,连续3d;对照组正常饲养,不予任何处理.实时荧光定量PCR法(FQ-RT-PCR)检测ET-1、eNOS mRNA在2组颈内动脉和基底动脉的表达水平.结果 ET-1、eNOS mRNA在实验兔颈内动脉和基底动脉均有微量表达.与对照组相比,HBO可使实验组颈内动脉ET-1 mRNA表达明显增高[(1.679 ±0.097) vs (1.000±0.017)],eNOS mRNA表达明显下降[(0.571 ±0.039) vs (1.000 ±0.023)];基底动脉ET-1 mRNA表达明显下降[(0.565 ±0.069) vs(1.000±0.034)],eNOS mRNA表达明显增高[(1.667 ±0.134) vs(1.000±0.014)],差异均有统计学意义(P＜0.01).结论 HBO可通过调节ET-1、eNOS mRNA在颈内动脉和基底动脉的相对表达量而发挥收缩前循环、扩张后循环的作用.
目的 檢測高壓氧(hyperbaric oxygen,HBO)對實驗兔頸內動脈和基底動脈內皮素-1(ET-1)及內皮型一氧化氮閤酶(eNOS) mRNA錶達的影響,探討HBO暴露緻腦血管前循環收縮、後循環擴張的可能機製.方法 健康成年新西蘭白兔24隻,按數字錶法隨機分為實驗組(n=12)和對照組(n=12).實驗組每日持續暴露于0.22 MPa HBO下60 min,連續3d;對照組正常飼養,不予任何處理.實時熒光定量PCR法(FQ-RT-PCR)檢測ET-1、eNOS mRNA在2組頸內動脈和基底動脈的錶達水平.結果 ET-1、eNOS mRNA在實驗兔頸內動脈和基底動脈均有微量錶達.與對照組相比,HBO可使實驗組頸內動脈ET-1 mRNA錶達明顯增高[(1.679 ±0.097) vs (1.000±0.017)],eNOS mRNA錶達明顯下降[(0.571 ±0.039) vs (1.000 ±0.023)];基底動脈ET-1 mRNA錶達明顯下降[(0.565 ±0.069) vs(1.000±0.034)],eNOS mRNA錶達明顯增高[(1.667 ±0.134) vs(1.000±0.014)],差異均有統計學意義(P＜0.01).結論 HBO可通過調節ET-1、eNOS mRNA在頸內動脈和基底動脈的相對錶達量而髮揮收縮前循環、擴張後循環的作用.
목적 검측고압양(hyperbaric oxygen,HBO)대실험토경내동맥화기저동맥내피소-1(ET-1)급내피형일양화담합매(eNOS) mRNA표체적영향,탐토HBO폭로치뇌혈관전순배수축、후순배확장적가능궤제.방법 건강성년신서란백토24지,안수자표법수궤분위실험조(n=12)화대조조(n=12).실험조매일지속폭로우0.22 MPa HBO하60 min,련속3d;대조조정상사양,불여임하처리.실시형광정량PCR법(FQ-RT-PCR)검측ET-1、eNOS mRNA재2조경내동맥화기저동맥적표체수평.결과 ET-1、eNOS mRNA재실험토경내동맥화기저동맥균유미량표체.여대조조상비,HBO가사실험조경내동맥ET-1 mRNA표체명현증고[(1.679 ±0.097) vs (1.000±0.017)],eNOS mRNA표체명현하강[(0.571 ±0.039) vs (1.000 ±0.023)];기저동맥ET-1 mRNA표체명현하강[(0.565 ±0.069) vs(1.000±0.034)],eNOS mRNA표체명현증고[(1.667 ±0.134) vs(1.000±0.014)],차이균유통계학의의(P＜0.01).결론 HBO가통과조절ET-1、eNOS mRNA재경내동맥화기저동맥적상대표체량이발휘수축전순배、확장후순배적작용.
Objective To detect the effects of hyperbaric oxygen (HBO) on the expressions of endothelin-1 (ET-1) and endothelial nitric oxide synthase (eNOS) mRNA in the internal carotid artery (ICA) and basal artery (BA) of rabbits,and also to explore the mechanism involved in the constriction of the cerebrovascular anterior circulation and dilation of the cerebrovascular posterior circulation.Methods Twenty-four healthy adult New Zealand rabbits were randomly divided into 2 groups:the experimental group and the control group,each consisting of 12 animals.The rabbits in the experimental group were exposed to HB0 at 2 ATA for 60 minutes a day for 3 successive days,while the rabbits in the control group were normally fed without any treatment.Real-time-FQ-PCR was used to detect expression levels of ET-1 and eNOS mRNA in both ICA and BA of the 2 groups.Results Slight expressions of ET-1 and eNOS mRNA could be detected in ICA and BA of the experimental group.Significant increase in the expression of ET-1 mRNA and marked decrease in the expression of eNOS mRNA in ICA could be detected in the experimental group (1.679 ± 0.097),as compared with that of the control group (1.000 ± 0.017).At the same time,HBO exposure could significantly induce the expression of ET-1 mRNA and marked increase in the expression of eNOS mRNA in BA,with statistical significance (P ＜ 0.01).Conclusions Through regulation of the expression amount of ET-1 and eNOS mRNA in both ICA and BA,HBO could play a role in the contraction of cerebrovascular anterior circulation and dilation of cerebrovascular posterior circulation.
