目的 观察高压氧(hyperbaric oxygen,HBO)预处理对模拟海拔7000m急性缺氧大鼠血流动力学和白细胞介素-6(IL-6)、IL-10、肿瘤坏死因子-α(TNF-α)的影响.方法 SD大鼠50只按随机数字表法分为6组.HBO组(Ⅰ、Ⅱ、Ⅲ)给予HBO预处理7d,对照组(Ⅰ、Ⅱ、Ⅲ)未给予任何处理.HBO Ⅰ组(n=10)、HBOⅡ组(n=10)、HBO Ⅲ组(n=10)HBO预处理后和对照Ⅰ组(n=6)、对照Ⅱ组(n=7)、对照Ⅲ组(n=7)分别在海拔7000m低压舱内停留12、24h和72 h,各组大鼠不同时程在低压氧舱内用10％氨基甲酸乙酯腹腔麻醉,尔后分别经右颈外静脉插入心导管至右心室、肺动脉,用多道生理记录仪连续描记5 min肺动脉压力曲线,测定肺动脉压力(PAP)、左右室收缩压(RVSP、LVSD)和舒张压(RVEDP、LVEDP)、右室等容收缩期心室内压力上升最大速率和右室等容舒张期心室内压力下降最大速率(±dp/dtmax)等血流动力学指标,并采血检测血清白细胞介素-6(IL-6)、IL-10和肿瘤坏死因子-α(TNF-α)、超氧化物歧化酶(SOD)和丙二醛(MDA).结果 (1)HBO Ⅰ组较对照Ⅰ组RVEDP降低,HBOⅢ组较对照Ⅲ组mPAP、RVEDP、-dp/dtmax降低,差异均有统计学意义(P＜0.05);与HBO Ⅰ组比较,HBO Ⅱ组mPAP、±dp/dtmax降低(P＜0.05、P＜0.01),HBO Ⅲ组RVSP(P ＜0.05)、RVEDP、±dp/dtmax、mPAP降低,差异有统计学意义(P＜0.01);与HBO Ⅱ组比较,HBO Ⅲ组RVEDP降低,差异有统计学意义(P＜0.01).(2) HBO Ⅰ组LVEDP较对照Ⅰ组降低,HBO Ⅲ组LVEDP较对照组Ⅲ组降低,差异均有统计学意义(P ＜0.05、P＜0.01);与HBOⅠ组比较,HBO Ⅱ组LVEDP升高(P＜0.05),HBO Ⅲ组LVSP、LVEDP升高(P ＜0.05、0.01),差异均有统计学意义(P＜0.05、P＜0.01).(3) HBO Ⅰ组与对照Ⅰ组比较,SOD增高,IL-6、IL-10、TNF-α降低,差异有统计学意义(P＜0.05).HBO Ⅱ组与对照Ⅱ组比较,SOD增高,IL-6、IL-10、TNF-α降低,差异有统计学意义(P＜0.05或P＜0.01).HBO Ⅲ组与对照Ⅲ组比较,SOD增高,MDA、IL-6、IL-10、TNF-α降低,差异有统计学意义(P＜0.05).与HBO Ⅰ组比较,HBO Ⅱ组IL-6、TNF-α升高,差异有统计学意义(均P＜0.05);HBO Ⅲ组SOD降低,IL-6、TNF-α升高,差异有统计学意义(均P＜0.05),IL-10、MDA升高,差异有统计学意义(P＜0.01);与HBO Ⅱ组比较,HBO Ⅲ组SOD降低,IL-6、IL-10、TNF-α、MDA升高,差异均有统计学意义(均P＜0.05).结论 HBO预处理可改善缺氧机体血流动力学,使机体抗氧化能力增强,提高心室功能和低氧耐受能力.
目的 觀察高壓氧(hyperbaric oxygen,HBO)預處理對模擬海拔7000m急性缺氧大鼠血流動力學和白細胞介素-6(IL-6)、IL-10、腫瘤壞死因子-α(TNF-α)的影響.方法 SD大鼠50隻按隨機數字錶法分為6組.HBO組(Ⅰ、Ⅱ、Ⅲ)給予HBO預處理7d,對照組(Ⅰ、Ⅱ、Ⅲ)未給予任何處理.HBO Ⅰ組(n=10)、HBOⅡ組(n=10)、HBO Ⅲ組(n=10)HBO預處理後和對照Ⅰ組(n=6)、對照Ⅱ組(n=7)、對照Ⅲ組(n=7)分彆在海拔7000m低壓艙內停留12、24h和72 h,各組大鼠不同時程在低壓氧艙內用10％氨基甲痠乙酯腹腔痳醉,爾後分彆經右頸外靜脈插入心導管至右心室、肺動脈,用多道生理記錄儀連續描記5 min肺動脈壓力麯線,測定肺動脈壓力(PAP)、左右室收縮壓(RVSP、LVSD)和舒張壓(RVEDP、LVEDP)、右室等容收縮期心室內壓力上升最大速率和右室等容舒張期心室內壓力下降最大速率(±dp/dtmax)等血流動力學指標,併採血檢測血清白細胞介素-6(IL-6)、IL-10和腫瘤壞死因子-α(TNF-α)、超氧化物歧化酶(SOD)和丙二醛(MDA).結果 (1)HBO Ⅰ組較對照Ⅰ組RVEDP降低,HBOⅢ組較對照Ⅲ組mPAP、RVEDP、-dp/dtmax降低,差異均有統計學意義(P＜0.05);與HBO Ⅰ組比較,HBO Ⅱ組mPAP、±dp/dtmax降低(P＜0.05、P＜0.01),HBO Ⅲ組RVSP(P ＜0.05)、RVEDP、±dp/dtmax、mPAP降低,差異有統計學意義(P＜0.01);與HBO Ⅱ組比較,HBO Ⅲ組RVEDP降低,差異有統計學意義(P＜0.01).(2) HBO Ⅰ組LVEDP較對照Ⅰ組降低,HBO Ⅲ組LVEDP較對照組Ⅲ組降低,差異均有統計學意義(P ＜0.05、P＜0.01);與HBOⅠ組比較,HBO Ⅱ組LVEDP升高(P＜0.05),HBO Ⅲ組LVSP、LVEDP升高(P ＜0.05、0.01),差異均有統計學意義(P＜0.05、P＜0.01).(3) HBO Ⅰ組與對照Ⅰ組比較,SOD增高,IL-6、IL-10、TNF-α降低,差異有統計學意義(P＜0.05).HBO Ⅱ組與對照Ⅱ組比較,SOD增高,IL-6、IL-10、TNF-α降低,差異有統計學意義(P＜0.05或P＜0.01).HBO Ⅲ組與對照Ⅲ組比較,SOD增高,MDA、IL-6、IL-10、TNF-α降低,差異有統計學意義(P＜0.05).與HBO Ⅰ組比較,HBO Ⅱ組IL-6、TNF-α升高,差異有統計學意義(均P＜0.05);HBO Ⅲ組SOD降低,IL-6、TNF-α升高,差異有統計學意義(均P＜0.05),IL-10、MDA升高,差異有統計學意義(P＜0.01);與HBO Ⅱ組比較,HBO Ⅲ組SOD降低,IL-6、IL-10、TNF-α、MDA升高,差異均有統計學意義(均P＜0.05).結論 HBO預處理可改善缺氧機體血流動力學,使機體抗氧化能力增彊,提高心室功能和低氧耐受能力.
목적 관찰고압양(hyperbaric oxygen,HBO)예처리대모의해발7000m급성결양대서혈류동역학화백세포개소-6(IL-6)、IL-10、종류배사인자-α(TNF-α)적영향.방법 SD대서50지안수궤수자표법분위6조.HBO조(Ⅰ、Ⅱ、Ⅲ)급여HBO예처리7d,대조조(Ⅰ、Ⅱ、Ⅲ)미급여임하처리.HBO Ⅰ조(n=10)、HBOⅡ조(n=10)、HBO Ⅲ조(n=10)HBO예처리후화대조Ⅰ조(n=6)、대조Ⅱ조(n=7)、대조Ⅲ조(n=7)분별재해발7000m저압창내정류12、24h화72 h,각조대서불동시정재저압양창내용10％안기갑산을지복강마취,이후분별경우경외정맥삽입심도관지우심실、폐동맥,용다도생리기록의련속묘기5 min폐동맥압력곡선,측정폐동맥압력(PAP)、좌우실수축압(RVSP、LVSD)화서장압(RVEDP、LVEDP)、우실등용수축기심실내압력상승최대속솔화우실등용서장기심실내압력하강최대속솔(±dp/dtmax)등혈류동역학지표,병채혈검측혈청백세포개소-6(IL-6)、IL-10화종류배사인자-α(TNF-α)、초양화물기화매(SOD)화병이철(MDA).결과 (1)HBO Ⅰ조교대조Ⅰ조RVEDP강저,HBOⅢ조교대조Ⅲ조mPAP、RVEDP、-dp/dtmax강저,차이균유통계학의의(P＜0.05);여HBO Ⅰ조비교,HBO Ⅱ조mPAP、±dp/dtmax강저(P＜0.05、P＜0.01),HBO Ⅲ조RVSP(P ＜0.05)、RVEDP、±dp/dtmax、mPAP강저,차이유통계학의의(P＜0.01);여HBO Ⅱ조비교,HBO Ⅲ조RVEDP강저,차이유통계학의의(P＜0.01).(2) HBO Ⅰ조LVEDP교대조Ⅰ조강저,HBO Ⅲ조LVEDP교대조조Ⅲ조강저,차이균유통계학의의(P ＜0.05、P＜0.01);여HBOⅠ조비교,HBO Ⅱ조LVEDP승고(P＜0.05),HBO Ⅲ조LVSP、LVEDP승고(P ＜0.05、0.01),차이균유통계학의의(P＜0.05、P＜0.01).(3) HBO Ⅰ조여대조Ⅰ조비교,SOD증고,IL-6、IL-10、TNF-α강저,차이유통계학의의(P＜0.05).HBO Ⅱ조여대조Ⅱ조비교,SOD증고,IL-6、IL-10、TNF-α강저,차이유통계학의의(P＜0.05혹P＜0.01).HBO Ⅲ조여대조Ⅲ조비교,SOD증고,MDA、IL-6、IL-10、TNF-α강저,차이유통계학의의(P＜0.05).여HBO Ⅰ조비교,HBO Ⅱ조IL-6、TNF-α승고,차이유통계학의의(균P＜0.05);HBO Ⅲ조SOD강저,IL-6、TNF-α승고,차이유통계학의의(균P＜0.05),IL-10、MDA승고,차이유통계학의의(P＜0.01);여HBO Ⅱ조비교,HBO Ⅲ조SOD강저,IL-6、IL-10、TNF-α、MDA승고,차이균유통계학의의(균P＜0.05).결론 HBO예처리가개선결양궤체혈류동역학,사궤체항양화능력증강,제고심실공능화저양내수능력.
Objective To observe the effects of hyperbaric oxygen (HBO) pretreatment on hemodynamics and such cytokines as IL-6,IL-10 and TNF-α in rats with acute hypoxia (at an altitude of 7 000 m).Methods Fifty SD rats were randomly divided into 6 groups:the HBO groups [the HBO group Ⅰ (n =10),Ⅱ (n =10) and Ⅲ(n =10)] and the control groups [the control group Ⅰ (n =6),Ⅱ (n =7) and Ⅲ (n =7)].The animals in the HBO groups were pretreated with HBO for 7 days,while the animals in the control groups received no treatment.The HBO pretreated animals and the animals in all the control groups were exposed to hypobaric pressure at an altitude of 7 000 m for a duration of 12 h,24 h and 72 h respectively.At different time points,the rats in all the groups had abdominal anesthesia with 10％ urethane in the hypobaric chamber,and then a cardiac catheter was inserted via the right external jugular vein into the right ventricle and pulmonary artery.Multi-channel polygraphy was used to monitor the 5-min pulmonary artery pressure curve,the pulmonary artery pressure (PAP),the right ventricular systolic pressure (RVSP) / left ventricular systolic pressure (LVSP),the right diastolic blood pressure (RVEDP) / the left diastolic blood pressure (LVEDP),the right ventricular systolic ventricular isovolumic maximum rate of pressure rise and right ventricular diastolic ventricular isovolumic maximum rate of pressure drop (± dp / dtmax) and other hemodynamic parameters.Blood samples were collected to detect serum levels of interleukin-6 (IL-6),interleukin-10 (IL-10) and tumor necrosis factor-α (TNF-α),SOD and MDA.Results (1) The RVEDP in the animals of the HBO group Ⅰ was decreased with significant difference,when it was compared with thab of the control group Ⅰ (P ＜ 0.05).The mPAP,RVEDP,-dp/dtmax for the animals in the HBO group Ⅲ were decreased,also with significant difference,when they were compared with those of the control group Ⅲ (P ＜ 0.05).The mPAP and ± dp/dtmax for the animals in the HBO group Ⅱ were decreased with significant difference,when they were compared with those of the HBO group Ⅰ (P ＜ 0.05 or P ＜ 0.01).The RVSP,RVEDP ± dp/dt mPAP in the animals of the HBO group Ⅲ was lower than that of the HBO group Ⅱ with very significant difference (P ＜ 0.01).(2) The LVEDP in the animals of the HBO group Ⅰ was lower than that in the animals of the control group Ⅰ,with significant difference(P ＜0.05).The LVEDP in the animals of the HBO group Ⅲ was lower than that in the animals of the control group Ⅲ,with very significant difference(P ＜0.01).The LVEDP in the animals of the HBO group Ⅱ was increased with significant difference(P ＜0.05),the LVSP and RVEDP in the animals of the HBO group Ⅲ was also increased with significant difference (P ＜ 0.05 or P ＜ 0.01),when they were compared with those of the HBO groud Ⅰ.(3) The SOD level in the animals of the HBO group Ⅰ was higher than that in the animals of the control group Ⅰ,with significant difference,while the levels of IL-6,IL-10 and TNF-α were lower than those of the control group Ⅱ,also with significant difference(P ＜ 0.05 or P ＜ 0.01).The SOD level in the animals of the HBO group Ⅱ was higher than that in the animals of the control group Ⅱ,while the levels of IL-6,IL-10 and TNF-α were lower than those of the control group Ⅱ,also with significant difference(P ＜ 0.05 or P ＜ 0.01).The SOD level in the animals of the HBO group Ⅲ was higher than that in the animals of the control group Ⅲ,while the levels of MDA,IL-6,IL-10 and TNF-α were lower than those of the control group Ⅲ,also with significant difference(P ＜0.05).The IL-6 and TNF-α levels in the animals of the HBO group Ⅰ was lower than that in the animals of the HBO group Ⅱ,with significant difference (P ＜0.05).The SOD level in the animals of the HBO group Ⅰ was higher than that in the animals of the HBO group Ⅲ,while the levels of IL-6 and TNF-αα were lower,also with significant difference(P ＜0.05),and the levels of IL-10 and MDA were lower,with very significant difference(P ＜ 0.01).The SOD level in the animals of the HBO group Ⅱ was higher than that in the animals of the HBO group Ⅲ,while the levels of IL-6,IL-10,TNF-α and MDA were lower,with significant difference (P ＜ 0.05).Conclusions HBO pretreatment could improve poor hemodynamics of the animals induced by hypoxia,enhance antioxidant capacity of the body and increase ventricular function and hypoxia tolerance.
