中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2014年
5期
306-311
,共6页
脑出血%新生血管%高压氧%行为学评分%缺氧诱导因子%血管内皮生长因子%增殖细胞核抗原%Ⅷ因子相关抗原
腦齣血%新生血管%高壓氧%行為學評分%缺氧誘導因子%血管內皮生長因子%增殖細胞覈抗原%Ⅷ因子相關抗原
뇌출혈%신생혈관%고압양%행위학평분%결양유도인자%혈관내피생장인자%증식세포핵항원%Ⅷ인자상관항원
Intracerebral hemorrhage%Angiogenesis%Hyperbaric oxygen%Behavior score%HIF-1a%VEGF%Proliferating cell nuclear antigen%yon Willebrand factor
目的 研究高压氧对脑出血大鼠脑内血管新生因子的影响及作用机制.方法 将60只大鼠按数字表法随机分为假手术组、脑出血组和高压氧组,每组20只大鼠.观察各组大鼠行为学改变情况;检测各组大鼠脑内增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)、Ⅷ因子相关抗原(von Willebrand factor,vWF)、缺氧诱导因子-1(hypoxia inducible factor-1,HIF-1)、血管内皮生长因子(vascular endothelial growth factor,VEGF)的蛋白表达量.结果 实验大鼠行为学评分,高压氧组与脑出血组比较14、21、28 d差异均有统计学意义(P＜0.01);血肿周围脑组织的HIF1-α蛋白表达均高于脑出血组,2组14、21、28 d比较差异有统计学意义(P＜0.01);血肿周围脑组织的PCNA蛋白表达高压氧组均高于脑出血组,7、14、21、28 d差异均有统计学意义(P＜0.05);VEGF蛋白表达高压氧组高于脑出血组,2组14、21、28 d比较差异均有统计学意义(P＜0.01);高压氧组vWF蛋白表达均高于脑出血组,2组7、14、21、28 d比较差异均有统计学意义(P＜0.01).结论 高压氧能通过促进血管新生相关因子HIF1-α、VEGF、PCNA及vWF蛋白的表达,促进脑出血损伤组织血管的新生,增加血肿吸收,促进行为能力的恢复和机体的康复.
目的 研究高壓氧對腦齣血大鼠腦內血管新生因子的影響及作用機製.方法 將60隻大鼠按數字錶法隨機分為假手術組、腦齣血組和高壓氧組,每組20隻大鼠.觀察各組大鼠行為學改變情況;檢測各組大鼠腦內增殖細胞覈抗原(proliferating cell nuclear antigen,PCNA)、Ⅷ因子相關抗原(von Willebrand factor,vWF)、缺氧誘導因子-1(hypoxia inducible factor-1,HIF-1)、血管內皮生長因子(vascular endothelial growth factor,VEGF)的蛋白錶達量.結果 實驗大鼠行為學評分,高壓氧組與腦齣血組比較14、21、28 d差異均有統計學意義(P＜0.01);血腫週圍腦組織的HIF1-α蛋白錶達均高于腦齣血組,2組14、21、28 d比較差異有統計學意義(P＜0.01);血腫週圍腦組織的PCNA蛋白錶達高壓氧組均高于腦齣血組,7、14、21、28 d差異均有統計學意義(P＜0.05);VEGF蛋白錶達高壓氧組高于腦齣血組,2組14、21、28 d比較差異均有統計學意義(P＜0.01);高壓氧組vWF蛋白錶達均高于腦齣血組,2組7、14、21、28 d比較差異均有統計學意義(P＜0.01).結論 高壓氧能通過促進血管新生相關因子HIF1-α、VEGF、PCNA及vWF蛋白的錶達,促進腦齣血損傷組織血管的新生,增加血腫吸收,促進行為能力的恢複和機體的康複.
목적 연구고압양대뇌출혈대서뇌내혈관신생인자적영향급작용궤제.방법 장60지대서안수자표법수궤분위가수술조、뇌출혈조화고압양조,매조20지대서.관찰각조대서행위학개변정황;검측각조대서뇌내증식세포핵항원(proliferating cell nuclear antigen,PCNA)、Ⅷ인자상관항원(von Willebrand factor,vWF)、결양유도인자-1(hypoxia inducible factor-1,HIF-1)、혈관내피생장인자(vascular endothelial growth factor,VEGF)적단백표체량.결과 실험대서행위학평분,고압양조여뇌출혈조비교14、21、28 d차이균유통계학의의(P＜0.01);혈종주위뇌조직적HIF1-α단백표체균고우뇌출혈조,2조14、21、28 d비교차이유통계학의의(P＜0.01);혈종주위뇌조직적PCNA단백표체고압양조균고우뇌출혈조,7、14、21、28 d차이균유통계학의의(P＜0.05);VEGF단백표체고압양조고우뇌출혈조,2조14、21、28 d비교차이균유통계학의의(P＜0.01);고압양조vWF단백표체균고우뇌출혈조,2조7、14、21、28 d비교차이균유통계학의의(P＜0.01).결론 고압양능통과촉진혈관신생상관인자HIF1-α、VEGF、PCNA급vWF단백적표체,촉진뇌출혈손상조직혈관적신생,증가혈종흡수,촉진행위능력적회복화궤체적강복.
Objective To investigate the effects of hyperbaric oxygen (HBO) on intracephalic angiogenesis in rats with intracerebral hemorrhage (ICH) and the mechanism involved.Methods The healthy SD rats were randomly divided into the sham surgery group (group A),the intracerebral hemorrhage group (group B) and the HBO group (group C).Observations were made on changes in rat behavior of each group.Expression levels of proliferating cell nuclear antigen (PCNA),yon Willebrand 's factor (vWF),hypoxia inducible factor-1 (HIF-1 α) and vascular endothelial growth factor (VEGF) were detected in the brain of rats of all the groups.Results Significant differences could be noted in behavior scores of the HBO group at d 14,d 21 and d 28,as compared with those of group B,with obvious statistical significance (P ＜ 0.01).The expression of HIF1-α in the brain tissue around the hematoma was higher than that of group B,and statistical significance could be seen in the expression levels at d 14,d 21 and d 28,when comparisons were made between the 2 groups (P ＜ 0.01).The expression of PCNA in the brain tissue around the hematoma for the animals of the HBO group was higher than that of group B,and statistical significance could be seen in the expression levels at d 14,d 21 and d 28,when comparisons were made between the 2 groups(P ＜ 0.05).The expression of VEGF for the HBO group was higher than that of group B,and statistical significance could be seen in the expression levels at d 14,d 21 and d 28,when comparisons were made between the 2 groups(P ＜0.01).The expression of vWF for the HBO group was also higher than that of group B,and statistical significance could also be seen in the expression levels at d 14,d 21 and d 28 (P ＜ 0.01).Conclusions HBO could accelerate angiogenesis of the damaged tissue induced by cerebral hemorrhage,promote absorption of hematoma,enable recovery of behavioral capacity and enhance rapid rehabilitation of the body,through expressions of such related factors as HIF-1 a,VEGF,PCNA and vWF proteins.
